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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-04-2006 | Laboratory Investigation

Nerve growth factor modulates in vitro the expression and release of TGF-β1 by amniotic membrane

Authors: Marco Coassin, Alessandro Lambiase, Alessandra Micera, Paola Tirassa, Luigi Aloe, Stefano Bonini

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 4/2006

Abstract

Purpose

To determinate the basal production of NGF and TGF-β1 by amniotic membrane (AM) and to verify the presence of NGF receptors (trkA^NGFR and p75^NTR) in AM. Thereafter, to evaluate in an in vitro model if increasing concentrations of NGF are able to stimulate AM to produce and release TGF-β1.

Methods

Immunohistochemistry, ELISA, in situ hybridization and PCR analysis for NGF, TGF-β1, trkA^NGFR and p75^NTR were performed to study their presence in AM. Amniotic membranes were stimulated in vitro with increasing concentrations of NGF. After 24 h, the amount of TGF- β1 in the AM tissue and in the culture medium was investigate.

Results

AM expressed TGF-β1, NGF, trkA^NGFR and p75^NTR mRNAs and proteins and released basal amounts of NGF and TGF-β1 in the medium. Stimulation of AM by addition of NGF induced a significant (P<0.05) increase of TGF-β1 mRNA/protein in AM tissue as well as a release of TGF-β1 protein into the culture medium, in a dose-dependent fashion.

Conclusions

This study demonstrates that amniotic membrane releases basal amounts of NGF and TGF-β1 and expresses NGF receptors . Moreover, the addition of NGF to the culture medium stimulates the amniotic membrane to significantly increase the production and release of TGF-β1.

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Title: Nerve growth factor modulates in vitro the expression and release of TGF-β1 by amniotic membrane
Authors: Marco Coassin
Alessandro Lambiase
Alessandra Micera
Paola Tirassa
Luigi Aloe
Stefano Bonini
Publication date: 01-04-2006
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 4/2006
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-005-0098-0

At a glance: The STEP trials

Springer Medicine

Nerve growth factor modulates in vitro the expression and release of TGF-β1 by amniotic membrane

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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