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01-05-2009 | Original Paper

Evaluation of radiotherapy effect in resveratrol-treated medulloblastoma cancer stem-like cells

Authors: Kai-Hsi Lu, Yi-Wei Chen, Ping-Hsing Tsai, Ming-Long Tsai, Yi-Yen Lee, Chih-Yao Chiang, Chung-Lan Kao, Shih-Hwa Chiou, Hung-Hai Ku, Chi-Hung Lin, Yann-Jang Chen

Published in: Child's Nervous System | Issue 5/2009

Abstract

Objectives

Resveratrol (RV), a natural polyphenol derived from red wine, recently showed the potential of anticancer and radiosensitizing effects. A recent study has suggested that the cancer stem cells (CSCs) may reflect the clinical refractory malignancy of brain tumors, including medulloblastoma (MB). The aim of the present study is to investigate the possible role of RV in radiosensitivity of MB cells and MB-associated CSCs.

Materials and methods

MB-associated CSCs were isolated and cultured by serum-free medium with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). The parental MB cells and MB-CSCs were treated with RV in different concentrations and assessed for cell viability. The treatment includes RV alone, radiation alone, or radiation combined with RV.

Results

MB-CSCs selected by serum-free medium with bFGF and EGF can form 3D spheroid formation and display enhanced self-renewal and highly co-expressed “stem cell” genes (Oct-4, Nanog, Nestin, and Musashi-1) as well as antiapoptotic genes (Bcl-2 and Bcl-xL). These MB-CSCs showed significant resistance to radiotherapy as compared to the parental MB cells. Importantly, 100 μM RV could effectively inhibit the proliferation of MB-CSCs and significantly enhance the radiosensitivity in RV-treated MB-CSCs.

Conclusions

Our data suggest that RV can effectively inhibit the proliferation and tumorigenicity of MB-CSCs and significantly synergistically enhance radiosensitivity in RV-treated MB-CSCs.

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Title: Evaluation of radiotherapy effect in resveratrol-treated medulloblastoma cancer stem-like cells
Authors: Kai-Hsi Lu
Yi-Wei Chen
Ping-Hsing Tsai
Ming-Long Tsai
Yi-Yen Lee
Chih-Yao Chiang
Chung-Lan Kao
Shih-Hwa Chiou
Hung-Hai Ku
Chi-Hung Lin
Yann-Jang Chen
Publication date: 01-05-2009
Publisher: Springer-Verlag
Published in: Child's Nervous System / Issue 5/2009
Print ISSN: 0256-7040
Electronic ISSN: 1433-0350
DOI: https://doi.org/10.1007/s00381-009-0826-6

At a glance: The STEP trials

Springer Medicine

Evaluation of radiotherapy effect in resveratrol-treated medulloblastoma cancer stem-like cells

Abstract

Objectives

Materials and methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

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