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International Journal of Colorectal Disease

Published in:

01-10-2010 | Original Article

Nuclear expression of CXCR4 is associated with advanced colorectal cancer

Authors: Shang-Chiung Wang, Jen-Kou Lin, Huann-Sheng Wang, Shung-Haur Yang, Anna Fen-Yau Li, Shih-Ching Chang

Published in: International Journal of Colorectal Disease | Issue 10/2010

Abstract

Background and objectives

CXCR4 and its ligand, SDF-1α, play an important role in the targeted metastasis of colon cancer. In this study, we analyzed an expression of CXCR4 in clinical samples and showed that SDF-1α affected the expression of CXCR4 in colon cancer cells.

Materials and methods

A total of 388 patients with colorectal cancer (CRC) who underwent surgery in Taipei Veterans General Hospital from 2000 to 2004 were included. The expression of CXCR4 in CRC was visualized by immunohistochemistry (anti-CXCR4 mAb, R&D 12G5). HCT116, SW480, and SW620 cells were treated with SDF-1α in vitro and the CXCR4 proteins located in the cytoplasmic and nuclear compartments were separated and analyzed with western blotting.

Results

The frequency of cytoplasmic and nuclear expression of CXCR4 in colorectal cancers was 35.6% and 36.9%, respectively. Nuclear but not cytoplasmic expression of CXCR4 was associated with advanced CRC (p < 0.001) and lymphovascular invasion. However, in multivariate analysis, nuclear expression of CXCR4 did not correlate with patients' outcome. In the in vitro study, SDF-1α, stimulation of three colorectal carcinoma lines enhanced the CXCR4 nuclear expression.

Conclusion

Expression of the CXCR4 plays a role in CRC progression and may be associated with SDF-1α stimulation.

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Title: Nuclear expression of CXCR4 is associated with advanced colorectal cancer
Authors: Shang-Chiung Wang
Jen-Kou Lin
Huann-Sheng Wang
Shung-Haur Yang
Anna Fen-Yau Li
Shih-Ching Chang
Publication date: 01-10-2010
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 10/2010
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-010-0999-1

At a glance: The STEP trials

Springer Medicine

Nuclear expression of CXCR4 is associated with advanced colorectal cancer

Abstract

Background and objectives

Materials and methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background and objectives

Materials and methods

Results

Conclusion

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