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Archives of Gynecology and Obstetrics
Published in: Archives of Gynecology and Obstetrics 2/2016

01-02-2016 | Maternal-Fetal Medicine

Assessment of angiogenesis modulators in pregnant women with pre-eclampsia: a case–control study

Authors: Guilhermo Justino Mundim, Marina Carvalho Paschoini, Edward Araujo Júnior, Fabricio Da Silva Costa, Virmondes Rodrigues Júnior

Published in: Archives of Gynecology and Obstetrics | Issue 2/2016

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Abstract

Purpose

This study aimed to evaluate the serum concentration of factors associated with placental angiogenesis in pre-eclamptic and normotensive pregnant women.

Methods

This was a prospective, cross-sectional, case–control study in which the pro-angiogenic factors PlGF, VEGF and IL-10, and the anti-angiogenic factors IL-6, IL-17 and TNF-α of 55 pregnant women (31 with pre-eclampsia—PE and 24 normotensive), with gestational age ≥20 weeks, were measured in maternal blood through the enzyme-linked immunosorbent assay (ELISA). The Mann–Whitney and Kruskal–Wallis tests were used for comparison between groups.

Results

Serum PIGF was reduced in the group of pregnant women with PE when compared with the normotensive women (493.2 ± 55.1 pg/mL vs. 4.4 ± 26.5 pg/mL; p < 0.001). There was no significant difference in PlGF levels in the pre-eclamptic pregnant women in relation to gestational age or proteinuria levels (p > 0.05). The serum levels of VEGF, IL-17, IL-10 and TNF-α were lower in the pregnant women with PE when compared with their normotensive peers, while the IL-6 levels were higher; however, this difference was not statistically significant (p > 0.05).

Conclusion

Serum PlGF levels were reduced in the pregnant women with PE and were unrelated to disease severity. Serum levels of VEGF, IL-17, IL-10 and TNF-α were reduced in the pre-eclamptic pregnant women when compared with their normotensive peers, without statistically significant differences.
Literature
1.
Lam C, Lim KH, Karumanchi AS (2005) Circulating angiogenic factors in phatogenesis and prediction of preeclampsia. Hypertension 46:1077–1085CrossRefPubMed
2.
Skjaerven R, Wilcox AJ, Lie RT (2002) The interval between pregnancies and the risk of preeclampsia. N Engl J Med 346:33–38CrossRefPubMed
3.
Muy-Rivera M, Vadachkoria S, Woelk GB, Qiu C, Mahomed K, Willians MA (2005) Maternal plasma VEGF, sVEGF-R1 and PlGF concentrations in preeclampitic and normotensive pregnant Zimbabwean women. Physiol Res 54:611–622PubMed
4.
Hladunewich M, Karumanchi SA, Lafayette R (2007) Pathophysiology of the clinical manifestations pre-eclampsia. Clin J Am Soc Nephrol 2:543–549CrossRefPubMed
5.
6.
Romero R, Nien JK, Espinoza J, Todem D, Fu W, Chung H et al (2008) A longitudinal study of angiogenic (placental growth factor) and anti-angiogenic (soluble endoglin and soluble VEGF receptor-1) factors in normal pregnancy and patients destined to develop preeclampsia and deliver a small-gestational-age neonate. J Matern Fetal Neonatal Med 21:9–23CrossRefPubMedPubMedCentral
7.
Yong BC, Levine RJ, Karumanchi SA (2010) Pathogenesis of preeclampsia. Annu Rev Pathol 5:173–192CrossRef
8.
Qiu Y, Hoareau-Aveilla C, Oltean S, Harper SJ, Bates DO (2009) The anti-angiogenic isoforms of VEGF in health and disease. Biochem Soc Trans 37:1207–1213CrossRefPubMedPubMedCentral
9.
Sandrim VC, Palei ACT, Eleuterio N, Tanus-Santos JE, Cavalli RC (2015) Antihypertensive therapy in preeclampsia is not modulated by VEGF polymorphisms. Arch Gynecol Obstet 291:799–803CrossRefPubMed
10.
Silva VRS, Soardi FC, Tanaka SCSV, Silva-Greco RL, Paschoini MC, Balarin AS (2015) Investigation of polymorphisms in pre-eclampsia related genes VEGF and IL1A. Arch Gynecol Obstet 291:1029–1035CrossRefPubMed
11.
Ghosh SK, Raheja S, Tuli A, Raghunandan C, Agarwal S (2013) Is serum placental growth factor more effective as a biomarker in predicting early onset preeclampsia in early second trimester than in first trimester of pregnancy? Arch Gynecol Obstet 287:865–873CrossRefPubMed
12.
13.
Jovanovic M, Kovacevic T, Stefanoska I, Vicovac L (2010) The effect of IL-6 on the trophoblast cell line HTR-8/SVNEO. Arch Biol Sci 62:531–538CrossRef
14.
Artunc-Ulkumen B, Guvenc y, Goker A, Gozukara C (2015) Maternal serum S100-B, PAPP-A and IL-6 in severe preeclampsia. Arch Gynecol Obstet [ahead of print]
15.
Ferrão MH, Pereira AC, Gersgorin HC, Paula TA, Corrêa RR (2006) Castro EC [Treatment effectiveness of hypertension during pregnancy]. Rev Assoc Med Bras 52:390–396CrossRefPubMed
16.
Santner-Nanan B, Peek MJ, Khanam R, Richarts L, Zhu E, Groth BF et al (2009) Systemic increase in the ratio between Foxp3 + and IL-17-producing CD4 + T cells in healthy pregnancy but not in preeclampsia. J Immnuol 183:7023–7030CrossRef
17.
Nakashima A, Ito M, Yoneda S, Shiozaki A, Hidaka T, Saito S (2010) Circulating and decidual TH17 cell levels in healthy pregnancy. Am J Reprod Immunol 63:104–109CrossRefPubMed
18.
19.
Grill S, Rusterholz C, Zanetti-Dasllenbach R, Tercanli S, Holzgreve W, Hahn S et al (2009) Potential markers of preeclampsia–a review. Reprod Biol Endocrinol 70:1–14
20.
(2000) Report of the National High Blood Pressure Education Program Working Group on High Blood Pressure in Pregnancy. Am J Obstet Gynecol 183:S1–S22
21.
Sibai BM (1998) Prevention of preeclampsia: a big disappointment. Am J Obstet Gynecol 179:1275–1278CrossRefPubMed
22.
Lynch A, Salmon JE (2010) Dysregulated complement activation as a common pathway of injury in preeclampsia and other pregnancy complications. Placenta 31:561–567CrossRefPubMedPubMedCentral
23.
Lapaire O, Shennan A, Stepan H (2010) The preeclampsia biomakers solubles fms-like tyrosine kinase-1 and placental growth factor: current knowledge, clinical implications and future application. Eur J Obstet Gynecol Reprod Biol 151:122–129CrossRefPubMed
24.
Holston AM, Qian C, Yu KF, Epstein FH, Karumanchi AS, Levine RJ (2009) Circulating factors in gestational proteinuria without hypertension. Am J Obstet Gynecol 200:392.e1–e10CrossRef
25.
Sharkey AM, Cooper JC, Balmforth JR, McLaren J, Clarck DE, Charnok-Jones DS et al (1996) Maternal plasma levels of vascular endothelial growth factor in normotensive pregnancies and in pregnancies complicated by preeclampsia. Eur J Clin Invest 26:1182–1185CrossRefPubMed
26.
Bersinger NA, Odegard RA (2005) Serum levels of macrophage colony stimulating vascular endothelial, and placenta growth factor in relation to later clinical onset of pre-eclampsia and small-for-gestational age birth. Am J Reprod Immunol 54:77–83CrossRefPubMed
27.
Liu F, Guo J, Tian T, Wang H, Dong F, Huang H et al (2011) Plancental trophoblasts shifted Th1/Th2 balance toward Th2 and inhibited Th17 immunity at fetomaternal interface. APMIS 119:597–604CrossRefPubMed
28.
Saito S (2010) TH17 cells and regulatory T cells: new light on pathophysiology of preeclampsia. Immunol Cell Biol 88:615–617CrossRefPubMed
29.
30.
Lewis DF, Canzoneri BJ, Wang Y (2009) Maternum circulating TNF- α levels are highly correlated with IL-10 levels, but not IL-6 and IL-8 levels, in women with pre-eclampsia. Am J Reprod Immunol 62:269–274CrossRefPubMedPubMedCentral
31.
Wu MY, Chen HF, Chen SU, Chao KH, Yang YS, Ho HN (2001) Increase in the production of interleukin-10 early after implantation is related to the success of pregnancy. Am J Reprod Immunol 46:386–392CrossRefPubMed
32.
Vitoratos N, Economou E, Iavazzo C, Panoulis K, Creatsas G (2010) Maternal serum levels of TNF-alpha and IL-6 long after delivery in preeclamptic and normotensive pregnant women. Mediators Inflamm 2010:908649CrossRefPubMedPubMedCentral
33.
Amash A, Holcberg G, Sheiner E, Huleihel M (2010) Magnesium sulfate normalizes placental interleukin-6 secretion in preeclampsia. J Interferon Cytokine Res 30:683–690CrossRefPubMed
34.
Roudsari FV, Ayati S, Ayatollahi H, Esmaeily H, Hasanzadeh M, Shahabian M et al (2009) Comparison of maternal serum Tumor Necrosis Factor-alpha (TNFα) in severe and mild preeclampsia versus normal pregnancy. Iran J Reprod Med 7:153–156
35.
Lamarca BD, Ryan MJ, Granger JP (2007) Pathophysiology during preeclampsia. Curr Hypertens Rev 9:480–485CrossRef
36.
Lockwood CJ, Yen C, Basar M, Kayisli UA, Martel M, Buhimschi I et al (2008) Preeclampsia-related inflammatory cytokines regulate interleukin-6 in human decidual cells. Am J Pathol 172:1571–1579CrossRefPubMedPubMedCentral
37.
Szarka A, Rigó-Junior J, Lázar L, Bekö G, Molvarec A (2010) Circulation cytokines, chemokines and adhesion molecules in normal pregnancy and preeclampsia determined by multiplex suspension array. BMC Immunol 11:1–9CrossRef
Metadata
Title
Assessment of angiogenesis modulators in pregnant women with pre-eclampsia: a case–control study
Authors
Guilhermo Justino Mundim
Marina Carvalho Paschoini
Edward Araujo Júnior
Fabricio Da Silva Costa
Virmondes Rodrigues Júnior
Publication date
01-02-2016
Publisher
Springer Berlin Heidelberg
Published in
Archives of Gynecology and Obstetrics / Issue 2/2016
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI
https://doi.org/10.1007/s00404-015-3823-x

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