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01-11-2020 | Original Article

The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR

Authors: Jinding Liu, Rongshuai Wang, Jie Shi, Xiaojuan Cheng, Ting Hao, Jiangling Guo, Jiaqi Wang, Zidong Liu, Wenyan Li, Haoliang Fan, Keming Yun, Jiangwei Yan, Gengqian Zhang

Published in: International Journal of Legal Medicine | Issue 6/2020

Abstract

Y-chromosomal short tandem repeat (Y-STR) polymorphisms are useful in forensic identification, population genetics, and human structures. However, the current Y-STR systems are limited in discriminating distant relatives in a family with a low discrimination power. Increasing the capacity of detecting Y chromosomal polymorphisms will drastically narrow down the matching number of genealogy populations or pedigrees. In this study, we developed a system containing 17 Y-STRs that are complementary to the current commercially available Y-STR kits. This system was constructed by multiplex PCR with expected sizes of 126–400 bp labeled by different fluorescence molecules (DYS715, DYS709, DYS716, DYS713, and DYS607 labeled by FAM; DYS718, DYS723, DYS708, and DYS714 labeled by JOE; DYS712, DYS717, DYS721, and DYS605 labeled by TAMRA; and DYS719, DYS726, DYS598, and DYS722 labeled by ROX). The system was extensively tested for sensitivity, male specificity, species specificity, mixture, population genetics, and mutation rates following the Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. The genetic data were obtained from eight populations with a total of 1260 individuals. Our results showed that all the 17 Y-STRs are human- and male-specific and include only one copy of the Y-chromosome. The 17 Y-STR system detects 143 alleles and has a high discrimination power (0.996031746). Mutation rates were different among the 17 Y-STRs, ranging from 0.30 to 3.03%. In conclusion, our study provides a robust, sensitive, and cost-effective genotyping method for human identification, which will be beneficial for narrowing the search scope when applied to genealogy searching with the Y-STR DNA databank.

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Title: The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR
Authors: Jinding Liu
Rongshuai Wang
Jie Shi
Xiaojuan Cheng
Ting Hao
Jiangling Guo
Jiaqi Wang
Zidong Liu
Wenyan Li
Haoliang Fan
Keming Yun
Jiangwei Yan
Gengqian Zhang
Publication date: 01-11-2020
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Legal Medicine / Issue 6/2020
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-020-02291-3

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