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Graefe's Archive for Clinical and Experimental Ophthalmology
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology 3/2019

01-03-2019 | Vitrectomy | Retinal Disorders

A new dye based on anthocyanins from the acai fruit (Euterpe oleracea) for chromovitrectomy in humans: clinical trial results

Authors: Rafael R. Caiado, Cristiane Peris, Eduardo B. Rodrigues, Michel Eid Farah, André Maia, Octaviano Magalhães Jr, Eduardo Novais, Acácio Souza Lima-Filho, Mauricio Maia

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 3/2019

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Abstract

Purpose

To test the applicability of the acai dye at a 25% concentration for identifying the posterior hyaloids and internal limiting membranes (ILMs) during pars plana vitrectomy (PPV) in human eyes with macular holes (MHs).

Methods

This study included 25 patients with chronic idiopathic MHs. The exclusion criteria included glaucoma, previous significant ocular conditions, and previous ocular surgeries except uncomplicated cataract. Ten surgeons performed 23-gauge four-port PPV, phacoemulsification, posterior hyaloid detachment, ILM peeling guided by dye staining, and perfluoropropane injection. The patients remained prone for 5 days postoperatively. The patients were evaluated postoperatively after 1, 30, and 180 days. The surgeons completed a questionnaire regarding the dye’s staining abilities.

Results

The posterior hyaloids and ILMs stained purple in all eyes. The final best-corrected visual acuity improved significantly (p < 0.001) from preoperatively (1.37 ± 0.29) to 180 days postoperatively (1.05 ± 0.43). The MHs closed in 76% of eyes.

Conclusion

The acai dye at a 25% concentration identified posterior hyaloids and ILMs during PPVs in humans. Dye toxicity was unlikely.
Appendix
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Metadata
Title
A new dye based on anthocyanins from the acai fruit (Euterpe oleracea) for chromovitrectomy in humans: clinical trial results
Authors
Rafael R. Caiado
Cristiane Peris
Eduardo B. Rodrigues
Michel Eid Farah
André Maia
Octaviano Magalhães Jr
Eduardo Novais
Acácio Souza Lima-Filho
Mauricio Maia
Publication date
01-03-2019
Publisher
Springer Berlin Heidelberg
Published in
Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 3/2019
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-018-04204-y

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