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European Archives of Oto-Rhino-Laryngology
Published in: European Archives of Oto-Rhino-Laryngology 12/2019

01-12-2019 | Pentoxifylline | Head and Neck

Effects of pentoxifylline and tocopherol on a rat-irradiated jaw model using micro-CT cortical bone analysis

Authors: Truc Thi Hoang Nguyen, Mi Young Eo, Mi Hyun Seo, Hoon Myoung, Soung Min Kim, Jong Ho Lee

Published in: European Archives of Oto-Rhino-Laryngology | Issue 12/2019

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Abstract

Purpose

A combination of pentoxifylline (PTX) and tocopherol (TP) is believed to reduce chronic fibrosis and induce bone healing in osteoradionecrosis (ORN) of the mandible, but evidence of its therapeutic effectiveness for cortical bone is lacking. This study was designed to determine the effect of combined PTX and TP (PTX + TP) on mandibular cortical bone remodeling in a rat model of ORN, using micro-CT and histological analysis.

Methods

Forty-eight 8-week-old male Sprague–Dawley rats were randomly divided into irradiated (n = 40) and non-irradiated (n = 8) groups. Animals in the irradiated group were divided into four sub-groups, including PTX, TP, PTX + TP, and normal saline. Three weeks after irradiation, mandibular posterior tooth extraction was performed, and animals were sacrificed 7 weeks after irradiation. The mandibles were analyzed using micro-CT and histological evaluation.

Results

The alveolar bone height, cortical bone thickness, cortical bone volume, and total cortical bone surface of the PTX + TP group were significantly greater than those of other irradiated groups (p < 0.05). In 3D reconstructed images, the residual volumes of cortical and cancellous bone were inadequate in the irradiated groups.

Conclusion

We found that a combination of PTX and TP improved quality and quantity of cortical bone in irradiated rat mandibles, thus providing supporting evidence of its utility as a treatment and prophylactic agent in ORN. We observed inadequate volumes of cortical and cancellous bone in ORN mandibles, suggesting that cortical bone could play an important role in further ORN studies.
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Metadata
Title
Effects of pentoxifylline and tocopherol on a rat-irradiated jaw model using micro-CT cortical bone analysis
Authors
Truc Thi Hoang Nguyen
Mi Young Eo
Mi Hyun Seo
Hoon Myoung
Soung Min Kim
Jong Ho Lee
Publication date
01-12-2019
Publisher
Springer Berlin Heidelberg
Keyword
Pentoxifylline
Published in
European Archives of Oto-Rhino-Laryngology / Issue 12/2019
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI
https://doi.org/10.1007/s00405-019-05600-8

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