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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-07-2005 | Clinical Investigation

Homocysteine and nitric oxide levels in plasma of patients with pseudoexfoliation syndrome, pseudoexfoliation glaucoma, and primary open-angle glaucoma

Authors: Özgül Altintaş, Hale Maral, Nurşen Yüksel, V. Levent Karabaş, Meltem Ö. Dillioğlugil, Yusuf Çağlar

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 7/2005

Abstract

Purpose

To evaluate plasma total homocysteine (tHcy) and nitric oxide (NO) marker levels in patients with pseudoexfoliation syndrome (PXS), pseudoexfoliation glaucoma (PXG), primary open-angle glaucoma (POAG), and normal controls.

Methods

This cross-sectional, prospective study involved 19 patients with POAG, 18 with PXS, 22 with PXG, and 20 control subjects. Fasting tHcy levels of all study participants were determined using a fluorescence polarization immunoassay method. Quantitation of total nitrate was based on the Griess reaction, in which a chromophore with a strong absorbance at 545 nm is formed by reaction of nitrite with a mixture of naphthylethylenediamine and sulphanilamide.

Results

The mean plasma homocysteine level was statistically significantly elevated in the PXS (p=0.033) and the PXG (p=0.023) groups but not in the POAG group (p=0.996) when compared with the control group. Multiple logistic regression analyses comparing the various patient groups with the single control group indicated that elevation in plasma homocysteine concentration was a significant risk factor for PXS (odds ratio per 1 μmol/l increase in homocysteine concentration=2.05, 95% CI=1.19–3.52) and PXG (odds ratio per 1 μmol/l increase in homocysteine concentration=1.36, 95% CI=1.00–1.85) but was not a significant risk factor for POAG (odds ratio per 1 μmol/l increase in homocysteine concentration=0.99, 95% CI=0.78–1.26). NO markers levels were found to be slightly higher in PXS and PXG patients than control and POAG patients but the differences were not statistically significant (p=0.151). Multiple logistic regression analyses comparing the various patient groups with the single control group indicated that elevation in NO marker concentration was not a significant risk factor for PXS (odds ratio per 1 μmol/l increase in NO concentration=1.00, 95% CI=0.99–1.01), PXG (odds ratio per 1 μmol/l increase in NO concentration=1.00, 95% CI=0.99–1.00) and POAG (odds ratio per 1 μmol/l increase in NO concentration=0.99, 95% CI=0.99–1.00). No statistically significant correlations were observed between plasma tHcy and NO markers in study groups (p>0.05).

Conclusion

Elevated levels of homocysteine in pseudoexfoliation patients with and without glaucoma may partly explain the increased risk of vascular disease among patients with pseudoexfoliation. No significant difference was found in plasma NO markers among the POAG, PXS, PXG, and the control subjects.

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Title: Homocysteine and nitric oxide levels in plasma of patients with pseudoexfoliation syndrome, pseudoexfoliation glaucoma, and primary open-angle glaucoma
Authors: Özgül Altintaş
Hale Maral
Nurşen Yüksel
V. Levent Karabaş
Meltem Ö. Dillioğlugil
Yusuf Çağlar
Publication date: 01-07-2005
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 7/2005
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-004-1097-2

At a glance: The STEP trials

Springer Medicine

Homocysteine and nitric oxide levels in plasma of patients with pseudoexfoliation syndrome, pseudoexfoliation glaucoma, and primary open-angle glaucoma

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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