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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-05-2012 | Medical Ophthalmology

A potential therapeutic strategy for inhibition of ocular neovascularization with a new endogenous protein: rhEDI-8t

Authors: Ling Zhang, Xi Shen, Qing Lu, Qingwei Zhou, Jiaqi Gu, Renbao Gan, Hui Zhang, Xiaodong Sun, Bing Xie

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 5/2012

Abstract

Background

Endogenous angiogenesis inhibitors act as natural negative feedback in the focal area during the neovascularization process, and have less interference on physiological angiogenesis, and thus fewer negative side-effects. These inhibitors are potential candidates to combine with or substitutes for current popular anti-angiogenesis treatments to have synergistic effect. In this study, the effects of recombinant endothelial growth inhibitor protein (rhEDI-8t), a novel endogenous protein originated from collagen VIII, was investigated on ocular neovascularization (NV). Endostatin, a well-identified endogenous angiogenesis inhibitor, was compared in parallel and served as a positive control.

Methods

The inhibitory effect of rhEDI-8t on vascular endothelial cells was evaluated by a human umbilical vascular endothelial cells (HUVEC) proliferation test and a bovine aortic endothelial cells (BAEC) migration experiment. The effect of rhEDI-8t on ocular NV was further investigated in mice with choroidal neovascularization (choroidal NV) induced by laser, ischemic retinopathy and transgenic mice with expression of VEGF in photoreceptors (rho/VEGF) respectively.

Results

RhEDI-8t inhibited the growth of HUVECs and migration of BAECs stimulated by basic fibroblast growth factor (bFGF). Mice intravitreally treated with rhEDI-8t showed a significant reduction of choroidal NV, retinal NV and subretinal NV.

Conclusion

Endogenous angiogenesis inhibitor rhEDI-8t showed a potent anti-angiogenesis effect in both in vitro and in vivo experiments. It contributed to the suppression of ocular NV. The study suggested that rhEDI-8t could be a subsidiary potent therapeutic medicine in addition to anti-VEGF therapy in future clinical anti-angiogenesis treatment.

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Title: A potential therapeutic strategy for inhibition of ocular neovascularization with a new endogenous protein: rhEDI-8t
Authors: Ling Zhang
Xi Shen
Qing Lu
Qingwei Zhou
Jiaqi Gu
Renbao Gan
Hui Zhang
Xiaodong Sun
Bing Xie
Publication date: 01-05-2012
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 5/2012
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-011-1765-y

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

A potential therapeutic strategy for inhibition of ocular neovascularization with a new endogenous protein: rhEDI-8t

Abstract

Background

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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