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European Archives of Oto-Rhino-Laryngology

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01-12-2014 | Head and Neck

Brush biopsy with DNA-image cytometry: a useful and noninvasive method for monitoring malignant transformation of potentially malignant oral disorders

Authors: Jiang-Min Ma, Ting-Jun Zhou, Ran Wang, Jing Shan, Yu-Nong Wu, Xiao-Ling Song, Ning Gu, Yuan Fan

Published in: European Archives of Oto-Rhino-Laryngology | Issue 12/2014

Abstract

Oral and pharyngeal cancer is the sixth most common cancer worldwide, the 5-year survival rate has not yet increased. A key factor in rates not having improved is the lack of early detection. This study was undertaken to estimate the diagnostic accuracy of brush biopsy with DNA-image cytometry (a noninvasive method) for potentially malignant oral disorders compared with tissue biopsy pathology in China. Exfoliative cells were obtained using a cytobrush cell collector from oral mucosa of 52 subjects, followed by scalpel biopsy from the same region. Nuclear DNA contents (ploidy) were measured after Feulgen restaining, using an automated DNA image cytometer. Exfoliative cytology with DNA-image cytometry and histopathological diagnosis were performed separately at different institutions. Histological investigation was considered the gold standard. We reported that the sensitivity of DNA aneuploidy for the detection of cancer cells in potentially malignant oral disorders was 86.36 %, its specificity was 90.00 %, its positive predictive value was 86.36 %, and its negative predictive value was 90.00 %. Brush biopsy with DNA-image cytometry is a useful method for monitoring potentially malignant oral disorders.

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Title: Brush biopsy with DNA-image cytometry: a useful and noninvasive method for monitoring malignant transformation of potentially malignant oral disorders
Authors: Jiang-Min Ma
Ting-Jun Zhou
Ran Wang
Jing Shan
Yu-Nong Wu
Xiao-Ling Song
Ning Gu
Yuan Fan
Publication date: 01-12-2014
Publisher: Springer Berlin Heidelberg
Published in: European Archives of Oto-Rhino-Laryngology / Issue 12/2014
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-014-2935-4

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Springer Medicine

Brush biopsy with DNA-image cytometry: a useful and noninvasive method for monitoring malignant transformation of potentially malignant oral disorders

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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