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Pediatric Surgery International
Published in: Pediatric Surgery International 12/2008

01-12-2008 | Original Article

Carbon dioxide does not affect the methylation status of prognostic important oncogenes Rassf1A and DCR2 in neuroblastoma cells

Authors: Marc Reismann, Daniel Lehmann, Matthias Quandte, Benno M. Ure, Sylvia Glüer

Published in: Pediatric Surgery International | Issue 12/2008

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Abstract

Purpose

The aim of the current study was to investigate effects of CO2 atmosphere, mimicking conditions of the pneumoperitoneum during laparoscopy, on epigenetic conditions of Rassf1A and DCR2 oncogenes in neuroblastoma cells.

Methods

SH-SY5Y neuroblastoma cells were exposed to 100% CO2 for 4 h. Cells were lysed 4, 8 and 168 h after exposure. After methylation analysis of Rassf1A and DCR2 with polymerase chain reaction, results were compared to those of physiologically incubated neuroblastoma cells.

Results

No significant changes were found after exposure to carbon dioxide compared to the control. Values of methylated Rassf1A were 12.6 ± 1.1 versus 13.2 ± 1.4 ng/μl in the controls, respectively (4 h after incubation), 12.6 ± 1.2 versus 15.1 ± 0.9 ng/μl (8 h) and 14.2 ± 1.5 versus 11.7 ± 1.3 ng/μl (168 h). DCR2 showed values of 4.6 ± 0.5 versus 3.7 ± 0.5 ng/μl (4 h), 3.8 ± 0.5 versus 4.1 ± 0.4 ng/μl (8 h) and 3.6 ± 0.4 versus 3.8 ± 0.5 ng/μl (168 h).

Conclusion

Exposure of neuroblastoma cells to 100% CO2 does not alter methylation of two prognostic relevant index genes. It seems therefore unlikely that effects on methylation levels within CO2 pneumoperitoneum lead to epigenetic changes in neuroblastoma.
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Metadata
Title
Carbon dioxide does not affect the methylation status of prognostic important oncogenes Rassf1A and DCR2 in neuroblastoma cells
Authors
Marc Reismann
Daniel Lehmann
Matthias Quandte
Benno M. Ure
Sylvia Glüer
Publication date
01-12-2008
Publisher
Springer-Verlag
Published in
Pediatric Surgery International / Issue 12/2008
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI
https://doi.org/10.1007/s00383-008-2274-x

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