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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-04-2021 | Uveitis | Inflammatory Disorders

Linoleic acid inhibits in vitro function of human and murine dendritic cells, CD4⁺T cells and retinal pigment epithelial cells

Authors: Xinyue Huang, Shenglan Yi, Jianping Hu, Ziyu Du, Qingfeng Wang, Zi Ye, Guannan Su, Aize Kijlstra, Peizeng Yang

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 4/2021

Abstract

Purpose

Increased linoleic acid (LA) was observed in acute anterior uveitis (AAU) patient feces in our previous study. To investigate the immunoregulatory effect of LA, we studied the effect of LA on human and murine dendritic cells (DCs), CD4⁺T cells, and retinal pigment epithelial (RPE) cells in vitro.

Methods

The level of LA in feces from AAU patients and healthy individuals was measured by gas chromatography coupled with a mass spectrometer (GC-MS). The immunoregulatory effect of LA on human and murine DCs, CD4⁺ T cells, and RPE cells was evaluated by enzyme linked immunosorbent assay (ELISA) and flow cytometry (FCM). The effect of LA on DCs was evaluated by Tandem mass tag (TMT)-based proteomics analysis.

Results

Increased LA was observed in feces from AAU patients (1018.35 ± 900.01 mg/kg) as compared with healthy individuals (472.55 ± 365.49 mg/kg, p = 0.0136). LA attenuated the antigen-presenting function of human and murine DCs by decreasing the expression of CD40, the secretion of IL-6 and IL-12p70, and the ability to shift naïve T cells towards T helper type 1 (Th1) and Th17 cells. LA also inhibited the secretion of MCP-1 and IL-8 from RPE cells. Proteomics analysis showed differential expression of 28 proteins, including squalene epoxidase (SQLE), farnesyl-diphosphate farnesyltransferase 1 (FDFT1), and cytochrome P450 family 51 subfamily A member 1 (CYP51A1), in LA-treated DCs compared with controls. LA also accelerated the apoptosis of DCs from healthy individuals.

Conclusion

LA inhibited the function of human and murine DCs, CD4⁺T cells, and RPE cells, regulated the expression of proteins, and promoted the apoptosis of human DCs. These results collectively suggest that LA might decrease the function of immune cells in vitro, and further studies are needed to investigate its role in the pathogenesis of AAU.

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Title: Linoleic acid inhibits in vitro function of human and murine dendritic cells, CD4+T cells and retinal pigment epithelial cells
Authors: Xinyue Huang
Shenglan Yi
Jianping Hu
Ziyu Du
Qingfeng Wang
Zi Ye
Guannan Su
Aize Kijlstra
Peizeng Yang
Publication date: 01-04-2021
Publisher: Springer Berlin Heidelberg
Keyword: Uveitis
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 4/2021
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-020-04972-6

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Linoleic acid inhibits in vitro function of human and murine dendritic cells, CD4⁺T cells and retinal pigment epithelial cells

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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