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Published in: Graefe's Archive for Clinical and Experimental Ophthalmology 11/2006

01-11-2006 | Clinical Investigation

Quantification of retinal nerve fiber layer thickness reduction associated with a relative afferent pupillary defect

Authors: Yoriko Nakanishi, Makoto Nakamura, Yasuko Tatsumi, Azusa Nagai-Kusuhara, Akira Negi

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 11/2006

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Abstract

Background

A relative afferent pupillary defect (RAPD) is known to develop only when more than 25% of retinal ganglion cells are ablated in monkeys’ eyes. However, there was no prior study to estimate biometrically the degree of retinal nerve fiber layer (RNFL) thickness reduction leading to the development of RAPD in live human eyes. The purpose of this study was to examine the correlation between the amount of RNFL thickness reduction and the depth of a clinically detectable RAPD in patients with unilateral optic atrophy.

Methods

Enrolled were 20 patients with optic atrophy of various etiologies. We quantified RAPD by performing the swinging flashlight test with log-scaled neutral density filters placed over the unaffected eye. Average RNFL thickness was measured by OCT3000 with the average RNFL thickness program. Linear regression analysis was used in assessing the relationship between RAPD and the ratio of affected to unaffected average RNFL thickness.

Results

The mean of average RNFL thickness was 95.6±17.3 μm in the unaffected eyes and 50.7±19.3 μm in the affected eyes (P<0.001). Regression analysis between RAPD and the ratio of affected to unaffected average RNFL thickness revealed a correlation coefficient R2=0.48 (P=0.0007). The regression line intersected the y-axis at 0.77.

Conclusions

RAPD was not clinically detected until at least approximately 25% of the retinal nerve fibers were lost when compared with the unaffected eyes. Substantial retinal ganglion cell damage is required for the development of RAPD.
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Metadata
Title
Quantification of retinal nerve fiber layer thickness reduction associated with a relative afferent pupillary defect
Authors
Yoriko Nakanishi
Makoto Nakamura
Yasuko Tatsumi
Azusa Nagai-Kusuhara
Akira Negi
Publication date
01-11-2006
Publisher
Springer-Verlag
Published in
Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 11/2006
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-006-0327-1

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