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International Journal of Legal Medicine
Published in: International Journal of Legal Medicine 3/2017

01-05-2017 | Original Article

Detection of RAGE expression and its application to diabetic wound age estimation

Authors: Xin-Yi Ji, Yang Chen, Guang-Hua Ye, Miao-Wu Dong, Ke-Zhi Lin, Jun-Ge Han, Xiang-Ping Feng, Xing-Biao Li, Lin-Sheng Yu, Yan-Yan Fan

Published in: International Journal of Legal Medicine | Issue 3/2017

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Abstract

With the prevalence of diabetes, it is becoming important to analyze the diabetic wound age in forensic practice. The present study investigated the time-dependent expression of receptor for advanced glycation end products (RAGE) during diabetic wound healing in mice and its applicability to wound age determination by immunohistochemistry, double immunofluorescence, and Western blotting. After an incision was created in genetically diabetic db/db mice and control mice, mice were killed at posttraumatic intervals ranging from 6 h to 14 days, followed by the sampling of wound margin. Compared with control mice, diabetic mice showed the delayed wound healing. In control and diabetic wound specimens, RAGE immunoreactivity was observed in a small number of polymorphonuclear cells (PMNs), a number of macrophages, and fibroblasts. Morphometrically, the positive ratios of RAGE in macrophages or fibroblasts considerably increased in diabetic wounds during late repair, which exceeded 60% at 7 and 10 days post-injury. There were no control wound specimens to show a ratio of >60% in macrophages or fibroblasts. By Western blotting analysis, the ratios of RAGE to GAPDH were >1.4 in all diabetic wound samples from 7 to 10 days post-injury, which were >1.8 at 10 days after injury. By comparison, no control wound specimens indicated a ratio of >1.4. In conclusion, the expression of RAGE is upregulated and temporally distributed in macrophages and fibroblasts during diabetic wound healing, which might be closely involved in prolonged inflammation and deficient healing. Moreover, RAGE is promising as a useful marker for diabetic wound age determination.
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Metadata
Title
Detection of RAGE expression and its application to diabetic wound age estimation
Authors
Xin-Yi Ji
Yang Chen
Guang-Hua Ye
Miao-Wu Dong
Ke-Zhi Lin
Jun-Ge Han
Xiang-Ping Feng
Xing-Biao Li
Lin-Sheng Yu
Yan-Yan Fan
Publication date
01-05-2017
Publisher
Springer Berlin Heidelberg
Published in
International Journal of Legal Medicine / Issue 3/2017
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI
https://doi.org/10.1007/s00414-016-1529-7

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