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Graefe's Archive for Clinical and Experimental Ophthalmology

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Open Access 01-11-2009 | Medical Ophthalmology

Histochemical and immunohistochemical profile of human and rat ocular medial rectus muscles

Authors: Branka Stirn Kranjc, Vika Smerdu, Ida Eržen

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 11/2009

Abstract

Purpose

To compare the organization of human and rat ocular medial recti muscles (MR).

Methods

The cryosections of human and rat MR were processed for myofibrillar ATPase (mATPase), succinate dehydrogenase and glycerol-3-phosphate dehydrogenase. To reveal myosin heavy chain (MyHC) isoforms, specific monoclonal antibodies against MyHC-1/β- slow, α-cardiac (-α), -2a, -2x, -2b, -extraocular (eom), -embryonic (-emb) and -neonatal (-neo) were applied. The MyHC gene expression was studied by in situ hybridization in human muscle.

Results

The muscle fibers were arranged in two distinct layers in both species. In the orbital layer most fibers were highly oxidative and expressed fast MyHC isoforms, whereas slow and oxidative fibers expressed MyHC-1 and -α, some of them also MyHC-2a, -2x, -eom, very rarely -emb, and –neo. In the global layer, slow fibers with very low oxidative and glycolytic activity and three types of fast fibers, glycolytic, oxidative and oxidative-glycolytic, could be distinguished. The slow medium-sized fibers with mATPase activity stable at pH 4.4 expressed mostly MyHC-1 and -α in rat, while in humans they co-expressed MyHC-1 with -2b, -2x, -eom, and -neo. In both species, the fast fibers showed variable mATPase activity after preincubation at pH 9.4, and co-expressed various combinations of MyHC-2b, -2x, -2a and -eom but not -emb and -neo. MyHC-2b expressing fibers were larger and glycolytic, while MyHC-2a expressing fibers were smaller and highly oxidative in both species. To our knowledge, the present study is the first that demonstrated the expression of MyHC-2b in any of human skeletal muscles. Though the expression of MyHC genes did not correlate with the immunohistochemical profile of fibers in human MR, the expression of MyHC-2b gene was undoubtedly confirmed.

Conclusions

Rat MR represent a good model that can be applied to study human MR in experiment or disease, however certain differences are to be expected due to specific oculomotor demands in humans.

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Title: Histochemical and immunohistochemical profile of human and rat ocular medial rectus muscles
Authors: Branka Stirn Kranjc
Vika Smerdu
Ida Eržen
Publication date: 01-11-2009
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 11/2009
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-009-1128-0

At a glance: The STEP trials

Springer Medicine

Histochemical and immunohistochemical profile of human and rat ocular medial rectus muscles

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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