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Published in: Graefe's Archive for Clinical and Experimental Ophthalmology 2/2013

01-02-2013 | Retinal Disorders

Development and preclinical evaluation of a new viewing filter system to control reflection and enhance dye staining during vitrectomy

Authors: Hiroshi Enaida, Yoshiyuki Hachisuka, Yukiyasu Yoshinaga, Yasuhiro Ikeda, Toshio Hisatomi, Shigeo Yoshida, Yusuke Oshima, Kazuaki Kadonosono, Tatsuro Ishibashi

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 2/2013

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Abstract

Purpose

We developed a new artificial image enhancement system and evaluated its usefulness in controlling intraoperative reflection and enhancing of Brilliant Blue G (BBG) staining.

Methods

The system was composed of three kinds of filters (a polarizing filter, a blue-enhancing filter, and a sharp-cut filter Y) and attached to the inferior surface of the operating microscope. Twenty-seven post-mortem extracted porcine eyes were used for a series of examinations. We performed surgery using the 23G-vitrectomy system with a halogen light and xenon lights and compared the reduction of intraoperative reflection under air condition and visibility and BBG contrast with and without this system. The evaluation of images was calculated in CIE 1976 (L*, a*, b*) color space (CIELAB) carried out by ImageJ software. The transmission of each filter and absorbance of BBG was measured by a spectrophotometer. We measured spectral irradiance at each wavelength about each filter from each light source with a spectroradiometer.

Results

Under both light sources, intraoperative reflection was controlled using a polarizing (PL) filter or combination of filters under air condition. Evaluation of the value of L* within the cutter surface was changed by 37.8 % under the halogen light, and 61.6 % (averaged) under the xenon light with inserted filters versus no filter. The BBG intensity difference was obtained with sharp-cut Y filter under both light source and PL with blue enhancing filter under the halogen light using each L*, a*, b* parameter with statistically significant (p < 0.01, 0.05). However, there was a relative decrease in the observation illuminance when the filter inserted according to the attenuation total spectral irradiance.

Conclusions

This system can reduce intraoperative reflections under the air condition and obtain an excellent BBG staining intensity induced by various light sources.
Literature
1.
go back to reference Kadonosono K, Yabuki K, Nishide T, Nomura E, Uchio E, Yamakawa T (2004) Multicoated contact lens for bimanual vitreous surgery without endoillumination. Arch Ophthalmol 122:367–368PubMedCrossRef Kadonosono K, Yabuki K, Nishide T, Nomura E, Uchio E, Yamakawa T (2004) Multicoated contact lens for bimanual vitreous surgery without endoillumination. Arch Ophthalmol 122:367–368PubMedCrossRef
2.
go back to reference Kadonosono K, Kamezawa H, Uchio E, Tamaki Y, Araie M (2006) Bimanual vitreous surgery with slit-beam illumination and a multicoated contact lens. Retina 26:708–709PubMedCrossRef Kadonosono K, Kamezawa H, Uchio E, Tamaki Y, Araie M (2006) Bimanual vitreous surgery with slit-beam illumination and a multicoated contact lens. Retina 26:708–709PubMedCrossRef
3.
4.
go back to reference Rodrigues EB, Maia M, Meyer CH, Penha FM, Dib E, Farah ME (2007) Vital dyes for chromovitrectomy. Curr Opin Ophthalmol 18:179–187PubMedCrossRef Rodrigues EB, Maia M, Meyer CH, Penha FM, Dib E, Farah ME (2007) Vital dyes for chromovitrectomy. Curr Opin Ophthalmol 18:179–187PubMedCrossRef
5.
go back to reference Enaida H, Hisatomi T, Goto Y, Hata Y, Ueno A, Miura M, Kubota T, Ishibashi T (2006) Preclinical investigation of internal limiting membrane staining and peeling using intravitreal Brilliant Blue G. Retina 26:623–630PubMedCrossRef Enaida H, Hisatomi T, Goto Y, Hata Y, Ueno A, Miura M, Kubota T, Ishibashi T (2006) Preclinical investigation of internal limiting membrane staining and peeling using intravitreal Brilliant Blue G. Retina 26:623–630PubMedCrossRef
6.
go back to reference Enaida H, Hisatomi T, Hata Y, Ueno A, Goto Y, Yamada T, Kubota T, Ishibashi T (2006) Brilliant Blue G selectively stains the internal limiting membrane/ brilliant blue G-assisted membrane peeling. Retina 26:631–636PubMedCrossRef Enaida H, Hisatomi T, Hata Y, Ueno A, Goto Y, Yamada T, Kubota T, Ishibashi T (2006) Brilliant Blue G selectively stains the internal limiting membrane/ brilliant blue G-assisted membrane peeling. Retina 26:631–636PubMedCrossRef
7.
go back to reference Shimada H, Nakashizuka H, Hattori T, Mori R, Mizutani Y, Yuzawa M (2009) Double staining with Brilliant Blue G and double peeling for epiretinal membranes. Ophthalmology 116:1370–1376PubMedCrossRef Shimada H, Nakashizuka H, Hattori T, Mori R, Mizutani Y, Yuzawa M (2009) Double staining with Brilliant Blue G and double peeling for epiretinal membranes. Ophthalmology 116:1370–1376PubMedCrossRef
8.
go back to reference Schumann RG, Gandorfer A, Eibl KH, Henrich PB, Kampik A, Haritoglou C (2010) Sequential epiretinal membrane removal with internal limiting membrane peeling in Brilliant Blue G-assisted macular surgery. Br J Ophthalmol 10:1369–1372CrossRef Schumann RG, Gandorfer A, Eibl KH, Henrich PB, Kampik A, Haritoglou C (2010) Sequential epiretinal membrane removal with internal limiting membrane peeling in Brilliant Blue G-assisted macular surgery. Br J Ophthalmol 10:1369–1372CrossRef
9.
go back to reference Kenawy N, Wong D, Stappler T, Romano NR, Das RA, Hebbar G, Dip PG, Prime W, Heimann H, Gibran SK, Sheridan CM, Cheung YH, Hiscott PS (2010) Does the presence of an epiretinal membrane alter the cleavage plane during internal limiting membrane peeling? Ophthalmology 117:320–323PubMedCrossRef Kenawy N, Wong D, Stappler T, Romano NR, Das RA, Hebbar G, Dip PG, Prime W, Heimann H, Gibran SK, Sheridan CM, Cheung YH, Hiscott PS (2010) Does the presence of an epiretinal membrane alter the cleavage plane during internal limiting membrane peeling? Ophthalmology 117:320–323PubMedCrossRef
10.
go back to reference Rich RM, Billmeyer FW Jr (1974) Unit color-difference figures derived from the proposed CIE1976 Lab space. Appl Opt 13:2475–2476PubMedCrossRef Rich RM, Billmeyer FW Jr (1974) Unit color-difference figures derived from the proposed CIE1976 Lab space. Appl Opt 13:2475–2476PubMedCrossRef
11.
go back to reference Yanagi Y, Iriyama A, Jang WD, Kadonosono K (2007) Evaluation of the safety of xenon/bandpass light in vitrectomy using the A2E-laden RPE model. Graefes Arch Clin Ophthalmol 245:677–681CrossRef Yanagi Y, Iriyama A, Jang WD, Kadonosono K (2007) Evaluation of the safety of xenon/bandpass light in vitrectomy using the A2E-laden RPE model. Graefes Arch Clin Ophthalmol 245:677–681CrossRef
12.
go back to reference Kadonosono K, Itoh N, Uchio E, Nakamura S, Ohno S (2000) Staining of internal limiting membrane in macular hole surgery. Arch Ophthalmol 118:1116–1118PubMed Kadonosono K, Itoh N, Uchio E, Nakamura S, Ohno S (2000) Staining of internal limiting membrane in macular hole surgery. Arch Ophthalmol 118:1116–1118PubMed
13.
go back to reference Enaida H, Sakamoto T, Hisatomi T, Goto Y, Ishibashi T (2002) Morphological and functional damage of the retina caused by intravitreous indocyanine green in rat eyes. Graefes Arch Clin Exp Ophthalmol 240:209–213PubMedCrossRef Enaida H, Sakamoto T, Hisatomi T, Goto Y, Ishibashi T (2002) Morphological and functional damage of the retina caused by intravitreous indocyanine green in rat eyes. Graefes Arch Clin Exp Ophthalmol 240:209–213PubMedCrossRef
14.
go back to reference Kawahara S, Hata Y, Miura M, Kita T, Sengoku A, Nakao S, Mochizuki Y, Enaida H, Ueno A, Hafezi-Moghadam A, Ishibashi T (2007) Intracellular events in retinal glial cells exposed to ICG and BBG. Invest Ophthalmol Vis Sci 48:4426–4432PubMedCrossRef Kawahara S, Hata Y, Miura M, Kita T, Sengoku A, Nakao S, Mochizuki Y, Enaida H, Ueno A, Hafezi-Moghadam A, Ishibashi T (2007) Intracellular events in retinal glial cells exposed to ICG and BBG. Invest Ophthalmol Vis Sci 48:4426–4432PubMedCrossRef
15.
go back to reference Morales MC, Freire V, Asumendi A, Araiz J, Herrera I, Castiella G, Corcóstegui I, Corcóstegui G (2010) Comparative effects of six intraocular vital dyes on retinal pigment epithelial cells. Invest Ophthalmol Vis Sci 51:6018–6029PubMedCrossRef Morales MC, Freire V, Asumendi A, Araiz J, Herrera I, Castiella G, Corcóstegui I, Corcóstegui G (2010) Comparative effects of six intraocular vital dyes on retinal pigment epithelial cells. Invest Ophthalmol Vis Sci 51:6018–6029PubMedCrossRef
16.
go back to reference Ueno A, Hisatomi T, Enaida H, Kagimoto T, Mochizuki Y, Goto Y, Kubota T, Hata Y, Ishibashi T (2007) Biocompatibility of Brilliant Blue G in a rat model of subretinal injection. Retina 27:499–504PubMedCrossRef Ueno A, Hisatomi T, Enaida H, Kagimoto T, Mochizuki Y, Goto Y, Kubota T, Hata Y, Ishibashi T (2007) Biocompatibility of Brilliant Blue G in a rat model of subretinal injection. Retina 27:499–504PubMedCrossRef
17.
go back to reference Ejstrup R, la Cour M, Heegaard S, Kiilgaard JF (2011) Toxicity profiles of subretinal indocyanine green, Brilliant Blue G, and triamcinolone acetonide: a comparative study. Graefes Arch Clin Exp Ophthalmol. doi:10.1007/s00417-011-1886-3 Ejstrup R, la Cour M, Heegaard S, Kiilgaard JF (2011) Toxicity profiles of subretinal indocyanine green, Brilliant Blue G, and triamcinolone acetonide: a comparative study. Graefes Arch Clin Exp Ophthalmol. doi:10.​1007/​s00417-011-1886-3
18.
go back to reference Pohl J, Nguyen-Tat M, Pech O, May A, Rabenstein T, Ell C (2007) Computed virtual chromoendoscopy for classification of small colorectal lesions: a prospective comparative study. Am J Gastroenterol 103:562–569PubMedCrossRef Pohl J, Nguyen-Tat M, Pech O, May A, Rabenstein T, Ell C (2007) Computed virtual chromoendoscopy for classification of small colorectal lesions: a prospective comparative study. Am J Gastroenterol 103:562–569PubMedCrossRef
19.
go back to reference Osawa H, Yamamoto H, Yamada N, Yoshizawa M, Sunada K, Kita H, Ajibe H, Satoh K, Sugano K (2009) Diagnosis of endoscopic Barrett’s esophagus by transnasal flexible spectral imaging color enhancement. J Gastroenterol 44:1125–1132PubMedCrossRef Osawa H, Yamamoto H, Yamada N, Yoshizawa M, Sunada K, Kita H, Ajibe H, Satoh K, Sugano K (2009) Diagnosis of endoscopic Barrett’s esophagus by transnasal flexible spectral imaging color enhancement. J Gastroenterol 44:1125–1132PubMedCrossRef
20.
go back to reference Ahn HH, Kim SN, Kye YC (2006) Digital camera images obtained using a light-emitting diode illuminator and their dermatological applications. Skin Res Technol 12:11–17PubMedCrossRef Ahn HH, Kim SN, Kye YC (2006) Digital camera images obtained using a light-emitting diode illuminator and their dermatological applications. Skin Res Technol 12:11–17PubMedCrossRef
21.
go back to reference Bae EJ, Seo SH, Kye YC, Ahn HH (2010) A quantitative assessment of the human skin surface using polarized light digital photography and its dermatologic significance. Skin Res Technol 16:270–274PubMed Bae EJ, Seo SH, Kye YC, Ahn HH (2010) A quantitative assessment of the human skin surface using polarized light digital photography and its dermatologic significance. Skin Res Technol 16:270–274PubMed
22.
go back to reference Sanmartín P, Villa F, Silva B, Cappitelli F, Prieto B (2011) Color measurements as a reliable method for estimating chlorophyll degradation to phaeopigments. Biodegradation 22:763–771PubMedCrossRef Sanmartín P, Villa F, Silva B, Cappitelli F, Prieto B (2011) Color measurements as a reliable method for estimating chlorophyll degradation to phaeopigments. Biodegradation 22:763–771PubMedCrossRef
23.
go back to reference Henrich PB, Priglinger SG, Haritoglou C, Josifova T, Ferreira PR, Strauss RW, Flammer J, Cattin PC (2011) Quantification of contrast recognizability during Brilliant Blue G- and Indocyanine Green-assisted chromovitrectomy. Invest Ophthalmol Vis Sci 52:4345–4349PubMedCrossRef Henrich PB, Priglinger SG, Haritoglou C, Josifova T, Ferreira PR, Strauss RW, Flammer J, Cattin PC (2011) Quantification of contrast recognizability during Brilliant Blue G- and Indocyanine Green-assisted chromovitrectomy. Invest Ophthalmol Vis Sci 52:4345–4349PubMedCrossRef
24.
go back to reference Shibuya K, Hoshino H, Chiyo M, Iyoda A, Yoshida S, Sekine Y, Iizasa T, Saitoh Y, Baba M, Hiroshima K, Ohwada H, Fujisawa T (2003) High magnification bronchovideoscopy combined with narrow band imaging could detect capillary loops of angiogenic squamous dysplasia in heavy smokers at high risk for lung cancer. Thorax 58:989–995PubMedCrossRef Shibuya K, Hoshino H, Chiyo M, Iyoda A, Yoshida S, Sekine Y, Iizasa T, Saitoh Y, Baba M, Hiroshima K, Ohwada H, Fujisawa T (2003) High magnification bronchovideoscopy combined with narrow band imaging could detect capillary loops of angiogenic squamous dysplasia in heavy smokers at high risk for lung cancer. Thorax 58:989–995PubMedCrossRef
25.
go back to reference Hamamoto Y, Endo T, Nosho K, Arimura Y, Sato M, Imai K (2004) Usefulness of narrow-band imaging endoscopy for diagnosis of Barrett’s esophagus. J Gastroenterol 39:14–20PubMedCrossRef Hamamoto Y, Endo T, Nosho K, Arimura Y, Sato M, Imai K (2004) Usefulness of narrow-band imaging endoscopy for diagnosis of Barrett’s esophagus. J Gastroenterol 39:14–20PubMedCrossRef
26.
go back to reference Michels M, Stemberg P Jr (1990) Operating microscope-induced retinal phototoxicity: pathophysiology, clinical manifestations and prevention. Surv Ophthalmol 34:237–252PubMedCrossRef Michels M, Stemberg P Jr (1990) Operating microscope-induced retinal phototoxicity: pathophysiology, clinical manifestations and prevention. Surv Ophthalmol 34:237–252PubMedCrossRef
27.
go back to reference Kweon EY, Ahn M, Lee DW, You IC, Kim MJ, Cho NC (2009) Operating microscope light-induced phototoxic maculopathy after transscleral sutured posterior chamber intraocular lens implantation. Retina 29:1491–1495PubMedCrossRef Kweon EY, Ahn M, Lee DW, You IC, Kim MJ, Cho NC (2009) Operating microscope light-induced phototoxic maculopathy after transscleral sutured posterior chamber intraocular lens implantation. Retina 29:1491–1495PubMedCrossRef
Metadata
Title
Development and preclinical evaluation of a new viewing filter system to control reflection and enhance dye staining during vitrectomy
Authors
Hiroshi Enaida
Yoshiyuki Hachisuka
Yukiyasu Yoshinaga
Yasuhiro Ikeda
Toshio Hisatomi
Shigeo Yoshida
Yusuke Oshima
Kazuaki Kadonosono
Tatsuro Ishibashi
Publication date
01-02-2013
Publisher
Springer-Verlag
Published in
Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 2/2013
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-012-2035-3

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