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European Archives of Oto-Rhino-Laryngology

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01-07-2019 | Otology

Effect of vascular endothelial growth factor and its receptors in adult otitis media with effusion

Authors: Mengxiao Ye, Xiping Li

Published in: European Archives of Oto-Rhino-Laryngology | Issue 7/2019

Abstract

Purpose

Some studies have demonstrated that vascular endothelial growth factor (VEGF) plays a critical role in the pathogenesis of otitis media with effusion (OME) in animal models. However, the levels of VEGF and its receptors in adult OME have not been clarified. Our study was designed to detect the levels of VEGF and its receptors in adult OME and explore their relationship with effusion types, duration and prognosis of OME.

Methods

61 patients with secretory otitis media were enrolled including 21 males and 40 females, with an average age of 54.7 ± 17.5 years. The middle-ear effusions were collected by tympanocentesis or myringotomy. The protein concentrations were determined by enzyme-linked immunosorbent assay and messenger RNA by real-time quantitative PCR.

Results

VEGF level was higher in AOME group, but not correlated with the recurrence of OME. VEGFR1 and VEGFR2 levels were lower in recurrent group compared with non-recurrent group. VEGFR2 level was higher in serous effusions than mucoid effusions. VEGF messenger RNA was positively correlated both with HIF-1α and MUC5B.

Conclusions

VEGF and its receptors function to induce the production of middle-ear effusions (MEEs) at acute stage of OME rather than chronic or recurrent stage, which is mainly mediated by HIF-1α pathway. The formation of mucoid effusions is associated with MUC5B and VEGFR2, but not with duration and recurrence of OME.

Rosenfeld RM, Shin JJ, Schwartz SR et al (2016) Clinical practice guideline: otitis media with effusion (update). Otolaryngol Head Neck Surg 154(1 Suppl):1–41. https://doi.org/10.1177/0194599815623467 CrossRef

Atkinson H, Wallis S, Coatesworth AP (2015) Otitis media with effusion. Postgrad Med 127(4):381–385. https://doi.org/10.1080/00325481.2015.1028317 CrossRefPubMed

Tang Z, Chen R (2014) Otitis media with effusion in the adult. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 28(13):925–929PubMed

Buzatto GP, Tamashiro E, Proenca-Modena JL, Saturno TH, Prates MC, Gagliardi TB, Carenzi LR, Massuda ET, Hyppolito MA, Valera FC, Arruda E, Anselmo-Lima WT (2017) The pathogens profile in children with otitis media with effusion and adenoid hypertrophy. PLoS One 12(2):e0171049. https://doi.org/10.1371/journal.pone.0171049 CrossRefPubMedPubMedCentral

Roberts J, Hunter L, Gravel J, Rosenfeld R, Berman S, Haggard M, Hall J, Lannon C, Moore D, Vernon-Feagans L, Wallace I (2004) Otitis media, hearing loss, and language learning: controversies and current research. J Dev Behav Pediatr 25(2):110–122CrossRefPubMed

Smith GA, Fearnley GW, Harrison MA, Tomlinson DC, Wheatcroft SB, Ponnambalam S (2015) Vascular endothelial growth factors: multitasking functionality in metabolism, health and disease. J Inherit Metab Dis 38(4):753–763. https://doi.org/10.1007/s10545-015-9838-4 CrossRefPubMed

Shibuya M (2013) Vascular endothelial growth factor and its receptor system: physiological functions in angiogenesis and pathological roles in various diseases. J Biochem 153(1):13–19. https://doi.org/10.1093/jb/mvs136 CrossRefPubMed

Koch S, Tugues S, Li X, Gualandi L, Claesson-Welsh L (2011) Signal transduction by vascular endothelial growth factor receptors. Biochem J 437(2):169–183. https://doi.org/10.1042/BJ20110301 CrossRef

Sun YZ, Cai N, Liu NN (2017) Celecoxib down-regulates the hypoxia-induced expression of HIF-1alpha and VEGF through the PI3K/AKT pathway in retinal pigment epithelial cells. Cell Physiol Biochem 44(4):1640–1650. https://doi.org/10.1159/000485764 CrossRefPubMed

10.

Ferrara NGH, LeCouter J (2003) The biology of VEGF and its receptors. Nature medicine 9(6):669–676CrossRefPubMed

11.

Jung HH (1999) Expression of vascular endothelial growth factor in otitis media. Acta Otolaryngol 1999(119):801–808. https://doi.org/10.1080/00016489950180450 CrossRef

12.

Sekiyama K, Ohori J-I, Matsune S, Kurono Y (2011) The role of vascular endothelial growth factor in pediatric otitis media with effusion. Auris Nasus Larynx 38(3):319–324. https://doi.org/10.1016/j.anl.2010.10.008 CrossRefPubMed

13.

Dor Y, Porat R, Keshet E (2001) Vascular endothelial growth factor and vascular adjustments to perturbations in oxygen homeostasis. Am J Physiol Cell Physiol 280:C1367–C1374CrossRefPubMed

14.

Cheeseman MT, Tyrer HE, Williams D, Hough TA, Pathak P, Romero MR, Hilton H, Bali S, Parker A, Vizor L, Purnell T, Vowell K, Wells S, Bhutta MF, Potter PK, Brown SD (2011) HIF-VEGF pathways are critical for chronic otitis media in Junbo and Jeff mouse mutants. PLoS Genet 7(10):e1002336. https://doi.org/10.1371/journal.pgen.1002336 CrossRefPubMedPubMedCentral

15.

Paradise JL, Rockette HE, Colborn DK, Bernard BS, Smith CG, Kurs-Lasky M, Janosky JE (1997) Otitis media in 2253 Pittsburgh-area infants: prevalence and risk factors during the first two years of life. Pediatrics 99(3):318–333CrossRefPubMed

16.

Poe DS, Pyykko I (2011) Measurements of Eustachian tube dilation by video endoscopy. Otol Neurotol 32(5):794–798. https://doi.org/10.1097/MAO.0b013e31821c6355 CrossRefPubMed

17.

Hsin C-H, Chen T-H, Liang K-L, Tseng H-C, Liu W-S (2013) Postirradiation otitis media with effusion in nasopharyngeal carcinoma patients treated by intensity-modulated radiotherapy. Laryngoscope 123(9):2148–2153. https://doi.org/10.1002/lary.23215 CrossRefPubMed

18.

Madhuri Ramanathan GP-E, Hao I, Leibovich SJ (2007) Synergistic up-regulation of vascular endothelial growth factor (VEGF) expression in macrophages by adenosine A2A receptor agonists and endotoxin involves transcriptional regulation via the hypoxia response element in the VEGF promoter. Mol Biol Cell 18:14–23. https://doi.org/10.1091/mbc.E06 CrossRefPubMedPubMedCentral

19.

Ramanathan M, Luo W, Csoka B, Hasko G, Lukashev D, Sitkovsky MV, Leibovich SJ (2009) Differential regulation of HIF-1alpha isoforms in murine macrophages by TLR4 and adenosine A(2A) receptor agonists. J Leukoc Biol 86(3):681–689. https://doi.org/10.1189/jlb.0109021 CrossRefPubMedPubMedCentral

20.

Sun D, Matsune S, Ohori J, Fukuiwa T, Ushikai M, Kurono Y (2005) TNF-alpha and endotoxin increase hypoxia-induced VEGF production by cultured human nasal fibroblasts in synergistic fashion. Auris Nasus Larynx 32(3):243–249. https://doi.org/10.1016/j.anl.2005.01.004 CrossRefPubMed

21.

Lin J, Caye-Thomasen P, Tono T, Zhang QA, Nakamura Y, Feng L, Huang J, Ye S, Hu X, Kerschner JE (2012) Mucin production and mucous cell metaplasia in otitis media. Int J Otolaryngol 2012:745325. https://doi.org/10.1155/2012/745325 CrossRefPubMedPubMedCentral

22.

Preciado D, Goyal S, Rahimi M, Watson AM, Brown KJ, Hathout Y, Rose MC (2010) MUC5B is the predominant mucin glycoprotein in chronic otitis media fluid. Pediatr Res 68(3):231–236. https://doi.org/10.1203/PDR.0b013e3181eb2ecc (10.1203/00006450-201011001-00451) CrossRefPubMedPubMedCentral

Title: Effect of vascular endothelial growth factor and its receptors in adult otitis media with effusion
Authors: Mengxiao Ye
Xiping Li
Publication date: 01-07-2019
Publisher: Springer Berlin Heidelberg
Published in: European Archives of Oto-Rhino-Laryngology / Issue 7/2019
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-019-05400-0

At a glance: The STEP trials

Springer Medicine

Effect of vascular endothelial growth factor and its receptors in adult otitis media with effusion

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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