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Pediatric Surgery International
Published in: Pediatric Surgery International 2/2016

01-02-2016 | Original Article

Effect of taurine on intestinal recovery following intestinal ischemia-reperfusion injury in a rat

Authors: I. Sukhotnik, I. Aranovich, Y. Ben Shahar, N. Bitterman, Y. Pollak, D. Berkowitz, D. Chepurov, A. G. Coran, A. Bitterman

Published in: Pediatric Surgery International | Issue 2/2016

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Abstract

Purpose

Taurine (TAU) is a sulfur-containing amino acid that is involved in a diverse array of biological and physiological functions, including bile salt conjugation, osmoregulation, membrane stabilization, calcium modulation, anti-oxidation, and immunomodulation. Several studies have established that treatment with TAU significantly protects cerebral, cardiac and testicular injury from ischemia–reperfusion (IR). The purpose of the present study was to examine the effect of TAU on intestinal recovery and enterocyte turnover after intestinal IR injury in rats.

Methods

Male Sprague–Dawley rats were divided into four experimental groups: (1) Sham rats that underwent laparotomy, (2) Sham-TAU rats that underwent laparotomy and were treated with intraperitoneal (IP) TAU (250 mg/kg); (3) IR-rats that underwent occlusion of both superior mesenteric artery and portal vein for 30 min followed by 48 h of reperfusion, and (4) IR-TAU rats that underwent IR and were treated with IP TAU (250 mg/kg) immediately before abdominal closure. Intestinal structural changes, Park’s injury score, enterocyte proliferation and enterocyte apoptosis were determined 24 h following IR. The expression of Bax, Bcl-2, p-ERK and caspase-3 in the intestinal mucosa was determined using Western blot and immunohistochemistry.

Results

Treatment with TAU resulted in a significant decrease in Park’s injury score compared to IR animals. IR-TAU rats also demonstrated a significant increase in mucosal weight in jejunum and ileum, villus height in jejunum and ileum and crypt depth in ileum compared to IR animals. IR-TAU rats also experienced significantly lower apoptotic indices in jejunum and ileum which was accompanied by a higher Bcl-2/Bax ratio compared to IR animals.

Conclusions

Treatment with taurine prevents gut mucosal damage and inhibits intestinal epithelial cell apoptosis following intestinal IR in a rat.
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Metadata
Title
Effect of taurine on intestinal recovery following intestinal ischemia-reperfusion injury in a rat
Authors
I. Sukhotnik
I. Aranovich
Y. Ben Shahar
N. Bitterman
Y. Pollak
D. Berkowitz
D. Chepurov
A. G. Coran
A. Bitterman
Publication date
01-02-2016
Publisher
Springer Berlin Heidelberg
Published in
Pediatric Surgery International / Issue 2/2016
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI
https://doi.org/10.1007/s00383-015-3828-3

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