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Archives of Gynecology and Obstetrics

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Open Access 01-09-2018 | Maternal-Fetal Medicine

Early missed abortion is associated with villous angiogenesis via the HIF-1α/VEGF signaling pathway

Authors: Zhifu Zhi, Wenmei Yang, Liling Liu, XiaoLi Jiang, Lihong Pang

Published in: Archives of Gynecology and Obstetrics | Issue 3/2018

Abstract

Purpose

To analyze the effects of the hypoxia-inducible factor 1-alpha (HIF-1α)/vascular endothelial growth factor (VEGF) signaling pathway on villous angiogenesis in early missed abortion.

Methods

Immunohistochemical assays were performed to detect the expression of micro-vessel density (MVD), HIF-1α, and VEGF in villous tissue samples from 30 missed abortions and 30 elective abortions in early pregnancy. We further analyzed the correlation between HIF-1α/VEGF and MVD. HTR8/SVneo cells were cultured under hypoxic (1%) or normoxic (20%) conditions, tube formation was investigated, and protein and mRNA level of HIF-1α/VEGF were determined using western blot and qRT-PCR. Finally, HIF-1α was knocked down with siRNA introduced into HTR8/SVneo cell line under hypoxia, and HIF-1α/VEGF expression and HTR8/SVneo tube formation were investigated.

Results

The expression of HIF-1α, VEGF, and MVD was lower in the missed abortion than in the elective abortion group. Correlational analysis showed that the expression of HIF-1α and VEGF was positively correlated with MVD in both groups. The levels of HIF-1α/VEGF mRNA and protein in HTR8/SVneo cells were significantly enhanced under hypoxia. HIF-1α knockdown with siRNA inhibited HIF-1α/VEGF mRNA and protein levels of HTR8/SVneo cells induced by hypoxia. Tube formation of HTR8/SVneo cells was significantly enhanced in hypoxic culture and was inhibited by HIF-1α knockdown with siRNA.

Conclusions

Our results reveal a novel role for HIF-1α/VEGF in regulating villous angiogenesis in early pregnancy and suggest that it may be a novel biomarker for missed abortion.

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Title: Early missed abortion is associated with villous angiogenesis via the HIF-1α/VEGF signaling pathway
Authors: Zhifu Zhi
Wenmei Yang
Liling Liu
XiaoLi Jiang
Lihong Pang
Publication date: 01-09-2018
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 3/2018
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-018-4802-9

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Early missed abortion is associated with villous angiogenesis via the HIF-1α/VEGF signaling pathway

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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