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01-07-2004 | Regular Paper

Up-regulation of macrophage migration-inhibitory factor expression after compression-induced spinal cord injury in rats

Authors: Masao Koda, Yutaka Nishio, Masayuki Hashimoto, Takahito Kamada, Shuhei Koshizuka, Katsunori Yoshinaga, Shin Onodera, Jun Nishihira, Hideshige Moriya, Masashi Yamazaki

Published in: Acta Neuropathologica | Issue 1/2004

Abstract

Macrophage migration inhibitory factor (MIF) is a multipotential protein that acts as a pro-inflammatory cytokine, pituitary hormone, immunoregulator, and mitogen. To elucidate function of MIF in spinal cord injury, we examined expression of MIF after compression-induced spinal cord injury using Northern blot analysis, in situ hybridization and immunohistochemistry. The MIF mRNA was up-regulated in injured spinal cord, peaking 3 days after injury shown by Northern blot analysis. In situ hybridization revealed up-regulation of MIF in microglia accumulating in the lesion epicenter 3 days after injury and astrocytes around the cystic cavity 1 week after injury. Double staining showed co-localization of MIF and tomato lectin in the lesioned site, indicating that microglia accumulating to the lesion epicenter express MIF. The time course of MIF expression is different from that of previous reports about cytokine expression peaking at earlier time points; thus, it is unlikely that MIF acts as a pro-inflammatory factor in the present study. The MIF may contribute to proliferation of astrocytes around the lesioned site in spinal cord injury because of its cell proliferation-promoting property.

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Title: Up-regulation of macrophage migration-inhibitory factor expression after compression-induced spinal cord injury in rats
Authors: Masao Koda
Yutaka Nishio
Masayuki Hashimoto
Takahito Kamada
Shuhei Koshizuka
Katsunori Yoshinaga
Shin Onodera
Jun Nishihira
Hideshige Moriya
Masashi Yamazaki
Publication date: 01-07-2004
Publisher: Springer-Verlag
Published in: Acta Neuropathologica / Issue 1/2004
Print ISSN: 0001-6322
Electronic ISSN: 1432-0533
DOI: https://doi.org/10.1007/s00401-004-0853-z

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Up-regulation of macrophage migration-inhibitory factor expression after compression-induced spinal cord injury in rats

Abstract

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Abstract

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