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Aesthetic Plastic Surgery

Published in:

20-01-2022 | Original Article

Sub-Flap Use of Nano-Selenium Oxide Solution Enhances Skin Flap Viability in Rats: Study the Novel Role of mTOR and p-mTOR Expression

Authors: Omid Akhiani, Nasim Zangouie, Reza Laripour, Amir Rashidian, Mohsen Ebrahimi, Zahra Hami, Mohsen Chamanara

Published in: Aesthetic Plastic Surgery | Issue 3/2022

Abstract

Background

Nano-selenium oxide (NSeO) particles are highly noticeable due to their tissue-protective and antioxidant properties. For this purpose, the effect of NSeO was evaluated on skin flap survival and flap oxidative stress markers in rats. Also, another effect of NSeO was investigated on the expression of mTOR and p-mTOR.

Materials and Methods

Fifty rats were divided into five groups of ten. Skin flap size was 3×8 cm in all groups. Groups were: (1) Sham, (2) Flap Surgery group, (3) Flap Surgery + NSeO, (4) Flap Surgery + Rapamycin (mTOR inhibitor), (5) Flap Surgery + Rapamycin + NSeO. The flap necrosis rate was computed using the paper pattern method on day seven after surgery. After day seven, flap tissues were collected for histological evaluations. Then, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were measured. Furthermore, the expression levels of mTOR and p-mTOR were measured using the Western blot method.

Results

Treatment with NSeO significantly reduced necrosis (P<0.05). It also resulted in a decrease in MDA level (P<0.05). Histologically, NSeO reduced inflammation and increased positive signs of tissue healing (epithelialization, neovascularization, fibroblast migration, and granulation tissue). NSeO increased SOD activity significantly (P<0.05), whereas, using rapamycin reversed these effects. Also, in all groups, mTOR changes were not significant. Additionally, p-mTOR expression was significantly reduced in groups that rapamycin was injected.

Conclusion

NSeO can reduce flap necrosis and enhance tissue healing in rats. So, it can potentially be used clinically to promote tissue repair significantly, and its effects are independent of the mTOR pathway.

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Ajami M, Davoodi SH, Habibey R, Namazi N, Soleimani M, Pazoki-Toroudi H (2013) Effect of DHA+EPA on oxidative stress and apoptosis induced by ischemia-reperfusion in rat kidneys. Fundam Clin Pharmacol 27(6):593–602PubMedCrossRef

Ajami M, Eghtesadi S, Razaz JM, Kalantari N, Habibey R, Nilforoushzadeh MA, Zarrindast M, Pazoki-Toroudi H (2011) Expression of Bcl-2 and Bax after hippocampal ischemia in DHA + EPA treated rats. Neurol Sci 32(5):811–818PubMedCrossRef

Bagdas D, Cam Etoz B, Inan Ozturkoglu S, Cinkilic N, Ozyigit MO, Gul Z, Isbil Buyukcoskun N, Ozluk K, Gurun MS (2014) Effects of systemic chlorogenic acid on random-pattern dorsal skin flap survival in diabetic rats. Biol Pharm Bull 37(3):361–370PubMedCrossRef

İnce B, Bilgen F, Gündeşlioğlu AÖ, Dadacı M, Kozacıoğlu S (2016) Use of systemic rosmarinus officinalis to enhance the survival of random-pattern skin flaps. Balkan Med J 33(6):645–651PubMedPubMedCentralCrossRef

Hasanvand A, Abbaszadeh A, Darabi S, Nazari A, Gholami M, Kharazmkia A (2017) Evaluation of selenium on kidney function following ischemic injury in rats; protective effects and antioxidant activity. J Renal Inj Prev 6(2):93–98PubMedCrossRef

Akil M, Bicer M, Oztekin E, Baltaci AK, Mogulkoc R (2011) Selenium supplementation prevents lipid peroxidation caused by arduous exercise in rat brain tissue. Bratisl Lek Listy 112:314–317PubMed

Aryannejad A, Gandominejad A, Tabary M, Noroozi N, Abbasi A, Araghi F, Mohammad Jafari R, Dehpour AR (2021) Protective effect of modafinil on skin flap survival in the experimental random-pattern skin flap model in rats: The role of ATP-sensitive potassium channels and nitric oxide pathway. J Plast Reconstr Aesthet Surg 74(6):1346–1354PubMedCrossRef

Yeoh MS, Kim DD, Ghali GE (2013) Fluorescence angiography in the assessment of flap perfusion and vitality. Oral Maxillofac Surg Clin North Am 25(1):61-66

Zarch AV, Toroudi HP, Soleimani M, Bakhtiarian A, Katebi M, Djahanguiri B (2009) Neuroprotective effects of diazoxide and its antagonism by glibenclamide in pyramidal neurons of rat hippocampus subjected to ischemia-reperfusion-induced injury. Int J Neurosci 119(9):1346–1361PubMedCrossRef

10.

Mehrjerdi FZ, Aboutaleb N, Pazoki-Toroudi H, Soleimani M, AJAMI M, Khaksari M, Safari F, Habibey R (2015) The protective effect of remote renal preconditioning against hippocampal ischemia reperfusion injury: role of KATP channels. J Mol Neurosci 57(4):554–60.

11.

Habibey R, AJAMI M, Ebrahimi SA, Hesami A, Babakoohi S, Pazoki-Toroudi H (2010) Nitric oxide and renal protection in morphine-dependent rats. Free Radic Biol Med 49(6):1109–18

12.

Rahgozar M, Pazokitoroudi H, Bakhtiarian A, Djahanguiri B (2001) Diazoxide, a K(ATP) opener, accelerates restitution of ethanol or indomethacin-induced gastric ulceration in rats independent of polyamines. J Gastroenterol Hepatol 16(3):290–296PubMedCrossRef

13.

Pazoki-Toroudi HR, Hesami A, Vahidi S, Sahebjam F, Seifi B, Djahanguiri B (2003) The preventive effect of captopril or enalapril on reperfusion injury of the kidney of rats is independent of angiotensin II AT1 receptors. Fundam Clin Pharmacol 17(5):595–598PubMedCrossRef

14.

Davudi K, Volkova LI (2007) Structure of nutrition in Iranian population. Vopr Pitan 76(3):56–61PubMed

15.

Toroudi HP, Rahgozar M, Bakhtiarian A, Djahanguiri B (1999) Potassium channel modulators and indomethacin-induced gastric ulceration in rats. Scand J Gastroenterol 34(10):962–966PubMedCrossRef

16.

Habibey R, Pazoki-Toroudi H (2008) Morphine dependence protects rat kidney against ischaemia-reperfusion injury. Clin Exp Pharmacol Physiol 35(10):1209–1214PubMedCrossRef

17.

Kumar S, Tomar MS, Acharya A (2015) Carboxylic group-induced synthesis and characterization of selenium nanoparticles and its anti-tumor potential on Dalton’s lymphoma cells. Colloids Surf B Biointerfaces 126:546–552PubMedCrossRef

18.

Skalickova S, Milosavljevic V, Cihalova K, Horky P, Richtera L, Adam V (2017) Selenium nanoparticles as a nutritional supplement. Nutrition 33:83–90PubMedCrossRef

19.

Amani H, Habibey R, Shokri F, Hajmiresmail SJ, Akhavan O, Mashaghi A, Pazoki-Toroudi H (2019) Selenium nanoparticles for targeted stroke therapy through modulation of inflammatory and metabolic signaling. Sci Rep 9(1):6044PubMedPubMedCentralCrossRef

20.

Nasiri MA, Sangpour P, Yousefzadeh S, Bagheri M (2019) Elevated temperature annealed α-Fe2O3/reduced graphene oxide nanocomposite photoanode for photoelectrochemical water oxidation. J Environ Chem Eng 7(2):102999

21.

Hay N, Sonenberg N (2004) Upstream and downstream of mTOR. Genes Dev 18(16):1926–1945PubMedCrossRef

22.

Lipton JO, Sahin M (2014) The neurology of mTOR. Neuron 84(2):275–291PubMedPubMedCentralCrossRef

23.

Sabers CJ, Martin MM, Brunn GJ, Williams JM, Dumont FJ, Wiederrecht G, Abraham RT (1995) Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells. J Biol Chem 270(2):815–822PubMedCrossRef

24.

Zhao D, Yang J, Yang L (2017) Insights for oxidative stress and mTOR signaling in myocardial ischemia/reperfusion injury under diabetes. Oxid Med Cell Longev 2017:6437467PubMedPubMedCentral

25.

Cao G-D, Xu X-Y, Zhang J-W, Chen B, Xiong M-M (2016) Phosphorylated mammalian target of rapamycin p-mTOR is a favorable prognostic factor than mTOR in gastric cancer. PLoS One 11(12):e0168085

26.

Squarize CH, Castilho RM, Bugge TH, Gutkind JS(2010) Accelerated wound healing by mTOR activation in genetically defined mouse models. PLoS One 5(5):e10643.

27.

McFarlane RM, Deyoung G, Henry RA (1965) The design of a pedicle flap in the rat to study necrosis and its prevention. Plast Reconstr Surg 35:177–182PubMedCrossRef

28.

Tabary M, Aryannejad A, Noroozi N, Tavangar SM, Mohammad Jafari R, Araghi F, Dadkhahfar S, Dehpour AR (2021) Ivermectin increases random-pattern skin flap survival in rats: the novel role of GABAergic system. J Surg Res 259:431–441PubMedCrossRef

29.

Kane AJ, Barker JE, Mitchell GM, Theile DR, Romero R, Messina A, Wagh M, Fraulin FO, Morrison WA, Stewart AG (2001) Inducible nitric oxide synthase (iNOS) activity promotes ischaemic skin flap survival. Br J Pharmacol 132(8):1631–1638PubMedPubMedCentralCrossRef

30.

Lee SW, Chung SS (2010) A review of the effects of vitamins and other dietary supplements on seizure activity. Epilepsy Behav 18(3):139–150PubMedCrossRef

31.

Montero A, Munger KA, Khan RZ, Valdivielso JM, Morrow JD, Guasch A, Ziyadeh FN, Badr KF (2000) F(2)-isoprostanes mediate high glucose-induced TGF-beta synthesis and glomerular proteinuria in experimental type I diabetes. Kidney Int 58(5):1963–1972PubMedCrossRef

32.

Smith RJ, Birndorf M, Gluck G, Hammond D, Moore WD (1992) The effect of low-energy laser on skin-flap survival in the rat and porcine animal models. Plast Reconstr Surg 89(2):306–310PubMedCrossRef

33.

Kwan J, Kim CHJ, Dunn LL, Schiesser CH, Davies MJ (2016) A novel selenium compound enhances wound closure and improves microvascular perfusion: implications for wound healing. Free Radic Biol Med 100:S72–S73CrossRef

34.

Mirastschijski U, Martin A, Jorgensen LN, Sampson B, Ågren MS (2013) Zinc, copper, and selenium tissue levels and their relation to subcutaneous abscess, minor surgery, and wound healing in humans. Biol Trace Elem Res 153(1–3):76–83PubMedCrossRef

35.

Varoglu E, Seven B, Gumustekin K, Aktas O, Sahin A, Dane S (2010) The effects of vitamin E and selenium on blood flow to experimental skin burns in rats using the 133Xe clearance technique. Central European Journal of Medicine 5(2):219–223

36.

Abbaszadeh A, Tehmasebi-Foolad A, Rajabzadeh A, Beigi-Brojeni N, Zarei L (2019) Effects of chitosan/nano selenium biofilm on infected wound healing in rats. An Experimental Study. Bull Emerg Trauma 7(3):284–291PubMedCrossRef

37.

Zhou K-l, Zhang Y-h, Lin D-s, Tao X-y, Xu H-z (2016) Effects of calcitriol on random skin flap survival in rats. Sci Rep 6:18945PubMedPubMedCentralCrossRef

38.

Socha K, Kochanowicz J, Karpińska E, Soroczyńska J, Jakoniuk M, Mariak Z, Borawska MH (2014) Dietary habits and selenium, glutathione peroxidase and total antioxidant status in the serum of patients with relapsing-remitting multiple sclerosis. Nutr J 13:62PubMedPubMedCentralCrossRef

39.

Dehkordi AJ, Mohebbi AN, Aslani MR, Ghoreyshi SM (2017) Evaluation of nanoselenium (Nano-Se) effect on hematological and serum biochemical parameters of rat in experimentally lead poisoning. Hum Exp Toxicol 36(4):421–427PubMedCrossRef

40.

Shoorei H, Seghinsara A, Abedelahi A, Kiafar E, Tayefi Nasrabadi H (2018) Protective Effects of Vitamin E and Selenium on Liver Tissue Damages Induced by Electromagnetic Field: An Ultrastructural Study. Crescent Journal of Medical and Biological Science

41.

Pence BC, Delver E, Dunn DM (1994) Effects of dietary selenium on UVB-induced skin carcinogenesis and epidermal antioxidant status. J Invest Dermatol 102(5):759–761PubMedCrossRef

42.

Xing W, Guo W, Zou C-H, Fu T-T, Li X-Y, Zhu M, Qi J-H, Song J, Dong C-H, Li Z, Xiao Y, Yuan P-S, Huang H, Xu X (2015) Acemannan accelerates cell proliferation and skin wound healing through AKT/mTOR signaling pathway. J Dermatol Sci 79(2):101–109PubMedCrossRef

43.

Ala M, Mohammad Jafari R, Nematian H, Ganjedanesh MR, Dehpour AR (2020) Sodium valproate improves skin flap survival via gamma-aminobutyric acid and histone deacetylase inhibitory system. J Surg Res 246:519–526PubMedCrossRef

Title: Sub-Flap Use of Nano-Selenium Oxide Solution Enhances Skin Flap Viability in Rats: Study the Novel Role of mTOR and p-mTOR Expression
Authors: Omid Akhiani
Nasim Zangouie
Reza Laripour
Amir Rashidian
Mohsen Ebrahimi
Zahra Hami
Mohsen Chamanara
Publication date: 20-01-2022
Publisher: Springer US
Published in: Aesthetic Plastic Surgery / Issue 3/2022
Print ISSN: 0364-216X
Electronic ISSN: 1432-5241
DOI: https://doi.org/10.1007/s00266-021-02681-x

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Sub-Flap Use of Nano-Selenium Oxide Solution Enhances Skin Flap Viability in Rats: Study the Novel Role of mTOR and p-mTOR Expression

Abstract

Background

Materials and Methods

Results

Conclusion

No Level Assigned

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Materials and Methods

Results

Conclusion

No Level Assigned

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