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01-09-2016 | Original Article

Early markers for myocardial ischemia and sudden cardiac death

Authors: Sara Sabatasso, Patrice Mangin, Tony Fracasso, Milena Moretti, Mylène Docquier, Valentin Djonov

Published in: International Journal of Legal Medicine | Issue 5/2016

Abstract

The post-mortem diagnosis of acute myocardial ischemia remains a challenge for both clinical and forensic pathologists. We performed an experimental study (ligation of left anterior descending coronary artery in rats) in order to identify early markers of myocardial ischemia, to further apply to forensic and clinical pathology in cases of sudden cardiac death. Using immunohistochemistry, Western blots, and gene expression analyses, we investigated a number of markers, selected among those which are currently used in emergency departments to diagnose myocardial infarction and those which are under investigation in basic research and autopsy pathology studies on cardiovascular diseases. The study was performed on 44 adult male Lewis rats, assigned to three experimental groups: control, sham-operated, and operated. The durations of ischemia ranged between 5 min and 24 h. The investigated markers were troponins I and T, myoglobin, fibronectin, C5b-9, connexin 43 (dephosphorylated), JunB, cytochrome c, and TUNEL staining. The earliest expressions (≤30 min) were observed for connexin 43, JunB, and cytochrome c, followed by fibronectin (≤1 h), myoglobin (≤1 h), troponins I and T (≤1 h), TUNEL (≤1 h), and C5b-9 (≤2 h). By this investigation, we identified a panel of true early markers of myocardial ischemia and delineated their temporal evolution in expression by employing new technologies for gene expression analysis, in addition to traditional and routine methods (such as histology and immunohistochemistry). Moreover, for the first time in the autopsy pathology field, we identified, by immunohistochemistry, two very early markers of myocardial ischemia: dephosphorylated connexin 43 and JunB.

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Title: Early markers for myocardial ischemia and sudden cardiac death
Authors: Sara Sabatasso
Patrice Mangin
Tony Fracasso
Milena Moretti
Mylène Docquier
Valentin Djonov
Publication date: 01-09-2016
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Legal Medicine / Issue 5/2016
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-016-1401-9

ACC 2024 Congress

Springer Medicine

Early markers for myocardial ischemia and sudden cardiac death

Abstract

ACC 2024 Congress

Springer Medicine

Abstract

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