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Graefe's Archive for Clinical and Experimental Ophthalmology
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology 10/2003

01-10-2003 | Laboratory Investigation

Metipranolol attenuates lipid peroxidation in rat brain: a comparative study with other antiglaucoma drugs

Authors: José Melena, Neville N. Osborne

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 10/2003

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Abstract.

Background

Free radical production seems to be involved in the pathogenesis of a number of ocular diseases. Certain β-adrenoceptor antagonists display antioxidant properties, but these have not been ascribed to any of the presently used ophthalmic β-adrenoceptor antagonists. Therefore, we examined the influence of ophthalmic β-adrenoceptor antagonists and other antiglaucoma drugs on stimulated lipid peroxidation in rat brain homogenates.

Methods

Lipid peroxidation in rat brain homogenates was stimulated by iron/ascorbate or sodium nitroprusside. Lipid peroxidation was assessed by the formation of thiobarbituric acid reactive species (TBARS).

Results

Of the antiglaucoma drugs tested (brimonidine, carteolol, dorzolamide, latanoprost, levobetaxolol, levobunolol, metipranolol, pilocarpine, timolol, travoprost and unoprostone), only metipranolol and its active metabolite, desacetylmetipranolol, were found to significantly reduce iron/ascorbate-induced lipid peroxidation in rat brain homogenates with IC50 values of 6.9 and 1.1 μM, respectively. Metipranolol and desacetylmetipranolol also concentration-dependently inhibited sodium nitroprusside-stimulated lipid peroxidation in rat brain homogenates, displaying IC50 values of 25.1 and 2.6 μM, respectively.

Conclusion

These data indicate that metipranolol and desacetylmetipranolol exhibit remarkable antioxidant properties, with an effect not dissimilar from the reference antioxidant trolox.
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Metadata
Title
Metipranolol attenuates lipid peroxidation in rat brain: a comparative study with other antiglaucoma drugs
Authors
José Melena
Neville N. Osborne
Publication date
01-10-2003
Publisher
Springer-Verlag
Published in
Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 10/2003
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-003-0726-5

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