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Graefe's Archive for Clinical and Experimental Ophthalmology
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology 5/2003

01-05-2003 | Laboratory Investigation

Calcium antagonists in N-methyl d-aspartate-induced retinal injury

Authors: Murat Kaya, Murat Tunç, Talha Özdemir, İrfan Altuntaş

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 5/2003

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Abstract

Purpose

To detect the neuroprotective role of nimodipine and dantrolene in N-methyl-d-aspartate (NMDA)-induced retinal injury.

Methods

In this study we used two calcium antagonists which have two different modes of action, nimodipine and dantrolene, to prevent NMDA-induced retinal ischemia in guinea pigs. In 40 animals we injected nimodipine (n=10), dantrolene (n=10), a combination of both (n=10) or sterile 0.9% NaCl solution as a placebo (n=10) before intravitreal injection of NMDA. We enucleated one eye of each animal after 48 hours and performed histopathologic examination. We also measured malondialdehyde (MDA) levels in retinal homogenates as a marker of ischemic injury.

Results

Our results indicate that ganglion cells of the retina were preserved mostly by nimodipine, followed by combined nimodipine–dantrolene, and dantrolene respectively. The ganglion cell count was statistically significantly higher in cases where we used calcium antagonists than in the control group (p<0.05). We also found that MDA was significantly reduced by calcium antagonists compared to the control group (p<0.05).

Conclusion

Our data show that nimodipine and dantrolene both have potential neuroprotective effects; nimodipine preserved retinal ganglion cells to a greater extent than dantrolene from NMDA-induced retinal injury.
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Metadata
Title
Calcium antagonists in N-methyl d-aspartate-induced retinal injury
Authors
Murat Kaya
Murat Tunç
Talha Özdemir
İrfan Altuntaş
Publication date
01-05-2003
Publisher
Springer-Verlag
Published in
Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 5/2003
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-002-0610-8

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