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Graefe's Archive for Clinical and Experimental Ophthalmology
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology 5/2004

01-05-2004 | Short Communication

Distribution of p27(KIP1), cyclin D1, and proliferating cell nuclear antigen after retinal detachment

Authors: Kazuhiko Yoshida, Satoru Kase, Keiko Nakayama, Hiroyasu Nagahama, Takayuki Harada, Hiromi Ikeda, Chikako Harada, Junko Imaki, Kazuhiro Ohgami, Kenji Shiratori, Shigeaki Ohno, Keiichi I. Nakayama

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 5/2004

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Abstract

Purpose

To examine the expression of the p27(KIP1), cyclin D1, and proliferating cell nuclear antigen (PCNA) in the retina and retinal pigment epithelium (RPE) after retinal detachment.

Methods

Normal eyes and eyes at 2 or 4 days after retinal detachment with the C57B16 mouse were analyzed by immunocytochemistry using anti-p27(KIP1), anti-cyclin D1, and anti-proliferating cell nuclear antigen (PCNA) antibodies as well as anti-glutamate synthetase (GS) antibody.

Results

The p27(KIP1) positive nuclei were distributed in the inner nuclear layer (INL) and the RPE of the normal mice eye. In the INL, p27(KIP1) was detected in the middle sublayer, where the nuclei of glutamate synthetase positive Müller cells were situated. In contrast, cyclin D1 was not detected either in the retina or in the RPE. At 2 and 4 days after the retinal detachment, RPE cells under the detached retina were negative for p27(KIP1) and positive for cyclin D1 and PCNA. In the INL of the detached retina, p27(KIP1) was detected after 2 days, but was not detected after 4 days. In contrast, PCNA was not detected in the INL after 2 days, but was detected after 4 days. Cyclin D1 was detected in the middle sublayer of the INL at both 2 and 4 days after the retinal detachment.

Conclusion

These results suggested that degradation of p27(KIP1) and expression of cyclin D1 was involved in the proliferation of the Müller cells as well as RPE cells after retinal detachment.
Literature
1.
Albanese C, Johnson J, Watanabe G, Eklund N, Vu D, Arnold A, Pestell RG (1995) Transforming p21ras mutants and c-Ets-2 activate the cyclin D1 promoter through distinguishable regions. J Biol Chem 270:23589–23597CrossRefPubMed
2.
Anderson DH, Stern WH, Fisher SK, Erickson PA, Borgula GA (1981) The onset of pigment epithelial proliferation after retinal detachment. Invest Ophthalmol Vis Sci 21:10–16PubMed
3.
Blagosklonny MV, Wu GS, Omura S, el-Deiry WS (1996) Proteasome-dependent regulation of p21WAF1/CIP1 expression. Biochem Biophys Res Commun 227:564–569CrossRefPubMed
4.
Bravo R, Macdonald-Bravo H (1987) Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites. J Cell Biol 105:1549–1554PubMed
5.
Carrano AC, Eytan E, Hershko A, Pagano M (1999) SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat Cell Biol 1:193–199CrossRefPubMed
6.
Fisher SK, Erickson PA, Lewis GP, Anderson DH (1991) Intraretinal proliferation induced by retinal detachment. Invest Ophthalmol Vis Sci 32:1739–1748PubMed
7.
Harada T, Imaki J, Hagiwara M, Ohki K, Takamura M, Ohashi T, Matsuda H, Yoshida K (1995) Phosphorylation of CREB in rat retinal cells after focal retinal injury. Exp Eye Res 61:769–772PubMed
8.
Harada T, Harada C, Watanabe M, Inoue Y, Sakagawa T, Nakayama N, Sasaki S, Okuyama S, Watase K, Wada K, Tanaka K (1998) Functions of the two glutamate transporters GLAST and GLT-1 in the retina. Proc Natl Acad Sci USA 95:4663–4666PubMed
9.
Harper JW, Elledge SJ (1996) Cdk inhibitors in development and cancer. Curr Opin Genet Dev 6:56–64PubMed
10.
Hasegawa M (1958) Restitution of the eye after removal of the retina and lens in the newt, Triturus pyrrhogaster. Embryologia 4:1–32
11.
Ikegami Y, Mitsuda S, Araki M (2002) Neural cell differentiation from retinal pigment epithelial cells of the newt: an organ culture model for the urodele retinal regeneration. J Neuröbiol 150:209–220
12.
Jerdan JA, Pepose JS, Michels RG, Hayashi H, de Bustros S, Sebag M, Glaser BM (1989) Proliferative vitreoretinopathy membranes. An immunohistochemical study. Ophthalmology 96:801–810PubMed
13.
Kato JY, Matsuoka M, Polyak K, Massague J, Sherr CJ (1994) Cyclic AMP-induced G 1 phase arrest mediated by an inhibitor (p27Kip 1) of cyclin dependent kinase 4 activation. Cell 79:487–496PubMed
14.
Kurki P, Vanderlaan M, Dolbeare F, Gray J, Tan EM (1986) Expression of proliferatiog cell nuclear antigen (PCNA)/cyclin during the cell cycle. Exp Cell Res 166:209–219PubMed
15.
Machemer R, van Horn D, Aaberg TM (1978) Pigment epithelial proliferation in human retinal detachment with massive periretinal proliferation. Am J Ophthalmol 85:181–191PubMed
16.
Machemer R, Aaberg TM, Freeman HM, Irvine AR, Lean JS, Michels RM (1991) An updated classification of retinal detachment with proliferative vitreoretinopathy. Am J Ophthalmol 112:159–165PubMed
17.
Moriuchi A, Ido A, Nagata Y, Nagata K, Uto H, Hasuike S, Hori T, Hirono S, Hayashi K, Tsubouchi H (2003) A CRE and the region occupied by a protein induced by growth factors contribute to up-regulation of cyclin D l expression in hepatocytes. Biochem Biophys Res Commun 300:415–421CrossRefPubMed
18.
Ohki K, Yoshida K, Yamakawa A, Harada T, Matsuda H, Imaki J (1995) Jun-b gene expression in rat retinal cells following focal retinal injury. Curr Eye Res 14:1021–1024PubMed
19.
Pagano M, Tam SW, Theodoras AM, Beer-Romero P, Del Sal G, Chau V, Yew PR, Draetta GF, Rolfe M (1995) Role of the ubiquitin-proteasome pathway in regulating abundance of the cyclin-dependent kinase inhibitor p27. Science 269:682–685PubMed
20.
Polyak K, Lee MH, Erdjument-Bromage H, Koff A, Roberts JM, Tempst P, Massague J (1994) Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell 78:59–66PubMed
21.
Sherr CJ (1994) G1 phase progression: cycling on cue. Cell 79:551–555PubMed
22.
Shirane M, Harumiya Y, Ishida N, Hirai A, Miyamoto C, Hatakeyama S, Nakayama K, Kitagawa M (1999) Down-regulation of p27(Kip l) by two mechanisms, ubiquitin-mediated degradation and proteolytic processing. J Biol Chem 274:13886–13993CrossRefPubMed
23.
Thanos D, Maniatis T (1995) NF-kappa B: a lesson in family values. Cell 80:529–532PubMed
24.
Toyoshima H, Hunter T (1994) p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21. Cell 78:67–74PubMed
25.
Tsvetkov LM, Yeh KH, Lee SJ, Sun H, Zhang H (1999) p27(Kip1) ubiquitination and degradation is regulated by the SCF(Skp2) complex through phosphorylated Thr187 in p27. Curr Biol 19:661–664CrossRef
26.
Yoshida K, Muraki Y, Ohki K, Harada T, Ohashi T, Matsuda H, Imaki J (1995) C-fos gene expression in rat retinal cells after focal retinal injury. Invest Ophthalmol Vis Sci 36:251–254PubMed
27.
Yoshida K, Hu Y, Karin M (2000) IkappaB Kinase alpha is essential for development of the mammalian cornea and conjunctiva. Invest Ophthalmol Vis Sci 41:3665–3669PubMed
28.
Yoshida K, Nakayama K, Nagahama H, Harada T, Harada C, Imaki J, Matsuda A, Yamamoto K, Ito M, Ohno S, Nakayama KI (2002) Involvement of p27 (KIP1) degradation by Skp2 in the regulation of proliferation in response to wounding of corneal epithelium. Invest Ophthalmol Vis Sci 43:364–370PubMed
Metadata
Title
Distribution of p27(KIP1), cyclin D1, and proliferating cell nuclear antigen after retinal detachment
Authors
Kazuhiko Yoshida
Satoru Kase
Keiko Nakayama
Hiroyasu Nagahama
Takayuki Harada
Hiromi Ikeda
Chikako Harada
Junko Imaki
Kazuhiro Ohgami
Kenji Shiratori
Shigeaki Ohno
Keiichi I. Nakayama
Publication date
01-05-2004
Publisher
Springer-Verlag
Published in
Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 5/2004
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-004-0861-7

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