Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Graefe's Archive for Clinical and Experimental Ophthalmology
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology 2/2008

01-02-2008 | Basic Science

Bacterial adhesion to conventional hydrogel and new silicone-hydrogel contact lens materials

Authors: Laurent Kodjikian, Emmanuelle Casoli-Bergeron, Florence Malet, Hélène Janin-Manificat, Jean Freney, Carole Burillon, Joseph Colin, Jean-Paul Steghens

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 2/2008

Login to get access

Abstract

Background

As bacterial adhesion to contact lenses may contribute to the pathogenesis of keratitis, the aim of our study was to investigate in vitro adhesion of clinically relevant bacteria to conventional hydrogel (standard HEMA) and silicone-hydrogel contact lenses using a bioluminescent ATP assay.

Methods

Four types of unworn contact lenses (Etafilcon A, Galyfilcon A, Balafilcon A, Lotrafilcon B) were incubated with Staphylococcus epidermidis (two different strains) and Pseudomonas aeruginosa suspended in phosphate buffered saline (PBS). Lenses were placed with the posterior surface facing up and were incubated in the bacterial suspension for 4 hours at 37°C. Bacterial binding was then measured and studied by bioluminescent ATP assay. Six replicate experiments were performed for each lens and strain.

Results

Adhesion of all species of bacteria to standard HEMA contact lenses (Etafilcon A) was found to be significantly lower than that of three types of silicone-hydrogel contact lenses, whereas Lotrafilcon B material showed the highest level of bacterial binding. Differences between species in the overall level of adhesion to the different types of contact lenses were observed. Adhesion of P. aeruginosa was typically at least 20 times greater than that observed with both S. epidermidis strains.

Conclusions

Conventional hydrogel contact lenses exhibit significantly lower bacterial adhesion in vitro than silicone-hydrogel ones. This could be due to the greater hydrophobicity but also to the higher oxygen transmissibility of silicone–hydrogel lenses.
Literature
1.
Morgan PB, Woods C, Jones D, Efron N, Tan KO, Gonzalez MY, Pesinova A, Grein HJ, Runberg SE, Tranoudis IG, Chandrinos A, Fine P, Montani G, Morani E, Itoi M, Bendoriene J, van der Worp E, Helland M, Phillips G, Belousov V, Barr JT (2007) International contact lens prescribing in 2006. Contact Lens Spectr 22:34–38
2.
Sweeney D (2004) Silicone hydrogels. Continuous-wear contact lenses, 2nd edn. Butterworth-Heinemann, Edinburgh
3.
Kodjikian L, Burillon C, Roques C, Pellon G, Renaud FN, Hartmann D, Freney J (2004) Intraocular lenses, bacterial adhesion and endophthalmitis prevention: a review. Biomed Mater Eng 14:395–409PubMed
4.
Costerton JW, Cheng KJ, Geesey GG, Ladd TI, Nickel JC, Dasgupta M, Marrie TJ (1987) Bacterial biofilms in nature and disease. Annu Rev Microbiol 41:435–464PubMedCrossRef
5.
LeMagrex E, Brisset L, Jacquelin L, Carquin J, Bonnaveiro N (1994) Susceptibility to antibacterials and compared metabolism of suspended bacteria versus embedded bacteria in biofilms. Colloids Surf B Biointerfaces 2:89–95CrossRef
6.
Anwar H, Strap JL, Costerton JW (1992) Establishment of aging biofilms: possible mechanism of bacterial resistance to antimicrobial therapy. Antimicrob Agents Chemother 36:1347–1351PubMed
7.
Kodjikian L, Burillon C, Roques C, Pellon G, Freney J, Renaud F (2003) Bacterial adherence of Staphylococcus epidermidis to intraocular lenses: a bioluminescence and scanning electron microscopy study. Invest Ophthalmol Vis Sci 44:4388–4394PubMedCrossRef
8.
Kodjikian L, Burillon C, Lina G, Roques C, Pellon G, Freney J, Renaud F (2003) Biofilm formation on intraocular lenses by a clinical strain encoding ica locus: a scanning electron microscopy study. Invest Ophthalmol Vis Sci 44:4382–4387PubMedCrossRef
9.
Kodjikian L, Renaud F, Roques C, Garweg J, Pellon G, Freney J, Burillon C (2005) In vitro influence of vancomycin on adhesion of a Staphylococcus epidermidis strain encoding ica locus to intraocular lenses. J Cataract Refract Surg 31:1050–1058PubMedCrossRef
10.
Burillon C, Kodjikian L, Pellon G, Martra A, Freney J, Renaud FN (2002) In vitro study of bacterial adherence to different types of intraocular lenses. Drug Dev Ind Pharm 28:95–99PubMedCrossRef
11.
Ludwicka A, Jansen B, Wadstrom T, Pulverer G (1984) Attachment of staphylococci to various synthetic polymers. Zentralbl Bakteriol Mikrobiol Hyg [A] 256:479–489
12.
Sheehan D (2000) Spectroscopic techniques. In physical biochemistry: principles and applications. Wiley, Chichester, pp 61–120
13.
Champiat D (1992) Biochimiluminescence and biotechnology. Le technoscope de Biofutur 51:8
14.
Campbell A (1988) Chemiluminescence: principles and applications in biology and medicine. VCH, Ellis Horwood Ltd., New York, p. 265
15.
Lundberg F, Gouda I, Larm O, Galin MA, Ljungh A (1998) A new model to assess staphylococcal adhesion to intraocular lenses under in vitro flow conditions. Biomaterials 19:1727–1733PubMedCrossRef
16.
Farber BF, Wolff AG (1993) Salicylic acid prevents the adherence of bacteria and yeast to silastic catheters. J Biomed Mater Res 27:599–602PubMedCrossRef
17.
Wahl J, Katz H, Abrams D (1991) Infectious keratitis in Baltimore. Ann Ophthalmol 23:234–237PubMed
18.
Sharma S, Gopalakrishnan S, Aasuri M, Garg P, Rao G (2003) Trends in contact lens-associated microbial keratitis in Southern India. Ophthalmology 110:138–143PubMedCrossRef
19.
Verhelst D, Koppen C, Looveren JV, Meheus A, Tassignon M (2005) Clinical, epidemiological and cost aspects of contact lens related infectious keratitis in Belgium: results of a seven-year retrospective study. Bull Soc Belge Ophtalmol 297:7–15PubMed
20.
Ahanotu EN, Hyatt MD, Graham MJ, Ahearn DG (2001) Comparative radiolabel and ATP analyses of adhesion of Pseudomonas aeruginosa and Staphylococcus epidermidis to hydrogel lenses. Clao J 27:89–93PubMed
21.
Bruinsma GM, van der Mei HC, Busscher HJ (2001) Bacterial adhesion to surface hydrophilic and hydrophobic contact lenses. Biomaterials 22:3217–3224PubMedCrossRef
22.
George M, Ahearn D, Pierce G, Gabriel M (2003) Interactions of Pseudomonas aeruginosa and Staphylococcus epidermidis in adhesion to a hydrogel. Eye Contact Lens 29:S105–S109 discussion S115–118, S192–S194PubMedCrossRef
23.
Henriques M, Sousa C, Lira M, Elisabete M, Oliveira R, Azeredo J (2005) Adhesion of Pseudomonas aeruginosa and Staphylococcus epidermidis to silicone-hydrogel contact lenses. Optom Vis Sci 82:446–450PubMedCrossRef
24.
25.
Schnider C, Steffen R (2005) New generation of silicon-hydrogel contact lenses for daily wear. Oftalmologia 49:78–81PubMed
26.
27.
Pascual A, Fleer A, Westerdaal NA, Verhoef J (1986) Modulation of adherence of coagulase-negative staphylococci to Teflon catheters in vitro. Eur J Clin Microbiol 5:518–522PubMedCrossRef
28.
Ruther P, Vincent R (1980) The adhesion of microorganisms to surfaces, physico–chemical aspects. In: Berkeley RCW, Melling LJJ, Rutter PR, Vincent B (eds) Microbial adhesion to surfaces. Ellis Horwwod, London, pp 79–91
29.
Magnusson KE (1982) Hydrophobic interaction–a mechanism of bacterial binding. Scand J Infect Dis Suppl 33:32–36PubMed
30.
Weikart CM, Matsuzawa Y, Winterton L, Yasuda HK (2001) Evaluation of plasma polymer-coated contact lenses by electrochemical impedance spectroscopy. J Biomed Mater Res 54:597–607PubMedCrossRef
31.
Lopez-Alemany A, Compan V, Refojo MF (2002) Porous structure of purevision versus Focus Night&Day and conventional hydrogel contact lenses. J Biomed Mater Res 63:319–325PubMedCrossRef
32.
Jones L, Long J (2002) The impact of contact lens care regimens on the in vitro wettability of conventional and silicone-hydrogel contact lens materials. Invest Ophthalmol Vis Sci: ARVO abstract # 3097
33.
Cheng L, Muller SJ, Radke CJ (2004) Wettability of silicone-hydrogel contact lenses in the presence of tear-film components. Curr Eye Res 28:93–108PubMedCrossRef
34.
de Beer D, Stoodley P, Roe F, Lewandowski Z (1994) Effects of biofilm structures on oxygen distribution and mass transport. Biotechnol Bioeng 43:1131–1138CrossRef
35.
Zhang T, Bishop P (1994) Density, porosity, and pore structure of biofilms. Wat Res 28:2267–2277CrossRef
36.
Pitt WG, Ross SA (2003) Ultrasound increases the rate of bacterial cell growth. Biotechnol Prog 19:1038–1044PubMedCrossRef
37.
Madigan MC, Holden BA (1992) Reduced epithelial adhesion after extended contact lens wear correlates with reduced hemidesmosome density in cat cornea. Invest Ophthalmol Vis Sci 33:314–323PubMed
38.
Cavanagh HD, Ladage PM, Li SL, Yamamoto K, Molai M, Ren DH, Petroll WM, Jester JV (2002) Effects of daily and overnight wear of a novel hyper oxygen-transmissible soft contact lens on bacterial binding and corneal epithelium: a 13-month clinical trial. Ophthalmology 109:1957–1969PubMedCrossRef
39.
Morgan PB, Efron N, Hill EA, Raynor MK, Whiting MA, Tullo AB (2005) Incidence of keratitis of varying severity among contact lens wearers. Br J Ophthalmol 89:430–436PubMedCrossRef
40.
Garcia-Saenz MC, Arias-Puente A, Fresnadillo-Martinez MJ, Paredes-Garcia B (2002) Adherence of two strains of Staphylococcus epidermidis to contact lenses. Cornea 21:511–515PubMedCrossRef
41.
Vermeltfoort PB, Rustema-Abbing M, de Vries J, Bruinsma GM, Busscher HJ, van der Linden ML, Hooymans JM, van der Mei HC (2006) Influence of day and night wear on surface properties of silicone hydrogel contact lenses and bacterial adhesion. Cornea 25:516–523PubMedCrossRef
42.
Butrus S, Klotz S, Misra R (1987) The adherence of Pseudomonas aeruginosa to soft contact lenses. Ophthalmology 94:1310–1314PubMed
Metadata
Title
Bacterial adhesion to conventional hydrogel and new silicone-hydrogel contact lens materials
Authors
Laurent Kodjikian
Emmanuelle Casoli-Bergeron
Florence Malet
Hélène Janin-Manificat
Jean Freney
Carole Burillon
Joseph Colin
Jean-Paul Steghens
Publication date
01-02-2008
Publisher
Springer-Verlag
Published in
Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 2/2008
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-007-0703-5

Other articles of this Issue 2/2008

Graefe's Archive for Clinical and Experimental Ophthalmology 2/2008 Go to the issue

Glaucoma

Mitomycin C induces multidrug resistance in glaucoma surgery

Basic Science

Transplantation of cells from eye-like structures differentiated from embryonic stem cells in vitro and in vivo regeneration of retinal ganglion-like cells

Cornea

Antifungal efficacy of voriconazole, itraconazole and amphotericin b in experimental fusarium solani keratitis

Book Review

Ming Wang (ed.): Corneal topography in the wavefront era: a guide for clinical application

Trauma

Incidence and visual outcome of endophthalmitis associated with intraocular foreign bodies

Trauma

Intraocular pressure rise during 25-gauge vitrectomy trocar placement