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01-06-2006 | Original Article

Histamine receptors in human detrusor smooth muscle cells: physiological properties and immunohistochemical representation of subtypes

Authors: Jochen Neuhaus, Annett Weimann, Jens-Uwe Stolzenburg, Waled Dawood, Thilo Schwalenberg, Wolfgang Dorschner

Published in: World Journal of Urology | Issue 2/2006

Abstract

The potent inflammatory mediator histamine is released from activated mast cells in interstitial cystitis (IC). Here, we report on the histamine receptor subtypes involved in the intracellular calcium response of cultured smooth muscle cells (cSMC). Fura-2 was used to monitor the calcium response in cSMC, cultured from human detrusor biopsies. The distribution of histamine receptor subtypes was addressed by immunocytochemistry in situ and in vitro. Histamine stimulated a maximum of 92% of the cells (n=335), being more effective than carbachol (70%, n=920). HTMT (H1R-agonist), dimaprit (H2R) and MTH (H3R) lead to significant lower numbers of reacting cells (60, 48 and 54%). Histamine receptor immunoreactivity (H1R, H2R, H3R, H4R) was found in situ and in vitro. Histamine-induced calcium increase is mediated by distinct histamine receptors. Thus, pre-therapeutic evaluation of histamine receptor expression in IC patients may help to optimize therapy by using a patient-specific cocktail of subtype-specific histamine receptor antagonists.

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Title: Histamine receptors in human detrusor smooth muscle cells: physiological properties and immunohistochemical representation of subtypes
Authors: Jochen Neuhaus
Annett Weimann
Jens-Uwe Stolzenburg
Waled Dawood
Thilo Schwalenberg
Wolfgang Dorschner
Publication date: 01-06-2006
Publisher: Springer-Verlag
Published in: World Journal of Urology / Issue 2/2006
Print ISSN: 0724-4983
Electronic ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-006-0079-x

At a glance: The STEP trials

Springer Medicine

Histamine receptors in human detrusor smooth muscle cells: physiological properties and immunohistochemical representation of subtypes

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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