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International Journal of Colorectal Disease

Published in:

01-04-2010 | Original Article

Low expression of chemokine receptor CCR5 in human colorectal cancer correlates with lymphatic dissemination and reduced CD8⁺ T-cell infiltration

Authors: Tim Zimmermann, Markus Moehler, Ines Gockel, George G. Sgourakis, Stefan Biesterfeld, Michaela Müller, Martin R. Berger, Hauke Lang, Peter R. Galle, Carl C. Schimanski

Published in: International Journal of Colorectal Disease | Issue 4/2010

Abstract

Background

Chemokines and their receptors have been proposed to distinctly contribute to tumor growth, dissemination, and local immune escape. The aim of this study was to evaluate the relevance of the chemokine receptor CCR5 expression for the progression of human colorectal cancer.

Methods

CCR5 expression was assessed by RT-PCR analysis in 103 colorectal cancer patients. Intensity of CCR5 expression was correlated with both tumor and patient characteristics. Infiltration of tumor margins with CD8⁺ T cells in the context of CCR5 expression was analyzed by immunohistochemistry in additional 18 colorectal cancer specimens.

Results

Human colorectal cancer revealed variable intensities of CCR5 expression ranging from absent (48/103: 47%), weak (30/103: 29%), intermediate (13/103: 13%), to strong (12/103: 12%). Absent or weak CCR5 expression was significantly associated with advanced UICC stages (P = 0.02) and lymphatic metastasis (P = 0.05). In addition, CCR5 expression positively correlated with CD8⁺ T-cell infiltration in tumor margins (P = 0.001).

Conclusion

In summary, intermediate and strong CCR5 expression was significantly associated with nonmetastatic colorectal cancer and increased CD8⁺ T-cell infiltration.

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Title: Low expression of chemokine receptor CCR5 in human colorectal cancer correlates with lymphatic dissemination and reduced CD8+ T-cell infiltration
Authors: Tim Zimmermann
Markus Moehler
Ines Gockel
George G. Sgourakis
Stefan Biesterfeld
Michaela Müller
Martin R. Berger
Hauke Lang
Peter R. Galle
Carl C. Schimanski
Publication date: 01-04-2010
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 4/2010
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-009-0868-y

At a glance: The STEP trials

Springer Medicine

Low expression of chemokine receptor CCR5 in human colorectal cancer correlates with lymphatic dissemination and reduced CD8⁺ T-cell infiltration

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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