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International Journal of Colorectal Disease
Published in: International Journal of Colorectal Disease 4/2013

01-04-2013 | Original Article

Inhibitory effects of baicalin on orthotopic xenografts of colorectal cancer cells that are deficient in a mismatch repair gene in nude mice

Authors: Bo-Lin Yang, Hong-Jin Chen, Yu-Gen Chen, Yun-Fei Gu, Shu-Peng Zhang, Qiu Lin, Yu Sun

Published in: International Journal of Colorectal Disease | Issue 4/2013

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Abstract

Objective

The aim of this article is to study the inhibitory effects of baicalin on the growth and metastasis of orthotopic xenografts consisting of human HCT-116 colorectal cancer cells that are deficient in the mismatch repair gene hMLH1 in nude mice.

Methods

A fluorescent orthotopic transplantation model of HCT-116 cells was established. The treatment groups were administered baicalin 50 mg/kg (G2), 100 mg/kg (G3), and 200 mg/kg (G4), and the negative control group (G1) was administered 5 % NaHCO3. The volume and vascular density of the primary tumors, body weights, survival conditions, and death rates of the mice were analyzed.

Results

On the 14th, 21st, and 28th days, tumor volume in the treatment groups was significantly smaller than that in the control group, and the differences were statistically significant. At the end of the experiment, the survival rate of the experimental animals in the G3 was significantly higher than that in the G1 and G4 (P < 0.05). There were no significant differences in both the weights and surface vascular densities of the primary tumor and the metastatic tumor among all groups.

Conclusion

Baicalin had a significant inhibitory effect on the growth of nude mouse orthotopic xenografts consisting of human HCT-116 colorectal tumor cells that are deficient in the mismatch repair gene hMLH1.
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Metadata
Title
Inhibitory effects of baicalin on orthotopic xenografts of colorectal cancer cells that are deficient in a mismatch repair gene in nude mice
Authors
Bo-Lin Yang
Hong-Jin Chen
Yu-Gen Chen
Yun-Fei Gu
Shu-Peng Zhang
Qiu Lin
Yu Sun
Publication date
01-04-2013
Publisher
Springer-Verlag
Published in
International Journal of Colorectal Disease / Issue 4/2013
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI
https://doi.org/10.1007/s00384-012-1562-z

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