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Pediatric Surgery International

Published in:

01-03-2021 | Original Article

Cell death induction (extrinsic versus intrinsic apoptotic pathway) by intestinal ischemia–reperfusion injury in rats is time-depended

Authors: Yoav Ben-Shahar, Zaid Abassi, Yulia Pollak, Tal Koppelmann, Gregory Gorelik, Igor Sukhotnik

Published in: Pediatric Surgery International | Issue 3/2021

Abstract

Purpose

We investigate the mechanism of intestinal cell apoptosis and its relation to the time of reperfusion in a rat model of intestinal ischemia–reperfusion (IR).

Methods

Rats were divided into 4 groups: Sham-24 and Sham-48 rats underwent laparotomy without an intentional ischemic intervention and were sacrificed 24 or 48 h hours later; IR-24 and IR-48 rats underwent occlusion of SMA and portal vein for 20 min followed by 24 or 48 h of reperfusion, respectively. Park’s injury score, cell proliferation and apoptosis were determined at sacrifice. Proliferation and apoptosis-related gene and protein expression were determined using Real-Time PCR, Western Blot and Immunohistochemistry.

Results

IR-24 rats demonstrated a strong increase in cell apoptosis along with an elevated Bax and decreased Bcl-2 expression and a decrease in cell proliferation (vs Sham-24). IR-48 group showed an increase in cell proliferation and a decrease in cell apoptosis compared to IR-24 animals. IR-48 rats demonstrated an increase in apoptotic rate that was accompanied by greater TNF-α mRNA, Fas mRNA and FasL mRNA compared to Sham-48 animals.

Conclusion

While cell apoptosis in IR-24 rats is regulated mainly by intrinsic apoptotic pathway, 48 h followed ischemia extrinsic apoptotic pathway is responsible for pro-apoptotic effects of IR injury.

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Title: Cell death induction (extrinsic versus intrinsic apoptotic pathway) by intestinal ischemia–reperfusion injury in rats is time-depended
Authors: Yoav Ben-Shahar
Zaid Abassi
Yulia Pollak
Tal Koppelmann
Gregory Gorelik
Igor Sukhotnik
Publication date: 01-03-2021
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Surgery International / Issue 3/2021
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-020-04817-7

At a glance: The STEP trials

Springer Medicine

Cell death induction (extrinsic versus intrinsic apoptotic pathway) by intestinal ischemia–reperfusion injury in rats is time-depended

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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