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Pediatric Surgery International

Published in:

01-12-2010 | Original Article

Expression patterns of microRNAs are altered in hypoxic human neuroblastoma cells

Authors: Tetsuya Yamagata, Jyoji Yoshizawa, Shinsuke Ohashi, Katsuhiko Yanaga, Takao Ohki

Published in: Pediatric Surgery International | Issue 12/2010

Abstract

Background/Purpose

There was a report that microRNA (miRNA) controls multiple genes. In addition, there are some reports that the presence of neoplastic cells that are hypoxic because of rapid tumor development is related to prognosis. As a step toward identifying the role of miRNA in hypoxic tumor cells, the present study was designed to determine which miRNAs have increased expression and which have decreased expression in hypoxic neuroblastoma cells.

Methods

For this study, we used seven neuroblastoma cell lines. In four with MYCN was amplified; in the other three MYCN was non-amplified. Neuroblastoma cells were cultured under hypoxic conditions. The expression levels of 662 kinds of miRNA in the hypoxic cells were quantified by gene array.

Results

We found that the expression of 85 kinds of miRNA was increased. Expression of six of these mRNAs was increased in two or more cell lines. Hsa-miR-143, -145, and -210 were each expressed in four of the seven cell lines. In addition, expression of 48 kinds of miRNA was decreased. Expression of five was decreased in two cell lines. There was no relation between the expression of miRNA and the amplification of MYCN.

Conclusion

Our results thus suggest a possible causal relation between these three miRNAs and the malignancy of neuroblastoma in hypoxic conditions.

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Title: Expression patterns of microRNAs are altered in hypoxic human neuroblastoma cells
Authors: Tetsuya Yamagata
Jyoji Yoshizawa
Shinsuke Ohashi
Katsuhiko Yanaga
Takao Ohki
Publication date: 01-12-2010
Publisher: Springer-Verlag
Published in: Pediatric Surgery International / Issue 12/2010
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-010-2700-8

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Expression patterns of microRNAs are altered in hypoxic human neuroblastoma cells

Abstract

Background/Purpose

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background/Purpose

Methods

Results

Conclusion

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