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01-09-2015 | Original Article

Length heteroplasmy of the polyC-polyT-polyC stretch in the dog mtDNA control region

Authors: Sophie Verscheure, Thierry Backeljau, Stijn Desmyter

Published in: International Journal of Legal Medicine | Issue 5/2015

Abstract

Previously, the mitochondrial control region of 214 Belgian dogs was sequenced. Analysis of this data indicated length heteroplasmy of the polyT stretch in the polyC-polyT-polyC stretch from positions 16661 to 16674. Nine polyC-polyT-polyC haplotype combinations were observed, consisting of seven major haplotypes (highest signal intensity) combined with minor haplotypes (lower signal intensity) one T shorter than the major haplotype in all but three dogs. The longer the polyT stretch, the smaller was the difference in signal intensity between the major and minor haplotype peaks. Additional sequencing, cloning, and PCR trap experiments were performed to further study the intra-individual variation of this mitochondrial DNA (mtDNA) region. Cloning experiments demonstrated that the proportion of clones displaying the minor haplotypes also increased with the length of the polyT stretch. Clone amplification showed that in vitro polymerase errors might contribute to the length heteroplasmy of polyT stretches with at least 10 Ts. Although major and minor polyC-polyT-polyC haplotypes did not differ intra-individually within and between tissues in this study, interpretation of polyT stretch variation should be handled with care in forensic casework.

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Title: Length heteroplasmy of the polyC-polyT-polyC stretch in the dog mtDNA control region
Authors: Sophie Verscheure
Thierry Backeljau
Stijn Desmyter
Publication date: 01-09-2015
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Legal Medicine / Issue 5/2015
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-014-1106-x

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Length heteroplasmy of the polyC-polyT-polyC stretch in the dog mtDNA control region

Abstract

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