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Pediatric Surgery International

Published in:

01-10-2014 | Original Article

Epimorphin expression in a rat model of pulmonary hypoplasia associated with congenital diaphragmatic hernia

Authors: İlke Akpinar, Dicle Korgun, Arzu Çetin, Akin Yesilkaya, Gungor Karaguzel, Cem Boneval, Mustafa Melikoglu

Published in: Pediatric Surgery International | Issue 10/2014

Abstract

Purpose

The pathogenesis of pulmonary hypoplasia associated with congenital diaphragmatic hernia (CDH) remains unclear. Interactions between the epithelium and surrounding mesenchyme play an important role in normal lung morphogenesis. Epimorphin, a stromal protein, plays a role in epithelial morphogenesis and lung branching, both of which are involved in pulmonary hypoplasia. In this study, we aimed to examine the relationship between epimorphin and pulmonary hypoplasia associated with CDH in an animal model.

Methods

Time-pregnant rats were exposed to nitrofen or vehicle on gestational day 9 (D9). Fetuses were harvested on D16 and D20, and were divided into control, hypoplastic lungs with CDH (CDH+), and hypoplastic lungs without CDH (CDH−). Both lungs of each fetus were removed and subjected to morphometric and molecular biologic analyses. Lung-to-body weight ratios were calculated. Pulmonary RNA was extracted, and relative mRNA level of epimorphin was determined by quantitative real-time PCR (qRT-PCR). Protein expression of epimorphin was investigated by Western blotting.

Results

In groups D16 and D20, lung-to-body weight ratios in subgroups CDH+ were significantly lower than those of controls and CDH−. The relative mRNA expression levels of epimorphin were significantly increased in both lungs in subgroup CDH+ compared with controls and CDH− on D16. Pulmonary epimorphin gene expression levels were significantly decreased in CDH+ group on D20 compared to controls. Western blotting confirmed the qRT-PCR results showing decreased pulmonary epimorphin protein expression in CDH+ hypoplastic lungs compared to controls on D20.

Conclusion

Our study shows that there is an association between the epimorphin expression and pulmonary hypoplasia associated with CDH. Although the cause–effect relationship is far from being established, epimorphin-related mechanisms have a more critical role in early (D16) developmental stage.

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Title: Epimorphin expression in a rat model of pulmonary hypoplasia associated with congenital diaphragmatic hernia
Authors: İlke Akpinar
Dicle Korgun
Arzu Çetin
Akin Yesilkaya
Gungor Karaguzel
Cem Boneval
Mustafa Melikoglu
Publication date: 01-10-2014
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Surgery International / Issue 10/2014
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-014-3579-6

At a glance: The STEP trials

Springer Medicine

Epimorphin expression in a rat model of pulmonary hypoplasia associated with congenital diaphragmatic hernia

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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