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01-10-2006 | Short Communication

Expression of DNMT-1 in patients with atopic dermatitis

Authors: Toshinobu Nakamura, Iwao Sekigawa, Hitoshi Ogasawara, Kouichi Mitsuishi, Kayako Hira, Shigaku Ikeda, Hideoki Ogawa

Published in: Archives of Dermatological Research | Issue 5/2006

Abstract

DNA methylation is known to play an important role in gene transcription and alterations of methylation that contribute to the development of certain disorders such as cancer, immunodeficiency, and autoimmune diseases. We investigated the DNA methylation profiles in patients with atopic dermatitis (AD). Messenger RNA (mRNA) levels for DNA methyltransferase-1 (DNMT-1) in peripheral blood mononuclear cells (PBMC) were examined using a real-time quantitative polymerase chain reaction method. The levels of DNMT-1 mRNA were significantly lower in PBMC from the AD patients who had higher serum IgE levels compared with normal controls. Our observations suggest that suppression of DNMT-1 might be related to the pathogenesis of AD, especially in whom serum IgE level is high. This is the first report of DNMT-1 expression in AD patients.

Bestor TH (2000) The DNA methyltransferases of mammals. Hum Mol Genet 9:2395–2404PubMedCrossRef

Gaudet F, Hodgson JG, Eden A et al (2003) Induction of tumors in mice by genomic hypomethylation. Science 18:489–492CrossRef

Hamid Q, Boguniewicz M, Leung DY (1994) Differential in situ cytokine gene expression in acute vs. chronic atopic dermatitis. J Clin Invest 94:870–876PubMedCrossRef

Hanifin JM, Rajka G (1980) Diagnostic features of atopic dermatitis. Acta Derm Venereol 92(Suppl):44–47

Kuwabara N, Kondo N, Fukutomi O et al (1995) Methylation pattern of I epsilon region in B cells stimulated with interleukin 4 and Epstein–Barr virus in patients with a high level of serum IgE. Eur J Immunogenet 22:265–275PubMedCrossRef

Leung DYM, Tharp M, Boguniewicz M (1999) Atopic dermatitis (atopic eczema). In: Freedberg IM, Eisen AZ, Wolff K, Austen KF, Goldsmith LA, Katz SI, Fitzpatrick TB (eds) Fitzpatrick’s dermatology in general medicine, 5th edn, vol. 1. McGraw-Hill, New York, pp 1464–1480

Lorenzo PR, Kuwabara N, Kondo N, Orii T (1995) IgE production by B-cells stimulated with interleukin-4 and Epstein–Barr virus in patients with elevated serum IgE levels. J Investig Allergol Clin Immunol 5:78–81PubMed

Nakao M (2001) Epigenetics: interaction of DNA methylation and chromatin. Gene 278:25–31PubMedCrossRef

Ogasawara H, Okada M, Kaneko H et al (2003) Possible role of DNA hypomethylation in the induction of SLE: relationship to the transcription of human endogenous retroviruses. Clin Exp Rheumatol 21:733–738PubMed

10.

Ogawa H, Yoshiike T (1993) A speculative view of atopic dermatitis: barrier dysfunction in pathogenesis. J Dermatol Sci 5:197–204PubMedCrossRef

11.

Portis JL (2002) Perspectives on the role of endogenous human retroviruses in autoimmune diseases. Virology 296:1–5PubMedCrossRef

12.

Sekigawa I, Okada M, Ogasawara H et al (2003) DNA methylation in systemic lupus erythematosus. Lupus 12:79–85PubMedCrossRef

13.

Sekigawa I, Yoshiike T, Iida N et al (2003) Allergic diseases in systemic lupus erythematosus: prevalence and immunological considerations. Clin Exp Rheumatol 21:117–121PubMed

14.

Van Reijsen FC, Wijburg OL, Gebhardt M et al (1994) Different growth factor requirements for human Th2 cells may reflect in vivo induced anergy. Clin Exp Immunol 98:151–157PubMedCrossRef

15.

Yung RL, Richardson BC (1994) Role of T cell DNA methylation in lupus syndrome. Lupus 3:487–491PubMedCrossRef

Title: Expression of DNMT-1 in patients with atopic dermatitis
Authors: Toshinobu Nakamura
Iwao Sekigawa
Hitoshi Ogasawara
Kouichi Mitsuishi
Kayako Hira
Shigaku Ikeda
Hideoki Ogawa
Publication date: 01-10-2006
Publisher: Springer-Verlag
Published in: Archives of Dermatological Research / Issue 5/2006
Print ISSN: 0340-3696
Electronic ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-006-0682-0

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Expression of DNMT-1 in patients with atopic dermatitis

Abstract

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Abstract

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