Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Archives of Orthopaedic and Trauma Surgery
Published in: Archives of Orthopaedic and Trauma Surgery 6/2009

01-06-2009 | Orthopaedic Surgery

Mosaicplasty associated with gene enhanced tissue engineering for the treatment of acute osteochondral defects in a goat model

Authors: Jun Sun, Xiao-Kui Hou, Xu Li, Ting-Ting Tang, Ru-Ming Zhang, Yong Kuang, Meng Shi

Published in: Archives of Orthopaedic and Trauma Surgery | Issue 6/2009

Login to get access

Abstract

Objective

To compare single mosaicplasty, mosaicplasty associated with gene enhanced tissue engineering and mosaicplasty associated with the gels of non-gene transduced bone mesenchymal stem cells (BMSCs) in alginate for the treatment of acute osteochondral defects in a goat model.

Methods

The principle and methods of tissue engineering were used. BMSCs were separated and amplified in vitro, and human transforming growth factor-β1 (hTGF-β1) gene was transduced to the cells. Then, the cells were suspended in the alginate. At the same time using mosaicplasty to repair the defects on the medial femoral condyle, the dead space between the cylindrical grafts were filled with the gels of hTGF-β1 gene transduced BMSCs in alginate. Single mosaicplasty and mosaicplasty associated with the gels of non-gene transduced BMSCs in alginate were compared by the different time observation.

Results

All of the three treatments could repair the acute osteochondral defects. Mosaicplasty associated with gene enhanced tissue engineering had a better integration than single mosaicplasty, and mosaicplasty associated with the gels of non-gene transduced BMSCs in alginate.

Conclusion

Mosaicplasty associated with tissue engineering could solve the problem of the poor concrescence of the remnant defect and the integration of single mosaicplasty.
Literature
1.
Abernethy PJ, Townsend PR, Rose RM et al (1978) Is chondromalacia patellae a separate clinical entity? J Bone Joint Surg Br 60-B(2):205–210PubMed
2.
Hungerford DS, Barry M (1979) Biomechanics of the patellofemoral joint. Clin Orthop Relat Res 144:9–15PubMed
3.
Steinwachs MR, Guggi T, Kreuz PC (2008) Marrow stimulation techniques. Injury 39(Suppl 1):S26–S31PubMedCrossRef
4.
Steadman JR, Sterett WI (1995) The surgical treatment of knee injuries in skiers. Med Sci Sports Exerc 27(3):328–333PubMed
5.
Hangody L, Fules P (2003) Autologous osteochondral mosaicplasty for the treatment of full-thickness defects of weight-bearing joints: ten years of experimental and clinical experience. J Bone Joint Surg Am 85-A(Suppl 2):25–32PubMed
6.
Horas U, Pelinkovic D, Herr G (2003) Autologous chondrocyte implantation and osteochondral cylinder transplantation in cartilage repair of the knee joint. J Bone Joint Surg Am 85(2):185–192PubMed
7.
Onstott AT, Moczo A, Harris NL (2000) Osteochondral autotransfer—newer treatment for chondral defects. AORN J 71(4):843–845 848–851PubMedCrossRef
8.
Evans NA, Jackson DW, Simon TM (2005) MRI and histologic evaluation of two cases of osteochondral autograft transplantation procedures. J Knee Surg 18:43–48PubMed
9.
Thaunat M, Couchon S, Lunn J, Charrois O, Fallet L, Beaufils P (2007) Cartilage thickness matching of selected donor and recipient sites for osteochondral autografting of the medial femoral condyle. Knee Surg Sports Traumatol Arthrosc. 15(4):381–386 Epub 2006 Nov 10PubMedCrossRef
10.
Tibesku CO, Szuwart T, Kleffner TO, Schlegel PM, Jahn UR, Van Aken H, Fuchs S (2004) Hyaline cartilage degenerates after autologous osteochondral transplantation. J Orthop Res 22(6):1210–1214PubMedCrossRef
11.
Grande DA, Breitbart AS, Mason J, Paulino C, Laser J, Schwartz RE (1999) Cartilage tissue engineering: current limitations and solutions. Clin Orthop Relat Res 367(Suppl):S176–S185PubMedCrossRef
12.
13.
Redman SN, Oldfield SF, Archer CW (2005) Current strategies for articular cartilage repair. Eur Cell Mater 9:23–32 discussion 23–32PubMed
14.
Goodrich LR, Hidaka C, Robbins PD, Evans CH, Nixon AJ (2007) Genetic modification of chondrocytes with insulin-like growth factor-1 enhances cartilage healing in an equine model. J Bone Joint Surg Br 89(5):672–685PubMedCrossRef
15.
Pagnotto MR, Wang Z, Karpie JC, Ferretti M, Xiao X, Chu CR (2007) Adeno-associated viral gene transfer of transforming growth factor-beta1 to human mesenchymal stem cells improves cartilage repair. Gene Ther 14(10):804–813 Epub 2007 Mar 8PubMedCrossRef
16.
Guo X, Zheng Q, Yang S, Shao Z, Yuan Q, Pan Z, Tang S, Liu K, Quan D (2006) Repair of full-thickness articular cartilage defects by cultured mesenchymal stem cells transfected with the transforming growth factor beta1 gene. Biomed Mater 1(4):206–215 Epub 2006 Sep 22PubMedCrossRef
17.
Kim HT, Zaffagnini S, Mizuno S, Abelow S, Safran MR (2006) A peek into the possible future of management of articular cartilage injuries: gene therapy and scaffolds for cartilage repair. J Orthop Sports Phys Ther 36(10):765–773PubMed
18.
Björnsson S (1993) Simultaneous preparation and quantitation of proteoglycans by precipitation with alcian blue. Anal Biochem 210(2):282–291PubMedCrossRef
19.
O’Driscoll SW, Keeley FW, Salter RB (1988) Durability of regenerated articular cartilage produced by free autogenous periosteal grafts in major full-thickness defects in joint surfaces under the influence of continuous passive motion. A follow-up report at one year. J Bone Joint Surg Am 70(4):595–606PubMed
20.
Feczkó P, Hangody L, Varga J, Bartha L, Diószegi Z, Bodó G, Kendik Z, Módis L (2003) Experimental results of donor site filling for autologous osteochondral mosaicplasty. Arthroscopy 19(7):755–761PubMed
21.
Bodo G, Hangody L, Szabo Z, Peham C, Schinzel M, Girtler D, Sotonyi P (2000) Arthroscopic autologous osteochondral mosaicplasty for the treatment of subchondral cystic lesion in the medial femoral condyle in a horse. Acta Vet Hung 48:343–354PubMedCrossRef
22.
Bodo G, Kaposi AD, Hangody L, Toth J, Bakos Z, Lukacs Z, Pentek G (2001) The surgical technique and the age of the horse both influence the outcome of mosaicplasty in a cadaver equine stifle model. Acta Vet Hung 49:111–116PubMedCrossRef
23.
Szerb I, Hangody L, Duska Z, Kaposi NP (2005) Mosaicplasty: long-term follow-up. Bull Hosp Jt Dis 63(1–2):54–62PubMed
24.
Hangody L, Feczkó P, Bartha L, Bodó G, Kish G (2001) Mosaicplasty for the treatment of articular defects of the knee and ankle. Clin Orthop Relat Res 391(Suppl):S328–S336PubMedCrossRef
25.
Hangody L, Vásárhelyi G, Hangody LR, Sükösd Z, Tibay G, Bartha L, Bodó G (2008) Autologous osteochondral grafting-technique and long-term results. Injury 39(1):32–39CrossRef
26.
Gudas R, Stankevicius E, Monastyreckiene E, Pranys D, Kalesinskas RJ (2006) Osteochondral autologous transplantation versus microfracture for the treatment of articular cartilage defects in the knee joint in athletes. Knee Surg Sports Traumatol Arthrosc 14(9):834–842 Epub 2006 Mar 22PubMedCrossRef
27.
Dozin B, Malpeli M, Cancedda R, Bruzzi P, Calcagno S, Molfetta L, Priano F, Kon E, Marcacci M (2005) Comparative evaluation of autologous chondrocyte implantation and mosaicplasty: a multicentered randomized clinical trial. Clin J Sport Med 15(4):220–226PubMedCrossRef
28.
Oztürk A, Ozdemir MR, Ozkan Y (2006) Osteochondral autografting (mosaicplasty) in grade IV cartilage defects in the knee joint: 2- to 7-year results. Int Orthop 30(3):200–204 Epub 2006 Mar 8PubMedCrossRef
29.
Koulalis D, Schultz W, Heyden M, König F (2004) Autologous osteochondral grafts in the treatment of cartilage defects of the knee joint. Knee Surg Sports Traumatol Arthrosc 12(4):329–334PubMedCrossRef
30.
Haasper C, Zelle BA, Knobloch K, Jagodzinski M, Citak M, Lotz J, Krettek C, Zeichen J (2008) No mid-term difference in mosaicplasty in previously treated versus previously untreated patients with osteochondral lesions of the talus. Arch Orthop Trauma Surg 128(5):499–504 Epub 2007 Nov 27PubMedCrossRef
31.
Bentley G, Biant LC, Carrington RW (2003) A prospective, randomised comparison of autologous chondrocyte implantation versus mosaicplasty for osteochondral defects in the knee. J Bone Joint Surg Br 85(2):223–230PubMedCrossRef
32.
Hurtig MB, Fretz PB, Doige CE, Schnurr DL (1988) Effects of lesion size and location on equine articular cartilage repair. Can J Vet Res 52:137–146PubMed
33.
Lane JG, Massie JB, Ball ST, Amiel ME, Chen AC, Bae WC, Sah RL, Amiel D (2004) Follow-up of osteochondral plug transfers in a goat model: a 6-month study. Am J Sports Med 32(6):1440–1450 Epub 2004 Jul 20PubMedCrossRef
34.
Fonseca F, Balacó I. (2007) Fixation with autogenous osteochondral grafts for the treatment of osteochondritis dissecans (stages III and IV). Int Orthop Nov 24 [Epub ahead of print]
35.
Siebert CH, Schneider U, Sopka S, Wahner T, Miltner O, Niedhart C (2006) Ingrowth of osteochondral grafts under the influence of growth factors: 6-month results of an animal study. Arch Orthop Trauma Surg. 126(4):247–252 Epub 2005 Dec 15PubMedCrossRef
36.
Siebert CH, Miltner O, Weber M, Sopka S, Koch S, Niedhart C (2003) Healing of osteochondral grafts in an ovine model under the influence of bFGF. Arthroscopy 19(2):182–187PubMed
37.
Rose T, Craatz S, Hepp P, Raczynski C, Weiss J, Josten C, Lill H (2005) The autologous osteochondral transplantation of the knee: clinical results, radiographic findings and histological aspects. Arch Orthop Trauma Surg 125(9):628–637PubMedCrossRef
38.
Sharma B, Williams CG, Khan M, Manson P, Elisseeff JH (2007) In vivo chondrogenesis of mesenchymal stem cells in a photopolymerized hydrogel. Plast Reconstr Surg 119(1):112–120PubMedCrossRef
39.
Jorgensen C, Gordeladze J, Noel D (2004) Tissue engineering through autologous mesenchymal stem cells. Curr Opin Biotechnol 15(5):406–410PubMedCrossRef
40.
Wagner DR, Lindsey DP, Li KW, Tummala P, Chandran SE, Smith RL, Longaker MT, Carter DR, Beaupre GS (2008) Hydrostatic pressure enhances chondrogenic differentiation of human bone marrow stromal cells in osteochondrogenic medium. Ann Biomed Eng 36(5):813–820 Epub 2008 Feb 12PubMedCrossRef
41.
Indrawattana N, Chen G, Tadokoro M, Shann LH, Ohgushi H, Tateishi T, Tanaka J, Bunyaratvej A (2004) Growth factor combination for chondrogenic induction from human mesenchymal stem cell. Biochem Biophys Res Commun 320(3):914–919PubMedCrossRef
42.
Glansbeek HL, van Beuningen HM, Vitters EL, van der Kraan PM, van den Berg WB (1998) Stimulation of articular cartilage repair in established arthritis by local administration of transforming growth factor-beta into murine knee joints. Lab Invest 78(2):133–142PubMed
43.
Evans CH, Ghivizzani SC, Lechman ER, Mi Z, Jaffurs D, Robbins PD (1999) Lessons learned from gene transfer approaches. Arthritis Res 1(1):21–24 Epub 1999 Jul 8PubMedCrossRef
44.
van Beuningen HM, Glansbeek HL, van der Kraan PM, van den Berg WB (1998) Differential effects of local application of BMP–2 or TGF-beta 1 on both articular cartilage composition and osteophyte formation. Osteoarthritis Cartilage 6(5):306–317PubMedCrossRef
45.
Gelse K, von der Mark K, Aigner T, Park J, Schneider H (2003) Articular cartilage repair by gene therapy using growth factor-producing mesenchymal cells. Arthritis Rheum 48(2):430–441PubMedCrossRef
46.
Diduch DR, Jordan LC, Mierisch CM, Balian G (2000) Marrow stromal cells embedded in alginate for repair of osteochondral defects. Arthroscopy 16(6):571–577PubMedCrossRef
47.
Uematsu K, Hattori K, Ishimoto Y, Yamauchi J, Habata T, Takakura Y, Ohgushi H, Fukuchi T, Sato M (2005) Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactic-glycolic acid (PLGA) scaffold. Biomaterials 26(20):4273–4279PubMedCrossRef
48.
Guo X, Wang C, Zhang Y, Xia R, Hu M, Duan C, Zhao Q, Dong L, Lu J, Qing Song Y (2004) Repair of large articular cartilage defects with implants of autologous mesenchymal stem cells seeded into beta-tricalcium phosphate in a sheep model. Tissue Eng 10(11–12):1818–1829PubMedCrossRef
49.
Caplan AI, Elyaderani M, Mochizuki Y, Wakitani S, Goldberg VM (1997) Principles of cartilage repair and regeneration. Clin Orthop Relat Res 342:254–269PubMedCrossRef
50.
Solchaga LA, Goldberg VM, Caplan AI (2001) Cartilage regeneration using principles of tissue engineering. Clin Orthop Relat Res 391(Suppl):S161–S170PubMedCrossRef
51.
Trippel SB, Ghivizzani SC, Nixon AJ (2004) Gene-based approaches for the repair of articular cartilage. Gene Ther 11(4):351–359PubMedCrossRef
52.
O’Driscoll SW (2001) Preclinical cartilage repair: current status and future perspectives. Clin Orthop Relat Res 391(Suppl):S397–S401PubMedCrossRef
53.
Wang H, Kandel RA (2004) Chondrocytes attach to hyaline or calcified cartilage and bone. Osteoarthritis Cartilage 12(1):56–64PubMedCrossRef
54.
Grashoff C, Aszódi A, Sakai T et al (2003) Integrin-linked kinase regulates chondrocyte shape and proliferation. EMBO Rep 4(4):432–438PubMedCrossRef
55.
Kim HK, Moran ME, Salter RB (1991) The potential for regeneration of articular cartilage in defects created by chondral shaving and subchondral abrasion. An experimental investigation in rabbits. J Bone Joint Surg Am 73(9):1301–1315PubMed
56.
Galois L, Freyria AM, Grossin L et al (2004) Cartilage repair: surgical techniques and tissue engineering using polysaccharide- and collagen-based biomaterials. Biorheology 41(3–4):433–443PubMed
Metadata
Title
Mosaicplasty associated with gene enhanced tissue engineering for the treatment of acute osteochondral defects in a goat model
Authors
Jun Sun
Xiao-Kui Hou
Xu Li
Ting-Ting Tang
Ru-Ming Zhang
Yong Kuang
Meng Shi
Publication date
01-06-2009
Publisher
Springer-Verlag
Published in
Archives of Orthopaedic and Trauma Surgery / Issue 6/2009
Print ISSN: 0936-8051
Electronic ISSN: 1434-3916
DOI
https://doi.org/10.1007/s00402-008-0761-0

Other articles of this Issue 6/2009

Archives of Orthopaedic and Trauma Surgery 6/2009 Go to the issue

Basic Science

Do short-stemmed-prostheses induce periprosthetic fractures earlier than standard hip stems? A biomechanical ex-vivo study of two different stem designs

Orthopaedic Surgery

The anterior center edge angle in Lequesne’s false profile view: interrater correlation, dependence on pelvic tilt and correlation to anterior acetabular coverage in the sagital plane. A cadaver study

Orthopaedic Outcome Assessment

Safety, efficacy and predictive factors of efficacy of a single intra-articular injection of non-animal-stabilized-hyaluronic-acid in the hip joint: results of a standardized follow-up of patients treated for hip osteoarthritis in daily practice

Orthopaedic Surgery

Remodeling potentials of biphasic calcium phosphate granules in open wedge high tibial osteotomy

Orthopaedic Surgery

Cortical desmoid and the four clinical scenarios

Orthopaedic Outcome Assessment

Functional outcomes of bilateral hip necrosis: total hip arthroplasty versus extracorporeal shockwave