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Open Access 01-09-2014 | Correspondence

Inside Alzheimer brain with CLARITY: senile plaques, neurofibrillary tangles and axons in 3-D

Authors: Kunie Ando, Quentin Laborde, Adina Lazar, David Godefroy, Ihsen Youssef, Majid Amar, Amy Pooler, Marie-Claude Potier, Benoit Delatour, Charles Duyckaerts

Published in: Acta Neuropathologica | Issue 3/2014

Excerpt

Alzheimer’s disease (AD) is characterized by extracellular deposits of amyloid β (Aβ) peptide and intracellular tau aggregates. Examination of these lesions in post-mortem brain tissue routinely provides a two-dimensional picture of the pathology with a limited depth of field. Several tissue-clearing protocols have been recently published [1, 4‐6, 8, 10, 11, 16]. We compared the quality of immunostaining on post-mortem human brain tissues using several clearing techniques including CLARITY [4, 5], Scale [8], SeeDB [9], and 3DISCO [6]. Results were best with CLARITY. In that method, acrylamide hydrogel links the protein of the tissue and maintains its structure. Removing the lipids makes the block transparent and facilitates antibody diffusion [4, 5]. We noticed that the results were improved if ScaleA2 [8] was used to mount the block after CLARITY. …

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Title: Inside Alzheimer brain with CLARITY: senile plaques, neurofibrillary tangles and axons in 3-D
Authors: Kunie Ando
Quentin Laborde
Adina Lazar
David Godefroy
Ihsen Youssef
Majid Amar
Amy Pooler
Marie-Claude Potier
Benoit Delatour
Charles Duyckaerts
Publication date: 01-09-2014
Publisher: Springer Berlin Heidelberg
Published in: Acta Neuropathologica / Issue 3/2014
Print ISSN: 0001-6322
Electronic ISSN: 1432-0533
DOI: https://doi.org/10.1007/s00401-014-1322-y

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Inside Alzheimer brain with CLARITY: senile plaques, neurofibrillary tangles and axons in 3-D

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