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European Radiology
Published in: European Radiology 8/2012

01-08-2012 | Oncology

Assessment of the metabolic flow phenotype of primary colorectal cancer: correlations with microvessel density are influenced by the histological scoring method

Authors: Vicky Goh, Manuel Rodriguez-Justo, Alec Engledow, Manu Shastry, Raymondo Endozo, Jacqui Peck, Marie Meagher, Stuart A. Taylor, Steve Halligan, Ashley M. Groves

Published in: European Radiology | Issue 8/2012

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Abstract

Objectives

To investigate how the histological scoring of microvessel density affects correlations between integrated 18F-FDG-PET/perfusion CT parameters and CD105 microvessel density.

Methods

A total of 53 patients were enrolled from 2007 to 2010. Integrated 18F-FDG-PET/perfusion CT was successful in 45 patients, 35 of whom underwent surgery without intervening treatment. Tumour SUVmax, SUVmean and regional blood flow (BF) were derived. Immunohistochemical staining for CD105 expression and analysis were performed for two hot spots, four hot spots and the Chalkley method. Correlations between metabolic flow parameters and CD105 expression were assessed using Spearman’s rank correlation.

Results

Mean (SD) for tumour size was 38.5 (20.5) mm, for SUVmax, SUVmean and BF it was 19.1 (4.5), 11.6 (2.5) and 85.4 (40.3) mL/min/100 g tissue, and for CD105 microvessel density it was 71.4 (23.6), 66.8 (22.9) and 6.18 (2.07) for two hot spots, four hot spots and the Chalkley method, respectively. Positive correlation between BF and CD105 expression was modest but higher for Chalkley than for four hot spots analysis (r = 0.38, P = 0.03; r = 0.33, P = 0.05, respectively). There were no significant correlations between metabolic parameters (SUVmax or SUVmean) and CD105 expression (r = 0.08–0.22, P = 0.21–0.63).

Conclusions

The histological analysis method affects correlations between tumour CD105 expression and BF but not SUVmax or SUVmean.

Key Points

• FDG-PET/perfusion CT offers new surrogate biomarkers of angiogenesis.
• Microvessel density scoring influences histopathological correlations with CT blood flow.
• Highest correlations were found with the Chalkley analysis method.
• Correlations between SUV and CD105 are not affected by the scoring method.
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Metadata
Title
Assessment of the metabolic flow phenotype of primary colorectal cancer: correlations with microvessel density are influenced by the histological scoring method
Authors
Vicky Goh
Manuel Rodriguez-Justo
Alec Engledow
Manu Shastry
Raymondo Endozo
Jacqui Peck
Marie Meagher
Stuart A. Taylor
Steve Halligan
Ashley M. Groves
Publication date
01-08-2012
Publisher
Springer-Verlag
Published in
European Radiology / Issue 8/2012
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI
https://doi.org/10.1007/s00330-012-2423-4

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