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01-07-2010 | Original Paper

Balloon cells in human cortical dysplasia and tuberous sclerosis: isolation of a pathological progenitor-like cell

Authors: Shireena A. Yasin, Kate Latak, Francesca Becherini, Anita Ganapathi, Khadijah Miller, Oliver Campos, Simon R. Picker, Nelly Bier, Martin Smith, Maria Thom, Glenn Anderson, J. Helen Cross, William Harkness, Brian Harding, Thomas S. Jacques

Published in: Acta Neuropathologica | Issue 1/2010

Abstract

Neural stem cells are present in the human post-natal brain and are important in the development of brain tumours. However, their contribution to non-neoplastic human disease is less clear. We have tested the hypothesis that malformations of cortical development contain abnormal (pathological) stem cells. Such malformations are a major cause of epilepsy. Two of the most common malformations [focal cortical dysplasia (FCD) and cortical tubers] are characterised by the presence of a population of abnormal cells known as balloon cells. The identity of these cells is unknown but one hypothesis is that they are an abnormal stem cell that contributes to the pathogenesis of the malformation. We have characterised in tissue, and isolated in culture, an undifferentiated population of balloon cells from surgical resections of FCD and cortical tubers. We show that β1-integrin labels a sub-population of balloon cells with a stem cell phenotype and show for the first time that these cells can be isolated in vitro. We have characterised the immunohistochemical, morphological and ultrastructural features of these cells. This is the first isolation of an abnormal cell with features of a progenitor/stem cell from a non-neoplastic disease of the brain.

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Title: Balloon cells in human cortical dysplasia and tuberous sclerosis: isolation of a pathological progenitor-like cell
Authors: Shireena A. Yasin
Kate Latak
Francesca Becherini
Anita Ganapathi
Khadijah Miller
Oliver Campos
Simon R. Picker
Nelly Bier
Martin Smith
Maria Thom
Glenn Anderson
J. Helen Cross
William Harkness
Brian Harding
Thomas S. Jacques
Publication date: 01-07-2010
Publisher: Springer-Verlag
Published in: Acta Neuropathologica / Issue 1/2010
Print ISSN: 0001-6322
Electronic ISSN: 1432-0533
DOI: https://doi.org/10.1007/s00401-010-0677-y

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Balloon cells in human cortical dysplasia and tuberous sclerosis: isolation of a pathological progenitor-like cell

Abstract

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Abstract

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