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European Archives of Oto-Rhino-Laryngology

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01-04-2015 | Rhinology

Effect of nasal sprays on an in vitro survival and morphology of nasoseptal cartilage

Authors: Katharina Stoelzel, Benjamin Kohl, Mariann Hoyer, Carola Meier, Agnieszka J. Szczepek, Heidi Olze, Gundula Schulze-Tanzil

Published in: European Archives of Oto-Rhino-Laryngology | Issue 4/2015

Abstract

Nasal sprays were introduced several years ago to support the treatment of allergic rhinitis. These sprays may come in direct contact with directly exposed nasoseptal cartilage (e.g. is case of nasoseptal perforation). To date, no studies investigated the effects of nasal sprays on cartilage tissues and cells. Therefore, our aim was to analyze the influence of two different nasal spray types (thixotropic and liposomal) on the vitality of nasoseptal chondrocytes. Human chondrocytes were isolated from surgically dissected tissues. Alternatively, nasal septa (porcine and human) tissue explants were used. The cell or explant cultures were treated with nasal sprays for 4–24 h. As a read-out, cell vitality and gene and protein expression profiles of type I and II collagen, SOX 9 and matrix metalloproteinase MMP-1 were compared to the untreated controls by means of real-time RT-PCR and immunostaining. Using the liposomal, but not thixotropic nasal spray in an explant or chondrocyte in vitro culture led to increased cell death, as compared to the untreated controls. A trend towards suppression of type II collagen and SOX 9 on protein level was found in cultures exposed to liposomal nasal spray, as compared to the controls. The thixotropic nasal spray has not affected the nasoseptal chondrocytes. Further studies with the use of viable nasoseptal cartilage explants and particularly using an in vivo animal model of exposed nasoseptal cartilage are necessary to clear the effect of liposomal spray on chondrocytes.

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Title: Effect of nasal sprays on an in vitro survival and morphology of nasoseptal cartilage
Authors: Katharina Stoelzel
Benjamin Kohl
Mariann Hoyer
Carola Meier
Agnieszka J. Szczepek
Heidi Olze
Gundula Schulze-Tanzil
Publication date: 01-04-2015
Publisher: Springer Berlin Heidelberg
Published in: European Archives of Oto-Rhino-Laryngology / Issue 4/2015
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-014-3165-5

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Springer Medicine

Effect of nasal sprays on an in vitro survival and morphology of nasoseptal cartilage

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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