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01-03-2020 | Polymerase Chain Reaction | Method Paper

The validation study of a novel assay with 30 slow and moderate mutation Y-STR markers for criminal investigation and database applications

Authors: Yongsong Zhou, Tong Xie, Yuxin Guo, Xinglin Mei, Qiong Lan, Yating Fang, Xiaoye Jin, Bofeng Zhu

Published in: International Journal of Legal Medicine | Issue 2/2020

Abstract

The Y chromosome short tandem repeat (Y-STR) haplotyping method has been widely used in forensic applications. However, the existing Y-STR panels are not the ideal tools for criminal investigation and database applications because of their relatively low discriminatory capacity (DC) or high mutation rates. In the present study, the multiplex PCR assay (AGCU Y30) for simultaneous amplification of 30 slowly and moderately mutated Y-STR loci labeled by 6-dye fluorescence was developed and validated. The AGCU Y30 assay was capable of amplification purified DNA from casework and database samples on FTA™ cards in direct amplification module with a 10 μL reaction volume. Furthermore, the genetic diversities and forensic parameters of AGCU Y30 were performed using 719 unrelated male samples, demonstrating its high level of genetic polymorphisms and DC in Nantong Han population. This validation study demonstrated good sensitivity, mixture samples, inhibitor tolerance, precision, and concordance for the AGCU Y30, which is suitable for forensic investigation and database construction.

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Title: The validation study of a novel assay with 30 slow and moderate mutation Y-STR markers for criminal investigation and database applications
Authors: Yongsong Zhou
Tong Xie
Yuxin Guo
Xinglin Mei
Qiong Lan
Yating Fang
Xiaoye Jin
Bofeng Zhu
Publication date: 01-03-2020
Publisher: Springer Berlin Heidelberg
Keyword: Polymerase Chain Reaction
Published in: International Journal of Legal Medicine / Issue 2/2020
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-019-02037-w

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