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Open Access 01-01-2018 | Original Article

Polymorphisms of nucleotide factor of activated T cells cytoplasmic 2 and 4 and the risk of acute rejection following kidney transplantation

Authors: Zijie Wang, Haiwei Yang, Shuhui Si, Zhijian Han, Jun Tao, Hao Chen, Yuqiu Ge, Miao Guo, Ke Wang, Ruoyun Tan, Ji-Fu Wei, Min Gu

Published in: World Journal of Urology | Issue 1/2018

Abstract

Background

Acute rejection (AR) is a common complication of kidney transplantation. Nuclear factors of activated T cells (NFATs) are transcription factors involved in the activation of T lymphocytes, but their association with AR is unclear.

Methods

This retrospective, case–control study included 200 renal transplant recipients who were divided into the AR group (n = 69) and stable group (n = 131). Their blood samples were collected, and DNA was extracted from the whole blood. High-throughput next-generation sequencing was used to identify single nucleotide polymorphisms (SNPs) of the NFATC2 and NFATC4 genes. The correlation of these SNPs with AR was determined by logistic analysis.

Results

Seventy-one SNPs of the NFATC2 and NFATC4 genes were identified by the sequencing and Hardy–Weinberg equilibrium analyses. After adjusting for age, gender and immunosuppressive protocols, 27 SNPs were correlated with AR, of which the SNP rs2426295 of the NFATC2 gene showed a significant correlation with AR in the HET model (AA vs. AC: OR = 0.43, 95% CI = 0.19–0.98, P = 0.045), but no significant NFATC4 SNPs were identified.

Conclusions

Our study shows that the rs2426295 variant of the NFATC2 gene is significantly associated with the occurrence of AR following kidney transplantation. And patients with AA genotypes in rs2426295 are inclined to suffer from AR pathogenesis.

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Title: Polymorphisms of nucleotide factor of activated T cells cytoplasmic 2 and 4 and the risk of acute rejection following kidney transplantation
Authors: Zijie Wang
Haiwei Yang
Shuhui Si
Zhijian Han
Jun Tao
Hao Chen
Yuqiu Ge
Miao Guo
Ke Wang
Ruoyun Tan
Ji-Fu Wei
Min Gu
Publication date: 01-01-2018
Publisher: Springer Berlin Heidelberg
Published in: World Journal of Urology / Issue 1/2018
Print ISSN: 0724-4983
Electronic ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-017-2117-2

At a glance: The STEP trials

Springer Medicine

Polymorphisms of nucleotide factor of activated T cells cytoplasmic 2 and 4 and the risk of acute rejection following kidney transplantation

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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