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European Archives of Oto-Rhino-Laryngology
Published in: European Archives of Oto-Rhino-Laryngology 9/2019

01-09-2019 | Shock | Rhinology

Profiling of heat shock proteins 27 and 70 in adenoids of children

Authors: Hyun Jin Min, Joon Soon Park, Chang Eurn Kim, Kyung Soo Kim

Published in: European Archives of Oto-Rhino-Laryngology | Issue 9/2019

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Abstract

Purpose

Heat shock protein (HSP)27 and 70 are molecular chaperones that may have immunomodulatory functions. We determined if and at what levels each are expressed in the adenoids of pediatric subjects. We also examined tissue distributions, associated clinical characteristics, and antibacterial effects.

Methods

Western blot, enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry were applied to adenoidal tissues and lavage fluids obtained from children (N = 40) undergoing adenotonsillectomy.

Results

Via western blot and ELISA, both HSP27 and 70 were regularly detected in adenoidal tissue and in lavage fluid samples. HSP27 was highly expressed in epithelium, whereas HSP70 showed strong subepithelial positivity and bore a significant relation to adenoidal size. Assayed levels of HSP27 and 70 correlated inversely, and their addition to culture media independently increased bacterial numbers (Staphylococcus aureus). Upon the precipitation of each from adenoidal lavage fluids, bacterial counts declined.

Conclusions

HSP27 and 70 are readily expressed in the adenoids of children and may be implicated in immunologic responses.
Literature
1.
Dolen WK, Spofford B, Selner JC (1990) The hidden tonsils of Waldeyer's ring. Ann Allergy 65:244–248PubMed
2.
Sade K, Fishman G, Kivity S, DeRowe A, Langier S (2011) Expression of Th17 and Treg lymphocyte subsets in hypertrophied adenoids of children and its clinical significance. Immunol Invest 40:657–666CrossRefPubMed
3.
Brandtzaeg P (2003) Immunology of tonsils and adenoids: everything the ENT surgeon needs to know. Int J Pediatr Otorhinolaryngol 67(Suppl 1):S69–76CrossRefPubMed
4.
Aktepe F, Sahin O, Dilek H, Yilmaz D, Kahveci O, Derekoy S (2007) Immunohistochemical assesment of heat shock protein 70 in adenoid tissue. Int J Pediatr Otorhinolaryngol 71:857–861CrossRefPubMed
5.
Ogasawara N, Kojima T, Go M, Takano K, Kamekura R, Ohkuni T, Koizumi J, Masaki T, Fuchimoto J, Obata K, Kurose M, Shintani T, Sawada N, Himi T (2011) Epithelial barrier and antigen uptake in lymphoepithelium of human adenoids. Acta Otolaryngol 131:116–123CrossRefPubMed
6.
7.
Yang H, Wang H, Chavan SS, Andersson U (2015) High mobility group box protein 1 (HMGB1): The prototypical endogenous danger molecule. Mol Med 21(Suppl 1):S6–S12CrossRefPubMedPubMedCentral
8.
Nollen EA, Brunsting JF, Roelofsen H, Weber LA, Kampinga HH (1999) In vivo chaperone activity of heat shock protein 70 and thermotolerance. Mol Cell Biol 19:2069–2079CrossRefPubMedPubMedCentral
9.
Gelderblom M, Sobey CG, Kleinschnitz C, Magnus T (2015) Danger signals in stroke. Ageing Res Rev 24:77–82CrossRefPubMed
10.
Min HJ, Kim SJ, Kim TH, Chung HJ, Yoon JH, Kim CH (2015) Level of secreted HMGB1 correlates with severity of inflammation in chronic rhinosinusitis. Laryngoscope 125:E225–230CrossRefPubMed
11.
Choi IJ, Rhee CS, Lee CH, Kim DY (2013) Effect of allergic rhinitis on the expression of human beta-defensin 2 in tonsils. Ann Allergy Asthma Immunol 110:178–183CrossRefPubMed
12.
Min HJ, Yoon JH, Kim CH (2016) HSP70 is associated with the severity of inflammation in chronic rhinosinusitis. Am J Rhinol Allergy 30:E101–E106CrossRef
13.
Kurien M, Lepcha A, Mathew J, Ali A, Jeyaseelan L (2005) X-rays in the evaluation of adenoid hypertrophy: it's role in the endoscopic era. Indian J Otolaryngol Head Neck Surg 57:45–47PubMedPubMedCentral
14.
Liu H-Y, Dicksved J, Lundh T, Lindberg JE (2014) Expression of heat shock proteins 27 and 72 correlates with specific commensal microbes in different regions of porcine gastrointestinal tract. Am J Physiol Gastrointest Liver Physiol 306:G1033–G1041CrossRefPubMed
15.
Malago JJ, Koninkx JFJG, van Dijk JE (2002) The heat shock response and cytoprotection of the intestinal epithelium. Cell Stress Chaperones 7:191–199CrossRefPubMedPubMedCentral
16.
De AK, Kodys KM, Yeh BS, Miller-Graziano C (2000) Exaggerated human monocyte IL-10 Concomitant to minimal TNF-α Induction by heat-shock protein 27 (Hsp27) suggests Hsp27 Is Primarily an antiinflammatory stimulus. J Immunol 165:3951–3958CrossRefPubMed
17.
Borges TJ, Wieten L, van Herwijnen MJ, Broere F, van der Zee R, Bonorino C, van Eden W (2012) The anti-inflammatory mechanisms of Hsp70. Front Immunol 3:95CrossRefPubMedPubMedCentral
18.
19.
Lu X, Kakkar V (2010) The Role of heat shock protein (HSP) in atherosclerosis: Pathophysiology and clinical opportunities. Curr Med Chem 17:957–973CrossRefPubMed
20.
Choi SR, Lee SA, Kim YJ, Ok CY, Lee HJ, Hahm KB (2009) Role of heat shock proteins in gastric inflammation and ulcer healing. J Physiol Pharmacol 60:5–17PubMed
21.
Bozkus F, Sarikaya S, Kocaturk O, Calik M, Abuhandan M, Altintas A, Koca B, Guler OK, Aksoy N, Ulas T (2014) Evaluation of preoperative and postoperative S-100B levels in children with chronic adenotonsillar hypertrophy: preliminary results. Eur Rev Med Pharmacol Sci 18:1549–1553PubMed
22.
Rynnel-Dagoo B, Lindberg K, Samuleson A, Blomberg S, Forsgren J (1997) The immunology of the host-parasite relationship in the nasopharynx. Ann NY Acad Sci 830:32–48CrossRefPubMed
23.
Heilborn JD, Nilsson MF, Kratz G, Weber G, Sorensen O, Borregaard N, Stahle-Backdahl M (2003) The cathelicidin anti-microbial peptide LL-37 is involved in re-epithelialization of human skin wounds and is lacking in chronic ulcer epithelium. J Invest Dermatol 120:379–389CrossRefPubMed
Metadata
Title
Profiling of heat shock proteins 27 and 70 in adenoids of children
Authors
Hyun Jin Min
Joon Soon Park
Chang Eurn Kim
Kyung Soo Kim
Publication date
01-09-2019
Publisher
Springer Berlin Heidelberg
Keywords
Shock
Shock
Published in
European Archives of Oto-Rhino-Laryngology / Issue 9/2019
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI
https://doi.org/10.1007/s00405-019-05528-z

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