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Cancer Chemotherapy and Pharmacology

Published in:

01-04-2019 | Cervical Cancer | Original Article

SPP1 inhibition improves the cisplatin chemo-sensitivity of cervical cancer cell lines

Authors: Xing Chen, Dongsheng Xiong, Liya Ye, Huichun Yang, Shuangshuang Mei, Jinhong Wu, Shanshan Chen, Ruoran Mi

Published in: Cancer Chemotherapy and Pharmacology | Issue 4/2019

Abstract

Purpose

Cisplatin (DDP)-based chemotherapy is a standard strategy for cervical cancer, while chemoresistance remains a huge challenge. In the present study, we aimed to explore the effects of SPP1 on the proliferation and apoptosis rate of the HeLa cervical cancer cell line with cisplatin (DDP) resistance.

Methods

Microarray analysis was employed to select differentially expressed genes in cervical cancer tissues and adjacent tissues. Then, we established a DDP-resistant HeLa cell line (res-HeLa). Western blotting was used to detect SPP1 expression in both tissue and cells. After the transfection with si-SPP1 and pcDNA3.1-SPP1, colony formation and MTT assays were applied to detect cell proliferation changes. Flow cytometry was employed to detect the cell apoptosis rate. Western blotting was performed to verify the activation of PI3K/Akt signal pathway proteins related to DDP resistance.

Results

SPP1 was overexpressed in cervical cancer tissues and cell lines. Compared to normal HeLa cells, expression of SPP1 was significantly enhanced in res-HeLa cells. SPP1 knockdown resulted in repressed proliferation and enhanced apoptosis of res-HeLa cells, which could be reversed by SPP1 overexpression in HeLa cells. Additionally, downregulation of SPP1 improved the DDP sensitivity of HeLa by inhibiting the PI3K/Akt signaling pathway.

Conclusion

SPP1 inhibition could suppress proliferation, induce apoptosis and increase the DDP chemo-sensitivity of HeLa cells.

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Title: SPP1 inhibition improves the cisplatin chemo-sensitivity of cervical cancer cell lines
Authors: Xing Chen
Dongsheng Xiong
Liya Ye
Huichun Yang
Shuangshuang Mei
Jinhong Wu
Shanshan Chen
Ruoran Mi
Publication date: 01-04-2019
Publisher: Springer Berlin Heidelberg
Keywords: Cervical Cancer
Cervical Cancer
Cytostatic Therapy
Published in: Cancer Chemotherapy and Pharmacology / Issue 4/2019
Print ISSN: 0344-5704
Electronic ISSN: 1432-0843
DOI: https://doi.org/10.1007/s00280-018-3759-5

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