Top

European Archives of Oto-Rhino-Laryngology

Published in:

01-02-2017 | Laryngology

β-Defensin-2 is overexpressed in human vocal cord polyps

Authors: Jinjin Gu, Yongwang Huang

Published in: European Archives of Oto-Rhino-Laryngology | Issue 2/2017

Abstract

The objective of the study is to investigate the expression of human β-defensin-1 (hBD-1) and human β-defensin-2 (hBD-2) in vocal cord polyps using tissue microarray. Tissue specimens from vocal cord polyps (N = 51), vocal cord nodules (N = 26), and healthy vocal cords (N = 8) were retrieved from the biobank of the Department of Pathology of Tianjin Tianhe Hospital between 2003 and 2006 and immunostained on tissue microarrays for the quantitative analysis of hBD-1 and hBD-2 expression. hBD-1 expression did not differ significantly between healthy vocal cords, vocal cord nodules, and vocal cord polyps (p = 0.904). In contrast, hBD-2 expression was significantly higher in vocal cord polyps compared to vocal cord nodules and healthy vocal cords (p < 0.001). The expression of hBD-2, but not hBD-1, is elevated in vocal cord polyp epithelium. This suggests that hBD-1 has a more constitutive role in host defense in the vocal cords, whereas hBD-2 expression may be a result of local inflammation or the presence of invading pathogens.

Johns MM (2003) Update on the etiology, diagnosis, and treatment of vocal fold nodules, polyps, and cysts. Curr Opin Otolaryngol Head Neck Surg. 11(6):456–461CrossRefPubMed

Martins RHG, Defaveri J, Domingues MAC, de Albuquerque e Silva R (2010) Vocal polyps: clinical, morphological, and immunohistochemical aspects. J Voice. 25(1):98–106CrossRefPubMed

Ganz T (2002) Epithelia: not just physical barriers. Proc Natl Acad Sci USA. 99(6):3357–3358CrossRefPubMedPubMedCentral

Zhao Le, Lu W (2014) Defensins in innate immunity. Curr Opin Hematol 21(1):37–42CrossRefPubMed

Hans M, Madaan Hans V (2014) Epithelial antimicrobial peptides: guardian of the oral cavity. Int J Pept 2014(1):370297PubMedPubMedCentral

Semple F, Dorin JR (2012) β-Defensins: multifunctional modulators of infection, inflammation and more? J Innate Immun. 4(4):337–348CrossRefPubMed

Pazgier M, Hoover DM, Yang D, Lu W, Lubkowski J (2006) Human beta-defensins. Cell Mol Life Sci 63(11):1294–1313CrossRefPubMed

Chen P-H, Fang S-Y (2004) Expression of human beta-defensin 2 in human nasal mucosa. Eur Arch Otorhinolaryngol 261(5):238–241CrossRefPubMed

Lee SH, Kim JE, Lim HH, Lee HM, Choi JO (2002) Antimicrobial defensin peptides of the human nasal mucosa. Ann Otol Rhinol Laryngol. 111(2):135–141CrossRefPubMed

10.

Abiko Y, Saitoh M, Nishimura M, Yamazaki M, Sawamura D, Kaku T (2007) Role of beta-defensins in oral epithelial health and disease. Med Mol Morphol. 40(4):179–184CrossRefPubMed

11.

McCray PB, Bentley L (1997) Human airway epithelia express a beta-defensin. Am J Respir Cell Mol Biol 16(3):343–349CrossRefPubMed

12.

Marshall A, Hodgson J (1998) DNA chips: an array of possibilities. Nat Biotechnol 16(1):27–31CrossRefPubMed

13.

Kononen J, Bubendorf L, Kallioniemi A, Bärlund M, Schraml P, Leighton S et al (1998) Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med 4(7):844–847CrossRefPubMed

14.

Rimm DL, Camp RL, Charette LA, Olsen DA, Provost E (2001) Amplification of tissue by construction of tissue microarrays. Exp Mol Pathol 70(3):255–264CrossRefPubMed

15.

Nunes RB, Behlau M, Nunes MB, Paulino JG (2013) Clinical diagnosis and histological analysis of vocal nodules and polyps. Braz J Otorhinolaryngol. 79(4):434–440CrossRefPubMed

16.

Cui C, Cheng J, Lin Z, Yi Z, Yang J (2002) Study on tyrosine phosphate gene expression in nasopharyngeal carcinoma. Chin J Otorhinolaryngol. 37(4):315–316

17.

Kong W (2002) Otolaryngology, 2nd edn. People’s Health Publishing House, Beijing

18.

Kambic V, Radsel Z, Zargi M, Acko M (1981) Vocal cord polyps: incidence, histology and pathogenesis. J Laryngol Otol 95(6):609–618CrossRefPubMed

19.

Gray SD, Hammond E, Hanson DF (1995) Benign pathologic responses of the larynx. Ann Otol Rhinol Laryngol. 104(1):13–18CrossRefPubMed

20.

Remacle M, Degols JC, Delos M (1996) Exudative lesions of Reinke’s space. An anatomopathological correlation. Acta Otorhinolaryngol Belg. 50(4):253–264PubMed

21.

Wallis L, Jackson-Menaldi C, Holland W, Giraldo A (2004) Vocal fold nodule vs. vocal fold polyp: answer from surgical pathologist and voice pathologist point of view. J Voice 18(1):125–129CrossRefPubMed

22.

Krisanaprakornkit S, Weinberg A, Perez CN, Dale BA (1998) Expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue. Infect Immun 66(9):4222–4228PubMedPubMedCentral

23.

O’Neil DA, Porter EM, Elewaut D, Anderson GM, Eckmann L, Ganz T et al (1999) Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol. 163(12):6718–6724PubMed

24.

Harder J, Bartels J, Christophers E, Schroder JM (1997) A peptide antibiotic from human skin. Nature 387(6636):861CrossRefPubMed

25.

Ali RS, Falconer A, Ikram M, Bissett CE, Cerio R, Quinn AG (2001) Expression of the peptide antibiotics human beta defensin-1 and human beta defensin-2 in normal human skin. J Invest Dermatol. 117(1):106–111CrossRefPubMed

26.

Liu L, Wang L, Jia HP, Zhao C, Heng HH, Schutte BC et al (1998) Structure and mapping of the human beta-defensin HBD-2 gene and its expression at sites of inflammation. Gene 222(2):237–244CrossRefPubMed

27.

Tsutsumi-Ishii Y, Nagaoka I (2002) NF-kappa B-mediated transcriptional regulation of human beta-defensin-2 gene following lipopolysaccharide stimulation. J Leukoc Biol 71(1):154–162PubMed

28.

Harder J, Meyer-Hoffert U, Teran LM, Schwichtenberg L, Bartels J, Maune S et al (2000) Mucoid Pseudomonas aeruginosa, TNF-alpha, and IL-1beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia. Am J Respir Cell Mol Biol 22(6):714–721CrossRefPubMed

29.

MacRedmond R, Greene C, Taggart CC, McElvaney N, O’Neill S (2005) Respiratory epithelial cells require toll-like receptor 4 for induction of human beta-defensin 2 by lipopolysaccharide. Respir Res 6:116CrossRefPubMedPubMedCentral

30.

Tsutsumi-Ishii Y, Nagaoka I (2003) Modulation of human beta-defensin-2 transcription in pulmonary epithelial cells by lipopolysaccharide-stimulated mononuclear phagocytes via proinflammatory cytokine production. J Immunol. 170(8):4226–4236CrossRefPubMed

31.

Duits LA, Ravensbergen B, Rademaker M, Hiemstra PS, Nibbering PH (2002) Expression of beta-defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells. Annu Rev Immunol 106(4):517–525

32.

O’Neil DA, Cole SP, Martin-Porter E, Housley MP, Liu L, Ganz T et al (2000) Regulation of human beta-defensins by gastric epithelial cells in response to infection with Helicobacter pylori or stimulation with interleukin-1. Infect Immun 68(9):5412–5415CrossRefPubMedPubMedCentral

33.

Tsutsumi-Ishii Y, Nagaoka I (2002) NF-kappa B-mediated transcriptional regulation of human beta-defensin-2 gene following lipopolysaccharide stimulation. J Leukoc Biol 71(1):154–162PubMed

Title: β-Defensin-2 is overexpressed in human vocal cord polyps
Authors: Jinjin Gu
Yongwang Huang
Publication date: 01-02-2017
Publisher: Springer Berlin Heidelberg
Published in: European Archives of Oto-Rhino-Laryngology / Issue 2/2017
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-016-4270-4

ACC 2024 Congress

Springer Medicine

β-Defensin-2 is overexpressed in human vocal cord polyps

Abstract

ACC 2024 Congress

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2017

MRI of diffuse large B-cell non-Hodgkin’s lymphoma of the head and neck: comparison of Waldeyer’s ring and sinonasal lymphoma

Intensive nutritional care for patients treated with radiotherapy in head and neck cancer: a randomized study and meta-analysis

Effect of hearing aid release time and presentation level on speech perception in noise in elderly individuals with hearing loss

Epithetic nasal reconstruction for nasal carcinoma: retrospective analysis on 22 patients

Comparing two lower-dose cisplatin programs for radio-chemotherapy of locally advanced head-and-neck cancers

Computed tomography findings of the bony portion of the Eustachian tube with or without patulous Eustachian tube patients