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International Journal of Colorectal Disease

Published in:

01-03-2007 | Original Article

Tempol prevents harmful effects of remote ischemia reperfusion injury on healing of experimental colonic anastomoses

Authors: Cagatay Aydin, Zafer Teke, Faruk Aytekin, Cigdem Yenisey, Burhan Kabay, Nilufer Genc Simsek, Koray Tekin

Published in: International Journal of Colorectal Disease | Issue 3/2007

Abstract

Background and aims

Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl) is a water-soluble analogue of the spin label TEMPO. As an antioxidative agent, it is a member of nitroxides, which detoxifies superoxide and possibly other toxic radicals in vivo. In this study, we aimed to investigate whether tempol prevents harmful systemic effects of superior mesenteric ischemia-reperfusion on left colonic anastomosis in rats.

Materials and methods

Anastomosis of the left colon was performed in 30 rats that were divided into three groups each having ten animals: sham-operated control (group I), 60 min of intestinal ischemia-reperfusion by superior mesenteric artery occlusion (group II), and tempol-treated group (30 mg/kg before and after the ischemia-reperfusion (group III). On postoperative day 5, all animals were killed and anastomotic bursting pressures were measured in vivo. Tissue samples were obtained for further investigation of anastomotic hydroxyproline content, perianastomotic malondialdehyde, and glutathione levels.

Results

There was a statistically significant increase in the quantity of myeloperoxidase activity and malondialdehyde levels in group II, along with a decrease in glutathione levels, anastomotic hydroxyproline content, and bursting pressure values when compared to controls. However, all of the investigated parameters were normalized in tempol-treated animals (group III).

Conclusion

We conclude that tempol significantly prevents harmful systemic effects of reperfusion injury on colonic anastomoses in a rat model of superior mesenteric artery occlusion.

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Title: Tempol prevents harmful effects of remote ischemia reperfusion injury on healing of experimental colonic anastomoses
Authors: Cagatay Aydin
Zafer Teke
Faruk Aytekin
Cigdem Yenisey
Burhan Kabay
Nilufer Genc Simsek
Koray Tekin
Publication date: 01-03-2007
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 3/2007
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-006-0149-y

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Tempol prevents harmful effects of remote ischemia reperfusion injury on healing of experimental colonic anastomoses

Abstract

Background and aims

Materials and methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background and aims

Materials and methods

Results

Conclusion

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