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World Journal of Urology

Published in:

01-08-2008 | Topic Paper

Does mechanical stimulation have any role in urinary bladder tissue engineering?

Authors: Walid A. Farhat, Herman Yeger

Published in: World Journal of Urology | Issue 4/2008

Abstract

Introduction

Tissue engineering of the urinary bladder currently relies on biocompatible scaffolds that deliver biological and physical functionality with negligible risks of immunogenic or tumorigenic potential. Recent research suggests that autologous cells that are propagated in culture and seeded on scaffolds prior to implantation improve clinical outcomes. For example, normal urinary bladder development in utero requires regular filling and emptying, and current research suggests that bladders constructed in vitro may also benefit from regular mechanical stimulation. Such stimulation appears to induce favorable cellular changes, proliferation, and production of structurally suitable extracellular matrix (ECM) components essential for the normal function of hollow dynamic organs.

Materials and methods

To mimic in vivo urinary bladder dynamics, tissue bioreactors that imitate the filling and emptying of a normal bladder have been devised. A “urinary bladder tissue bioreactor” that is able to recapitulate these dynamics while providing a cellular environment that facilitates cell–cell and cell–matrix interactions normally seen in-vivo may be necessary to successfully engineer bladder tissue.

Conclusions

The validation of a urinary bladder tissue bioreactor that permits careful control of physiological conditions will generate a broad interest from researchers interested in urinary bladder physiology and tissue engineering.

Nicolaides KH, Rosen D, Rabinowitz R, Campbell S (1988) Urine production and bladder function in fetuses with open spina bifida. Fetal Ther 3:135–140PubMedCrossRef

Coplen DE, Macarak EJ, Levin RM (1994) Developmental changes in normal fetal bovine whole bladder physiology. J Urol 151:1391–1395PubMed

Baskin L, Howard PS, Macarak E (1993) Effect of mechanical forces on extracellular matrix synthesis by bovine urethral fibroblasts in vitro. J Urol 150:637–641PubMed

Baskin L, Meaney D, Landsman A, Zderic SA, Macarak E (1994) Bovine bladder compliance increases with normal fetal development. J Urol 152:692–695 discussion 696–697PubMed

Lawrenson R, Wyndaele JJ, Vlachonikolis I, Farmer C, Glickman S (2001) Renal failure in patients with neurogenic lower urinary tract dysfunction. Neuroepidemiology 20:138–143PubMedCrossRef

Khoury JM, Timmons SL, Corbel L, Webster GD (1992) Complications of enterocystoplasty. Urology 40:9–14PubMedCrossRef

McDougal WS (1992) Metabolic complications of urinary intestinal diversion. J Urol 147:1199–1208PubMed

Duel BP, Gonzalez R, Barthold JS (1998) Alternative techniques for augmentation cystoplasty. J Urol 159:998–1005PubMedCrossRef

Cartwright LM, Shou Z, Yeger H, Farhat WA (2006) Porcine bladder acellular matrix porosity: impact of hyaluronic acid and lyophilization. J Biomed Mater Res A 77(1):180–184PubMed

10.

Farhat W, Chen J, Erdeljan P, Shemtov O, Courtman D, Khoury A, Yeger H (2003) Porosity of porcine bladder acellular matrix: impact of ACM thickness. J Biomed Mater Res A 67:970–974PubMedCrossRef

11.

Atala A (2000) Tissue engineering for bladder substitution. World J Urol 18:364–370PubMedCrossRef

12.

Atala A (2000) New methods of bladder augmentation. BJU Int 85(Suppl 3):24–34 discussion 36PubMed

13.

Oberpenning F, Meng J, Yoo JJ, Atala A (1999) De novo reconstruction of a functional mammalian urinary bladder by tissue engineering. Nat Biotechnol 17:149–155PubMedCrossRef

14.

Atala A, Bauer SB, Soker S, Yoo JJ, Retik AB (2006) Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet 367:1241–1246PubMedCrossRef

15.

Metwalli AR, Colvert JR 3rd, Kropp BP (2003) Tissue engineering in urology: where are we going? Curr Urol Rep 4:156–163PubMedCrossRef

16.

Cheng HL, Chen J, Babyn PS, Farhat WA (2005) Dynamic Gd-DTPA enhanced MRI as a surrogate marker of angiogenesis in tissue-engineered bladder constructs: a feasibility study in rabbits. J Magn Reson Imaging 21:415–423PubMedCrossRef

17.

Seliktar D, Black RA, Vito RP, Nerem RM (2000) Dynamic mechanical conditioning of collagen-gel blood vessel constructs induces remodeling in vitro. Ann Biomed Eng 28:351–362PubMedCrossRef

18.

Brown AL, Brook-Allred TT, Waddell JE, White J, Werkmeister JA, Ramshaw JA, Bagli DJ, Woodhouse KA (2005) Bladder acellular matrix as a substrate for studying in vitro bladder smooth muscle–urothelial cell interactions. Biomaterials 26:529–543PubMedCrossRef

19.

Southgate J, Cross W, Eardley I, Thomas DF, Trejdosiewicz LK (2003) Bladder reconstruction—from cells to materials. Proc Inst Mech Eng [H] 217:311–316

20.

Cross WR, Eardley I, Leese HJ, Southgate J (2005) A biomimetic tissue from cultured normal human urothelial cells: analysis of physiological function. Am J Physiol Renal Physiol 289:F459–F468PubMedCrossRef

21.

Kim BS, Nikolovski J, Bonadio J, Mooney DJ (1999) Cyclic mechanical strain regulates the development of engineered smooth muscle tissue. Nat Biotechnol 17:979–983PubMedCrossRef

22.

Lu SH, Sacks MS, Chung SY, Gloeckner DC, Pruchnic R, Huard J, de Groat WC, Chancellor MB (2005) Biaxial mechanical properties of muscle-derived cell seeded small intestinal submucosa for bladder wall reconstitution. Biomaterials 26:443–449PubMedCrossRef

23.

Eastwood M, McGrouther DA, Brown RA (1998) Fibroblast responses to mechanical forces. Proc Inst Mech Eng [H] 212:85–92

24.

Eastwood M, Mudera VC, McGrouther DA, Brown RA (1998) Effect of precise mechanical loading on fibroblast populated collagen lattices: morphological changes. Cell Motil Cytoskeleton 40:13–21PubMedCrossRef

25.

Brown RA, Prajapati R, McGrouther DA, Yannas IV, Eastwood M (1998) Tensional homeostasis in dermal fibroblasts: mechanical responses to mechanical loading in three-dimensional substrates. J Cell Physiol 175:323–332PubMedCrossRef

26.

Mudera VC, Pleass R, Eastwood M, Tarnuzzer R, Schultz G, Khaw P, McGrouther DA, Brown RA (2000) Molecular responses of human dermal fibroblasts to dual cues: contact guidance and mechanical load. Cell Motil Cytoskeleton 45:1–9PubMedCrossRef

27.

Altman GH, Horan RL, Martin I, Farhadi J, Stark PR, Volloch V, Richmond JC, Vunjak-Novakovic G, Kaplan DL (2002) Cell differentiation by mechanical stress. Faseb J 16:270–272PubMed

28.

Altman GH, Lu HH, Horan RL, Calabro T, Ryder D, Kaplan DL, Stark P, Martin I, Richmond JC, Vunjak-Novakovic G (2002) Advanced bioreactor with controlled application of multi-dimensional strain for tissue engineering. J Biomech Eng 124:742–749PubMedCrossRef

29.

Haberstroh KM, Kaefer M, DePaola N, Frommer SA, Bizios R (2002) A novel in-vitro system for the simultaneous exposure of bladder smooth muscle cells to mechanical strain and sustained hydrostatic pressure. J Biomech Eng 124:208–213PubMedCrossRef

30.

Adam RM, Eaton SH, Estrada C, Nimgaonkar A, Shih SC, Smith LE, Kohane IS, Bagli D, Freeman MR (2004) Mechanical stretch is a highly selective regulator of gene expression in human bladder smooth muscle cells. Physiol Genomics 20:36–44PubMedCrossRef

31.

Park JM, Borer JG, Freeman MR, Peters CA (1998) Stretch activates heparin-binding EGF-like growth factor expression in bladder smooth muscle cells. Am J Physiol 275:C1247–C1254PubMed

32.

Baskin L, Howard PS, Macarak E (1993) Effect of physical forces on bladder smooth muscle and urothelium. J Urol 150:601–607PubMed

33.

Ratcliffe A, Niklason LE (2002) Bioreactors and bioprocessing for tissue engineering. Ann N Y Acad Sci 961:210–215PubMed

34.

Godbey WT, Atala A (2002) In vitro systems for tissue engineering. Ann N Y Acad Sci 961:10–26PubMedCrossRef

35.

Wallis MC, Yeger H et al (2006) Feasibility study of a novel urinary bladder bioreactor. Tissue Eng Part A 14(3):339–348CrossRef

Title: Does mechanical stimulation have any role in urinary bladder tissue engineering?
Authors: Walid A. Farhat
Herman Yeger
Publication date: 01-08-2008
Publisher: Springer-Verlag
Published in: World Journal of Urology / Issue 4/2008
Print ISSN: 0724-4983
Electronic ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-008-0318-4

At a glance: The STEP trials

Springer Medicine

Does mechanical stimulation have any role in urinary bladder tissue engineering?

Abstract

Introduction

Materials and methods

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Introduction

Materials and methods

Conclusions

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