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Pediatric Surgery International

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Open Access 01-01-2019 | Original Article

Isolation and characterisation of mouse intestinal mesoangioblasts

Authors: Silvia Perin, Conor J. McCann, Paolo De Coppi, Nikhil Thapar

Published in: Pediatric Surgery International | Issue 1/2019

Abstract

Aims and objectives

Children suffering from intestinal failure (IF) endure considerable morbidity and overall have poor survival rates, complicated by the shortage of organs available for transplantation. Therefore, new therapeutic approaches are pivotal if outcomes are to be improved. Over the past years, tissue engineering (TE) has emerged as a possible alternative treatment for many congenital and acquired conditions. TE aims at creating bioengineered organs by means of combining scaffolds with appropriate cell types, which in the intestine are organised within a multilayer structure. In order to generate functional intestine, this cellular diversity and organisation will need to be recreated. While the cells for the epithelial, neural and vascular compartments have been well defined, so far, less attention has been put on the muscular compartment. More recently, mesoangioblasts (MABs) have been identified as a novel source for tissue regeneration since they are able to give rise to vascular and other mesodermal derivatives. To date MABs have not been successfully isolated from intestinal tissue. Therefore, our aim was to demonstrate the possibility of isolating MABs from adult mouse small intestine.

Materials and methods

All experiments were carried out using small intestinal tissues from C57BL/6J mice. We applied an established protocol for MAB isolation from the isolated neuromuscular layer of the small intestine. Cultured cells were stained for Ki67 to assess proliferation rates as well as for a panel of pericyte markers to determine their phenotype.

Results

Cells were successfully isolated from gut biopsies. Cultured cells showed good proliferative capacity and positivity for at least three pericytes markers found in vessels of the gut neuromuscular wall: neuron-glial antigen 2, alkaline phosphatase and platelet-derived growth factor β.

Conclusion

This proof-of-principle study lays the foundation for further characterization of MABs as a possible cell source for intestinal smooth muscle regeneration and TE.

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Title: Isolation and characterisation of mouse intestinal mesoangioblasts
Authors: Silvia Perin
Conor J. McCann
Paolo De Coppi
Nikhil Thapar
Publication date: 01-01-2019
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Surgery International / Issue 1/2019
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-018-4373-7

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Isolation and characterisation of mouse intestinal mesoangioblasts

Abstract

Aims and objectives

Materials and methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Aims and objectives

Materials and methods

Results

Conclusion

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