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World Journal of Surgery

Published in:

01-05-2010

Nerve Growth Factor-β Expression Is Associated with Lymph Node Metastasis and Nerve Infiltration in Human Hilar Cholangiocarcinoma

Authors: Lei-bo Xu, Chao Liu, Guo-quan Gao, Xian-huan Yu, Rui Zhang, Jie Wang

Published in: World Journal of Surgery | Issue 5/2010

Abstract

Background

Angiogenesis and lymphangiogenesis are important processes in the progression of malignant tumors. Previous studies have shown that nerve growth factor-β (NGF-β) can promote the initiation and progression of many tumors. In addition, vascular endothelial growth factor-C (VEGF-C) has become recognized as the most important lymphangiogenic factor. In the present study, the expression of NGF-β in human hilar cholangiocarcinoma and its relationship with lymphangiogenesis, lymph node metastasis, nerve infiltration, and VEGF-C expression was investigated.

Methods

Nerve growth factor-β and VEGF-C expression were investigated by immunohistochemistry in samples from 28 cases of hilar cholangiocarcinoma. The lymphatic vessel density (LVD) in the tumor tissue that indicated lymphangiogenesis were calculated by immunostaining with the lymphendothelial-specific antibody D2-40. The relationship between NGF-β expression and VEGF-C expression, lymphangiogenesis, lymph node metastasis, and nerve infiltration was evaluated.

Results

The overexpression of NGF-β and VEGF-C occurred in 57.1% (16/28) and 46.4% (13/28) of tumor samples, respectively. Nerve growth factor-β overexpression was highly correlated with VEGF-C overexpression (P = 0.005), LVD (P < 0.001), lymph node metastasis (P = 0.021), nerve infiltration (P = 0.019), and tumor stage (P = 0.040). Furthermore, VEGF-C overexpression was highly correlated with LVD (P < 0.001) and lymph node metastasis (P < 0.001). However, there was no statistic significance in the relation between NGF-β expression and sex (P = 0.185), age (P = 0.387), maximal tumor size (P = 0.736), Bismuth classification (P = 0.627) as well as histological grade (P = 0.203).

Conclusions

Nerve growth factor-β might promote lymph node metastasis and nerve infiltration in human hilar cholangiocarcinoma.

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Title: Nerve Growth Factor-β Expression Is Associated with Lymph Node Metastasis and Nerve Infiltration in Human Hilar Cholangiocarcinoma
Authors: Lei-bo Xu
Chao Liu
Guo-quan Gao
Xian-huan Yu
Rui Zhang
Jie Wang
Publication date: 01-05-2010
Publisher: Springer-Verlag
Published in: World Journal of Surgery / Issue 5/2010
Print ISSN: 0364-2313
Electronic ISSN: 1432-2323
DOI: https://doi.org/10.1007/s00268-010-0417-4

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Nerve Growth Factor-β Expression Is Associated with Lymph Node Metastasis and Nerve Infiltration in Human Hilar Cholangiocarcinoma

Abstract

Background

Methods

Results

Conclusions

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Abstract

Background

Methods

Results

Conclusions

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