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Pediatric Surgery International

Published in:

01-01-2019 | Original Article

Neonatal intestinal organoids as an ex vivo approach to study early intestinal epithelial disorders

Authors: Bo Li, Carol Lee, Marissa Cadete, Hiromu Miyake, Dorothy Lee, Agostino Pierro

Published in: Pediatric Surgery International | Issue 1/2019

Abstract

Background

Adult intestinal organoids have been used to study ex vivo intestinal injury in adulthood. However, the neonatal intestinal epithelium has many unique features that are different from adult mature intestine. Establishing a neonatal ex vivo organoid model is essential to study the epithelial physiology in early postnatal development and to investigate derangements associated with disease processes during the neonatal period like necrotizing enterocolitis (NEC).

Methods

Fresh and frozen terminal ileum was harvested from mice pups on postnatal day 9. Crypts were isolated and organoids were cultured. Organoids were exposed to hypoxia and lipopolysaccharide (LPS) for 48 h to induce epithelial injury. Inflammatory cytokines and tight junction proteins were evaluated.

Results

Robust intestinal organoids can be formed from both fresh and frozen intestinal tissue of neonatal mice pups. Hypoxia and LPS administration induced intestinal inflammation and disrupted tight junctions in these neonatal intestinal organoids.

Conclusions

We have established a novel method to grow organoids from neonatal intestine. We demonstrated that these organoids respond to the injury occurring during neonatal intestinal diseases such as NEC by increasing the organoid inflammation and by disrupting the organoid barrier function. Organoids provide an ex vivo platform to study intestinal physiology and pathology during the neonatal period.

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Title: Neonatal intestinal organoids as an ex vivo approach to study early intestinal epithelial disorders
Authors: Bo Li
Carol Lee
Marissa Cadete
Hiromu Miyake
Dorothy Lee
Agostino Pierro
Publication date: 01-01-2019
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Surgery International / Issue 1/2019
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-018-4369-3

At a glance: The STEP trials

Springer Medicine

Neonatal intestinal organoids as an ex vivo approach to study early intestinal epithelial disorders

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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