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International Journal of Colorectal Disease

Published in:

01-09-2010 | Original Article

The protective effect of oral colitis-derived proteins in a murine model of inflammatory bowel disease is associated with an increase in γδ T cells in large intestinal mucosa

Authors: Yuefang Ye, Xi Jin, Min Yue, Shaohua Chen, Chaohui Yu, Youming Li

Published in: International Journal of Colorectal Disease | Issue 9/2010

Abstract

Backgrounds and aims

Oral tolerance has previously been shown effective in preventing several immune-mediated disorders in animal models. The aims of this study were to investigate the effect of oral colitis-extracted proteins (CEP) on dextran sulfate sodium (DSS)-induced colitis in BALB/c mice and to explore the relative role of the intestinal mucosal γδ T cells.

Methods

The effect of five low oral doses of CEP on colitis was evaluated by clinical manifestation and histological lesions. Serum cytokines were measured by enzyme-linked immunosorbent assay. The percentages of the intestinal mucosal γδ T cells were evaluated by flow cytometry.

Results

CEP-fed colitis mice showed less severe symptoms and histological injury than bovine serum albumin (BSA)-fed control mice. Tolerized mice developed an increase in TGF-β1 and no change in IFN-γ serum levels. Increases in TCRγδ⁺ T cells and CD8α⁺TCRγδ⁺ T cells in small intestinal mucosal lymphocytes and no quantitative change in large intestinal mucosal lymphocytes were demonstrated in colitis mice compared to untreated mice. The proportions of TCRγδ⁺ T cells and CD8α⁺TCRγδ⁺ T cells in large intestinal mucosal lymphocytes from CEP-fed colitis mice were significantly higher compared to BSA-fed controls. The disease activity index negatively correlated with the percentages of large intestinal mucosal γδ T cells. Furthermore, mucosal repair in repair-period mice was also accompanied by increases in TCRγδ⁺ T cells and CD8α⁺TCRγδ⁺ T cells in large intestinal mucosal lymphocytes.

Conclusion

Improvement of DSS-induced colitis that resulted from oral administration of colitis-extracted proteins is associated with an increase in γδ T cells in large intestinal mucosa.

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Title: The protective effect of oral colitis-derived proteins in a murine model of inflammatory bowel disease is associated with an increase in γδ T cells in large intestinal mucosa
Authors: Yuefang Ye
Xi Jin
Min Yue
Shaohua Chen
Chaohui Yu
Youming Li
Publication date: 01-09-2010
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 9/2010
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-010-0975-9

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

The protective effect of oral colitis-derived proteins in a murine model of inflammatory bowel disease is associated with an increase in γδ T cells in large intestinal mucosa

Abstract

Backgrounds and aims

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Backgrounds and aims

Methods

Results

Conclusion

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